Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N018_RS01895 Genome accession   NZ_CP007014
Coordinates   406926..407990 (+) Length   354 a.a.
NCBI ID   WP_024646248.1    Uniprot ID   W0MKD8
Organism   Pseudomonas syringae CC1557     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401926..412990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N018_RS01885 (N018_01900) - 402787..404055 (+) 1269 WP_025388709.1 malic enzyme-like NAD(P)-binding protein -
  N018_RS01890 (N018_01905) - 404275..406716 (-) 2442 WP_418903500.1 penicillin-binding protein 1A -
  N018_RS01895 (N018_01910) pilM 406926..407990 (+) 1065 WP_024646248.1 pilus assembly protein PilM Machinery gene
  N018_RS01900 (N018_01915) - 407990..408565 (+) 576 WP_024646247.1 PilN domain-containing protein -
  N018_RS01905 (N018_01920) pilO 408562..409185 (+) 624 WP_024646246.1 type 4a pilus biogenesis protein PilO -
  N018_RS01910 (N018_01925) pilP 409182..409709 (+) 528 WP_024646245.1 type 4a pilus biogenesis lipoprotein PilP -
  N018_RS01915 (N018_01930) pilQ 409757..411814 (+) 2058 WP_024646244.1 type IV pilus secretin PilQ Machinery gene
  N018_RS01920 (N018_01935) aroK 411819..412337 (+) 519 WP_007248056.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38059.61 Da        Isoelectric Point: 4.7602

>NTDB_id=115711 N018_RS01895 WP_024646248.1 406926..407990(+) (pilM) [Pseudomonas syringae CC1557]
MFGLFNKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=115711 N018_RS01895 WP_024646248.1 406926..407990(+) (pilM) [Pseudomonas syringae CC1557]
GTGTTCGGACTCTTCAACAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTTGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCCTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGCCAGGCGTTGTCTCGCGTGCTGGTAAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTG
GCAGTGGCCGGTTCTGCGGTGATCACCAAAACCATCGAGATGGACGCCGGTCTTTCCGACGACGATATGGAAAACCAGCT
CAAGCTCGAAGCTGATCAGTACATTCCCTATCCACTTGAAGAAGTCGCCATCGATTTTGAAGTGCAGGGCTACTCGGTTC
GCAACCCCGAGCGTGTCGAGGTCCTGCTGGCGGCTTGCCGCAAGGAAAACGTCGAAGTACGCGAAGCTGCACTGGCGCTG
GCCGGGCTGACGGCGCGCGTCGTGGACGTTGAGGCCTATGCACTGGAGCGCTCATTTGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCACGACGAACTGACCGTTGCGGTGGTCGATATCGGCGCCACCATGACTACCCTGAGCGTGCTGCACCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTCGGCGGTCGCCAACTGACTGACGAGATCCAGCGTCGCTATGGCTTGTCCATG
GAAGAGGCCGGGCTCGCCAAGAAACAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTCGAGCCTTTCAAGGATGC
GCTGGTGCAACAGGTCTCACGCTCGTTGCAGTTCTTTTTTGCGGCCGGGCAATACAACTCGGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCTATTTCCGGACTGGAGCATCTGATCCAGCGACGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCTTGAGCGCCAAGGTCAATGCAGGAGCACTGGCCAGTGATGCACCGGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0MKD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472

Multiple sequence alignment