Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MARPU_RS14360 Genome accession   NZ_CP007031
Coordinates   3315098..3315967 (+) Length   289 a.a.
NCBI ID   WP_005224055.1    Uniprot ID   W0E799
Organism   Marichromatium purpuratum 984     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3310098..3320967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARPU_RS14345 (MARPU_14620) - 3311107..3311961 (+) 855 WP_005224058.1 NYN domain-containing protein -
  MARPU_RS14350 (MARPU_14625) pilB 3312056..3313771 (+) 1716 WP_005224057.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MARPU_RS14355 (MARPU_14630) pilC 3313778..3315040 (+) 1263 WP_005224056.1 type II secretion system F family protein Machinery gene
  MARPU_RS14360 (MARPU_14635) pilD 3315098..3315967 (+) 870 WP_005224055.1 A24 family peptidase Machinery gene
  MARPU_RS14365 (MARPU_14640) coaE 3315964..3316590 (+) 627 WP_005224054.1 dephospho-CoA kinase -
  MARPU_RS14370 (MARPU_14645) - 3316666..3316920 (+) 255 WP_005224053.1 hypothetical protein -
  MARPU_RS14375 (MARPU_14650) - 3317093..3318697 (+) 1605 WP_005224052.1 phosphoenolpyruvate carboxykinase -
  MARPU_RS14380 (MARPU_14655) pyrC 3318703..3319743 (+) 1041 WP_005224051.1 dihydroorotase -
  MARPU_RS14385 (MARPU_14660) - 3319801..3320559 (-) 759 WP_005224050.1 VacJ family lipoprotein -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31189.16 Da        Isoelectric Point: 8.0555

>NTDB_id=116101 MARPU_RS14360 WP_005224055.1 3315098..3315967(+) (pilD) [Marichromatium purpuratum 984]
MPWLEPFQQSPMLLYVTVTLLGLILGSFLNVVILRLPRMLEHEWRAQCAEFAGESADATPPPGLARPPSTCPHCGHRIRA
HENIPLLSFLLLRGRCSACKTPIGWRYPAVELLTALLGLVAALHFGASVQLLAALVLTWGLVALAVIDLDTQLLPDRITL
PLLWLGLILSLFGVFTDAQAAIIGAAAGYLLLWSLYHAFRLLTGKEGMGYGDFKLLALFGAWLGWQALPLVILLSAVSGA
LIGGVLIALRRQGRDTAMPFGPFLAVAGWVALLWGEQITGAYLRLSGLG

Nucleotide


Download         Length: 870 bp        

>NTDB_id=116101 MARPU_RS14360 WP_005224055.1 3315098..3315967(+) (pilD) [Marichromatium purpuratum 984]
ATGCCCTGGCTAGAGCCATTCCAGCAGTCCCCCATGCTGCTCTATGTCACCGTCACGCTGCTCGGACTGATCCTCGGCAG
CTTCCTCAACGTCGTCATCCTGCGTCTGCCGCGGATGCTCGAGCACGAGTGGCGCGCGCAGTGCGCCGAGTTCGCCGGCG
AGTCCGCCGACGCGACGCCGCCCCCAGGGCTGGCGCGCCCGCCCTCGACCTGCCCGCACTGCGGTCACCGCATCCGCGCC
CACGAGAACATCCCGCTGCTGAGCTTCCTGCTGCTGCGCGGACGCTGCAGCGCCTGCAAGACGCCGATCGGTTGGCGCTA
TCCGGCCGTCGAGCTGCTCACCGCGTTGCTCGGTCTCGTCGCGGCGCTGCACTTCGGCGCCAGTGTGCAGCTACTCGCCG
CGCTGGTGCTGACCTGGGGCCTGGTCGCACTGGCGGTGATCGACCTCGACACCCAGCTGCTGCCCGACCGCATCACCCTG
CCGCTGCTGTGGCTCGGGCTGATCCTCAGCCTGTTCGGGGTGTTCACCGACGCCCAGGCGGCGATCATCGGCGCGGCTGC
CGGCTATCTGTTGCTGTGGAGCCTCTATCACGCCTTTCGCCTGCTCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCA
AGCTGCTGGCCCTGTTCGGGGCCTGGCTCGGCTGGCAGGCGCTGCCCCTGGTGATACTGCTCTCGGCGGTCTCCGGGGCG
CTGATTGGTGGCGTGCTGATCGCACTGCGACGCCAGGGGCGCGACACCGCCATGCCCTTCGGTCCCTTCCTCGCCGTCGC
CGGCTGGGTCGCACTGCTGTGGGGCGAGCAGATCACCGGCGCCTATCTGCGCCTCAGCGGACTGGGATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-243)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0E799

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.986

98.27

0.55
  pilD Vibrio campbellii strain DS40M4

54.064

97.924

0.529
  pilD Acinetobacter nosocomialis M2

51.254

96.54

0.495
  pilD Acinetobacter baumannii D1279779

50.538

96.54

0.488
  pilD Neisseria gonorrhoeae MS11

47.126

90.311

0.426

Multiple sequence alignment