Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG37_RS02580 Genome accession   NZ_CP010143
Coordinates   489927..491312 (-) Length   461 a.a.
NCBI ID   WP_073503172.1    Uniprot ID   -
Organism   Escherichia coli strain D4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484927..496312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG37_RS02550 (RG37_02400) mutT 485257..485646 (+) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -
  RG37_RS02555 (RG37_02410) yacG 485862..486059 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RG37_RS02560 (RG37_02415) zapD 486069..486812 (-) 744 WP_001194734.1 cell division protein ZapD -
  RG37_RS02565 (RG37_02420) coaE 486812..487432 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  RG37_RS27120 - 487457..487501 (+) 45 WP_120795372.1 protein YacM -
  RG37_RS02570 (RG37_02425) guaC 487657..488700 (+) 1044 WP_001217338.1 GMP reductase -
  RG37_RS02575 (RG37_02430) hofC 488735..489937 (-) 1203 WP_000157266.1 protein transport protein HofC -
  RG37_RS02580 (RG37_02435) pilB 489927..491312 (-) 1386 WP_073503172.1 type II secretion system protein GspE Machinery gene
  RG37_RS02585 (RG37_02440) pilA 491322..491762 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RG37_RS02595 (RG37_02450) nadC 491965..492858 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG37_RS02600 (RG37_02455) ampD 492946..493497 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG37_RS02605 (RG37_02460) ampE 493494..494348 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RG37_RS02610 (RG37_02465) aroP 494391..495764 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50629.36 Da        Isoelectric Point: 6.6612

>NTDB_id=116063 RG37_RS02580 WP_073503172.1 489927..491312(-) (pilB) [Escherichia coli strain D4]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDINTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=116063 RG37_RS02580 WP_073503172.1 489927..491312(-) (pilB) [Escherichia coli strain D4]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATATCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.65

100

0.447
  pilB Legionella pneumophila strain ERS1305867

50.26

83.297

0.419
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.923

84.599

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Thermus thermophilus HB27

40.587

88.72

0.36

Multiple sequence alignment