Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QOT06_RS02155 Genome accession   NZ_OX461117
Coordinates   512611..514323 (-) Length   570 a.a.
NCBI ID   WP_283627632.1    Uniprot ID   -
Organism   Shewanella baltica strain SF1039 isolate SF1039     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507611..519323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOT06_RS02125 mutT 508114..508506 (-) 393 WP_259561568.1 8-oxo-dGTP diphosphatase MutT -
  QOT06_RS02130 yacG 508583..508792 (-) 210 WP_006083355.1 DNA gyrase inhibitor YacG -
  QOT06_RS02135 zapD 508866..509600 (-) 735 WP_283627626.1 cell division protein ZapD -
  QOT06_RS02140 coaE 509593..510210 (-) 618 WP_283627627.1 dephospho-CoA kinase -
  QOT06_RS02145 pilD 510250..511164 (-) 915 WP_283627628.1 A24 family peptidase Machinery gene
  QOT06_RS02150 pilC 511206..512477 (-) 1272 WP_283627630.1 type II secretion system F family protein Machinery gene
  QOT06_RS02155 pilB 512611..514323 (-) 1713 WP_283627632.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QOT06_RS02160 - 514770..516131 (+) 1362 WP_283627634.1 O-antigen ligase family protein -
  QOT06_RS02165 - 516393..516806 (-) 414 Protein_420 IS4 family transposase -
  QOT06_RS02170 - 516903..518027 (+) 1125 WP_283627348.1 ISAs1 family transposase -
  QOT06_RS02175 - 518052..518877 (-) 826 Protein_422 IS4 family transposase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62785.25 Da        Isoelectric Point: 6.2567

>NTDB_id=1159814 QOT06_RS02155 WP_283627632.1 512611..514323(-) (pilB) [Shewanella baltica strain SF1039 isolate SF1039]
MPSTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVTTLVQSKLVSARAIAELCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDRRNEESAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQAQITQGFTTFKPVGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVIHGVTSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1159814 QOT06_RS02155 WP_283627632.1 512611..514323(-) (pilB) [Shewanella baltica strain SF1039 isolate SF1039]
ATGCCGTCTACAGGCCTACATTTGGGTTTATCGACCCTATTTATCCGTAAAGGTCTGCTAAATGAAGAGCAGATGGCAAA
TGCCATCACAAAATCCCGTCAAAATAAACACGCCCTTGTTACTACTTTAGTACAAAGTAAACTGGTTTCTGCTCGCGCTA
TTGCCGAACTTTGCTACGAAGAATACGGTACACCACTACTCGATCTTAATGAGTTTGATCTTGCTTCCATACCAGAGGAT
TTTCTCAATAAAAAACTCATTGAAAAACATCGCTGCTTACCCTTGTTTAAACGCGGTAATCGCCTCTATATCGGCACATC
CGACCCAACCAATATTGCCGCCCTCGAAGATTTCCAATTTAGTGCAGGTCTGCACGCCGAAGCGATTCTAGTTGAGGAAG
ATAAACTCGCTAAAGCACTAGAGAAAATCCTCGAAGAAGATATCAGCGGTCTCGATTTAGGCGGCATGGATGAAGCAGCG
CTGGCAGCGATAGAAATTACTGATACCGACAGACGTAATGAGGAGTCCGCAGGCGAAGCTAGCGACGATGCGCCTATTGT
TATCTATATCAATAAAATATTGACAGATGCCATCCGTAAGGGCGCGTCGGATTTACACTTTGAACCCTATGAGAAACGCT
ACCGTATTCGTTTCCGTATAGATGGTATTCTGCACGAAGTCTCTGAACCGCCGATTAGCCTCGCAGGTCGTTTATCGGCG
CGACTAAAGGTAATGTCGAAACTCGATATAGCCGAACGGCGGGTACCTCAGGATGGTCGAATTAAGATGAAGCTTTCCCG
TACTAAGTCCATCGACTTTCGGGTAAGTACCCTACCGACACTGTGGGGGGAAAAAATTGTAATGCGGATTTTGGACTCAT
CCTCCGCACAATTAGGTATTGAAAAGCTTGGCTACGAGCCCGACCAAGAAAAACTCTACCTTGAGATGCTGGCAAAACCA
CAAGGGATGATTCTAGTGACGGGGCCAACGGGTTCAGGTAAAACAGTTTCCCTCTATACAGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATTTCTACCGCAGAAGATCCGGTTGAGATTAACCTTGAAGGCGTGAACCAAGTTCACATTAATTTAA
AGGCAGGGTTAACCTTCGCATCCGCTTTACGTTCATTTCTACGCCAAGACCCAGATGTGGTGATGGTGGGTGAAATTCGC
GATCTTGAAACCGCCGAAATTGCCATTAAAGCAGCACAAACGGGTCACTTAGTCTTATCGACCTTGCACACAAACTCGGC
TGCCGAAACCTTAACCCGTTTAATCAACATGGGTGTACCTGGTTACAACATAGCCAGCTCAGTGAACCTGATTATTGCCC
AGCGCTTAGCGCGTCGCCTATGCCCTGAATGCAAACAAGCGGAAGTAATACCAGAACATGAGCTACTGCGCCTAGGTTTT
GCCCAAGCGCAAATTACTCAGGGCTTTACCACCTTTAAACCCGTTGGCTGTGAACATTGCTCTGCGGGCTACAAGGGACG
AGTTGGCATTTATGAGGTCATGAAGATGTCTGACGAAATAGCCCGTACTATCATGGAAGGCGGCAACTCTTTGCAAATCG
CCTCCCAAGCTAAAGCGCAAGGAATGCGAGATTTGCGTCAATCAGGTTTATTGAAAGTGATCCACGGTGTCACTAGCATT
GCAGAAGTCAATCGTGTAACCAGTTTTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.421

0.584
  pilB Acinetobacter baumannii D1279779

59.083

95.614

0.565
  pilB Legionella pneumophila strain ERS1305867

56.05

98.596

0.553
  pilB Vibrio cholerae strain A1552

53.153

97.368

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.585

99.649

0.514
  pilB Vibrio campbellii strain DS40M4

51.327

99.123

0.509
  pilF Neisseria gonorrhoeae MS11

47.88

99.298

0.475
  pilF Thermus thermophilus HB27

39.046

99.298

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.53

97.895

0.377

Multiple sequence alignment