Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N018_RS01915 Genome accession   NZ_CP007014
Coordinates   409757..411814 (+) Length   685 a.a.
NCBI ID   WP_024646244.1    Uniprot ID   -
Organism   Pseudomonas syringae CC1557     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 404757..416814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N018_RS01895 (N018_01910) pilM 406926..407990 (+) 1065 WP_024646248.1 pilus assembly protein PilM Machinery gene
  N018_RS01900 (N018_01915) - 407990..408565 (+) 576 WP_024646247.1 PilN domain-containing protein -
  N018_RS01905 (N018_01920) pilO 408562..409185 (+) 624 WP_024646246.1 type 4a pilus biogenesis protein PilO -
  N018_RS01910 (N018_01925) pilP 409182..409709 (+) 528 WP_024646245.1 type 4a pilus biogenesis lipoprotein PilP -
  N018_RS01915 (N018_01930) pilQ 409757..411814 (+) 2058 WP_024646244.1 type IV pilus secretin PilQ Machinery gene
  N018_RS01920 (N018_01935) aroK 411819..412337 (+) 519 WP_007248056.1 shikimate kinase AroK -
  N018_RS01925 (N018_01940) aroB 412454..413557 (+) 1104 WP_025388710.1 3-dehydroquinate synthase -
  N018_RS01930 (N018_01945) - 413568..415192 (+) 1625 Protein_389 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 685 a.a.        Molecular weight: 73858.13 Da        Isoelectric Point: 5.1783

>NTDB_id=115712 N018_RS01915 WP_024646244.1 409757..411814(+) (pilQ) [Pseudomonas syringae CC1557]
MTRILSIIGASLCIAMLSPVVQAATLKTLDVAALPGDRIELKLSFDAPVPVPRGYTTAQPARIALDLPGVTSQLATKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGATAAQASQPSTALGASRVAVPPPTPASARPFVPA
GAKAIRNIDFQRGELGEGNVVIDLSNANIAPDIQEQGGKIRVDFAKTMLPEPLRVRLDVKDFATPVQFVSASAAGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTHEDMDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKIGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKELGVRWGGASNTSGDS
NWSTGRNNSTFVDMGAANATSGIGLGFLTNNTMLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEVPYQESSSSG
ATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNAVLGVPPIKKNEVNAKVLISDGETIVIGGVFSNTQSKSVD
KVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2058 bp        

>NTDB_id=115712 N018_RS01915 WP_024646244.1 409757..411814(+) (pilQ) [Pseudomonas syringae CC1557]
ATGACCCGGATTCTCTCGATTATCGGCGCGTCGTTATGCATCGCGATGCTGTCTCCGGTTGTCCAGGCTGCCACCCTCAA
AACCCTGGATGTCGCCGCGTTGCCGGGTGACCGTATCGAACTCAAGCTGTCGTTCGACGCACCGGTGCCAGTCCCGCGTG
GCTACACCACTGCGCAACCTGCGCGTATCGCGCTGGACCTGCCGGGTGTCACCAGCCAACTGGCGACCAAGAGTCGTGAA
CTGGGCACTGGCAACGCGCGCAGCGTTGTCGTGGTGCAGGCTCAGGATCGTACGCGGGTGATCATCAACCTGACGACGCT
GTCGCCTTATAGCTCGCGGGTCGACGGTAACAATCTGTTCGTGGTCATTGGTCAGGGCGCAACTGCGGCGCAGGCTTCCC
AGCCAAGCACCGCGCTGGGCGCATCCCGAGTGGCAGTGCCACCGCCCACGCCTGCTTCCGCCAGACCCTTTGTACCTGCC
GGTGCCAAGGCCATCAGGAATATCGACTTTCAGCGCGGCGAGTTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAACGC
CAACATTGCGCCTGACATTCAGGAGCAGGGCGGCAAAATTCGCGTGGACTTCGCCAAGACAATGCTGCCCGAGCCGTTGC
GCGTCCGGCTCGACGTCAAGGACTTCGCAACGCCGGTGCAGTTCGTCAGTGCTTCGGCCGCAGGCGACAAAGCGAGCATT
CTGATCGAGCCTTCGGGCGCATTCGACTATTCGGCGTTTCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCGCTGAC
CCACGAAGACATGGACCGGCGTGCTGCCGACAAACCGGTGTACACCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATTG
ATGTCCGCTCTGTGCTGCAACTGATCGCCGACTTCACCAACCTCAACCTGGTCGCCAGTGACACGGTTCAGGGCGGCATC
ACCTTGCGCCTGCAAAATGTGCCCTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGAT
AGGCAGCGTCCTGCTGGTCGCTCCGGCCGATGAAATTGCCGCCCGCGAACGTCAGGAACTGGAATCGCTCAAGCAGATTG
CCGAGCTGGCGCCGTTGCGTCGCGAGCTGTTGCAGGTCAACTACGCCAAGGCTGCTGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGACTGACGAGCGTGGCTCCATCACGGTCGATGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGACCGTCTCGACGAGCTGCGGCGCATCGTTTCGCAGCTGGATATTCCAGTACGTCAGGTGATGATCGAAGCGC
GCATCGTTGAGGCCAACGTCGATTACGACAAAGAGCTGGGGGTGCGCTGGGGTGGCGCGAGCAATACCAGCGGTGACAGC
AACTGGTCGACGGGCCGCAACAACTCAACGTTTGTCGATATGGGGGCTGCCAACGCCACCTCTGGAATCGGCCTTGGCTT
CCTGACCAACAATACGATGCTTGACCTTGAGCTGACTGCGATGGAAAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCA
AGGTGGTCACGTCCGACAAGGAGACCGCCAAGATCCTCAAGGGCACCGAGGTTCCGTATCAGGAATCCAGCTCCAGCGGC
GCCACCACGGTCAGCTTCAAGGAAGCGTCCCTGTCGCTTGAAGTCACCCCGCAGATCACGCCCGACAACCGCATCATCAT
GGAGGTCAAGGTCACCAAGGATGAGCCCGACTACGTGAATGCGGTGCTGGGTGTGCCGCCGATAAAGAAAAACGAAGTCA
ACGCCAAAGTGCTGATTTCCGACGGTGAAACCATCGTGATTGGTGGTGTGTTCTCGAATACGCAAAGTAAAAGTGTGGAT
AAAGTGCCATTTCTTGGCGATGTACCGTATCTTGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCT
GTTGGTATTTTTGACTCCGCGTATCATGAACAACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(520-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.944

100

0.756
  comQ Acinetobacter baylyi ADP1

41.847

100

0.423
  pilQ Acinetobacter baumannii strain A118

38.451

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.451

100

0.413
  pilQ Legionella pneumophila strain Lp02

37.189

99.708

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.189

99.708

0.371

Multiple sequence alignment