Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MARPU_RS14350 Genome accession   NZ_CP007031
Coordinates   3312056..3313771 (+) Length   571 a.a.
NCBI ID   WP_005224057.1    Uniprot ID   W0E6V1
Organism   Marichromatium purpuratum 984     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3307056..3318771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARPU_RS14335 (MARPU_14610) - 3307782..3308459 (+) 678 WP_005224061.1 3'-5' exonuclease -
  MARPU_RS14340 (MARPU_14615) acs 3308710..3310656 (+) 1947 WP_005224059.1 acetate--CoA ligase -
  MARPU_RS14345 (MARPU_14620) - 3311107..3311961 (+) 855 WP_005224058.1 NYN domain-containing protein -
  MARPU_RS14350 (MARPU_14625) pilB 3312056..3313771 (+) 1716 WP_005224057.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MARPU_RS14355 (MARPU_14630) pilC 3313778..3315040 (+) 1263 WP_005224056.1 type II secretion system F family protein Machinery gene
  MARPU_RS14360 (MARPU_14635) pilD 3315098..3315967 (+) 870 WP_005224055.1 A24 family peptidase Machinery gene
  MARPU_RS14365 (MARPU_14640) coaE 3315964..3316590 (+) 627 WP_005224054.1 dephospho-CoA kinase -
  MARPU_RS14370 (MARPU_14645) - 3316666..3316920 (+) 255 WP_005224053.1 hypothetical protein -
  MARPU_RS14375 (MARPU_14650) - 3317093..3318697 (+) 1605 WP_005224052.1 phosphoenolpyruvate carboxykinase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63101.09 Da        Isoelectric Point: 4.9957

>NTDB_id=116099 MARPU_RS14350 WP_005224057.1 3312056..3313771(+) (pilB) [Marichromatium purpuratum 984]
MSLDAANVKLSGLALRLVRDELLSEQQAVDAQEEATRQRRTFVGHLVGQGLLSSRSIAVAAAHEFGVPLIDLGSVEVSEL
PTSLVDERLIRKHHALPLFRRGNRLFLAVSDPTNDQALEEIRFNTGLATDAVLVEEDKLQAAIDSAIEAQDTTMSEIMDA
DLEKLEIAQDEDEERDDGSEANIDEAPIVRYVNKILVDAITAGASDIHFEPYEKFFRVRFRQDGLLQEVANPPQSIANRL
IARLKVMSRMNIAERRIPQDGRIKLKLSRSRDIDFRVNTLPTLYGEKVVLRILDSSSAQVGIDALGFEPEQRDTFLKAIK
KPYGMILVTGPTGSGKTVSLYTALNILNEPEINISTVEDPVEIQVPGINQVNMNPKTGLTFASALRAFLRQDPDIVMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLANMGVAPFNIASSVLLIMAQRLARRLCLHCREPHELPPEALRAE
GFSDEEIEAGLTIYKPVGCERCTKGYKGRVGIFQVMPISEEISRLILEGGNSMQLADQARREGVADLRRSGLRKVKNGLT
SLEELNRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=116099 MARPU_RS14350 WP_005224057.1 3312056..3313771(+) (pilB) [Marichromatium purpuratum 984]
ATGTCGCTAGATGCCGCCAACGTCAAGCTCTCGGGACTGGCCTTGCGCTTGGTCAGGGACGAGTTGCTCAGCGAGCAGCA
GGCGGTCGATGCACAGGAAGAAGCGACCCGTCAGCGGCGGACCTTCGTCGGTCATCTGGTCGGCCAGGGGCTGCTCTCGA
GCCGCTCGATCGCGGTCGCTGCGGCGCACGAGTTCGGCGTGCCGCTGATCGACCTCGGCTCGGTCGAGGTCTCCGAGCTG
CCGACCAGCCTGGTCGACGAGCGACTGATCCGCAAACACCATGCACTGCCGCTGTTCCGACGCGGCAATCGACTGTTCCT
CGCCGTCTCCGACCCCACCAACGACCAGGCGCTCGAGGAGATCCGCTTCAACACCGGGCTGGCCACCGACGCGGTGCTGG
TCGAGGAGGACAAGCTCCAGGCGGCGATCGACAGCGCGATCGAGGCCCAGGACACGACCATGAGCGAGATCATGGACGCC
GACCTGGAGAAGCTCGAGATCGCCCAGGACGAGGACGAAGAACGCGACGACGGCAGCGAGGCCAATATCGACGAGGCGCC
GATCGTGCGCTACGTCAACAAGATCCTGGTCGACGCCATCACCGCCGGGGCCTCGGACATCCATTTCGAGCCCTACGAGA
AGTTCTTCCGTGTGCGCTTCCGCCAGGACGGCCTGCTCCAGGAAGTGGCCAACCCGCCGCAGAGCATCGCCAACCGGCTG
ATCGCCCGACTCAAGGTGATGTCGCGGATGAACATCGCCGAGCGGCGCATCCCCCAGGACGGGCGCATCAAGCTCAAGCT
CAGCCGCTCGCGCGACATCGACTTCCGCGTCAACACCCTGCCCACCCTCTACGGCGAGAAGGTGGTGCTGCGTATCCTCG
ACTCGTCCTCGGCGCAGGTCGGCATCGACGCGCTCGGTTTCGAGCCCGAGCAGCGCGACACCTTCCTCAAGGCGATCAAG
AAGCCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTCTCGCTCTACACCGCGCTCAACATCCT
CAACGAACCCGAGATCAACATCTCCACCGTCGAGGATCCGGTCGAGATCCAGGTGCCCGGCATCAACCAGGTCAACATGA
ACCCCAAGACCGGGCTGACCTTCGCCTCGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCGTCATGGTCGGCGAG
ATCCGCGACCTCGAGACCGCCGAGATCGCCGTCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAA
CGACGCCCCCCAGACCCTCACCCGTCTGGCCAACATGGGCGTGGCGCCGTTCAACATCGCCTCCTCGGTACTGCTGATCA
TGGCCCAGCGACTGGCGCGCCGGCTCTGCCTGCACTGTCGCGAACCGCACGAGCTGCCGCCCGAGGCGCTGCGCGCCGAG
GGCTTCAGCGACGAGGAGATCGAGGCCGGGCTGACCATCTACAAGCCGGTCGGCTGCGAGCGCTGCACCAAGGGCTACAA
GGGGCGCGTTGGTATCTTCCAGGTGATGCCCATCTCCGAGGAGATCAGCCGGCTGATCCTCGAGGGTGGCAACTCGATGC
AGCTCGCCGACCAGGCGCGGCGCGAGGGCGTCGCCGACCTGCGCCGGTCTGGTCTGCGCAAGGTCAAGAACGGGCTCACC
AGCCTCGAAGAACTCAACCGCGTCACCAAAGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0E6V1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.846

100

0.56
  pilB Acinetobacter baylyi ADP1

54.704

100

0.55
  pilB Acinetobacter baumannii D1279779

54.325

100

0.55
  pilF Neisseria gonorrhoeae MS11

49.381

98.949

0.489
  pilB Vibrio campbellii strain DS40M4

52.075

92.82

0.483
  pilB Vibrio cholerae strain A1552

53.516

89.667

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.82

0.48
  pilF Thermus thermophilus HB27

42.173

95.096

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.322

93.52

0.396

Multiple sequence alignment