Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG32_RS09395 Genome accession   NZ_CP010132
Coordinates   1805977..1807362 (-) Length   461 a.a.
NCBI ID   WP_040090001.1    Uniprot ID   -
Organism   Escherichia coli strain C10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1800977..1812362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG32_RS09365 (RG32_08915) mutT 1801423..1801812 (+) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -
  RG32_RS09370 (RG32_08925) yacG 1801912..1802109 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RG32_RS09375 (RG32_08930) zapD 1802119..1802862 (-) 744 WP_001194734.1 cell division protein ZapD -
  RG32_RS09380 (RG32_08935) coaE 1802862..1803482 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  RG32_RS27080 - 1803507..1803551 (+) 45 WP_120795372.1 protein YacM -
  RG32_RS09385 (RG32_08940) guaC 1803707..1804750 (+) 1044 WP_040089999.1 GMP reductase -
  RG32_RS09390 (RG32_08945) hofC 1804785..1805987 (-) 1203 WP_000157234.1 protein transport protein HofC -
  RG32_RS09395 (RG32_08950) pilB 1805977..1807362 (-) 1386 WP_040090001.1 type II secretion system protein GspE Machinery gene
  RG32_RS09400 (RG32_08955) pilA 1807372..1807812 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  RG32_RS09410 (RG32_08965) nadC 1808015..1808908 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG32_RS09415 (RG32_08970) ampD 1808996..1809547 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG32_RS09420 (RG32_08975) ampE 1809544..1810398 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  RG32_RS09425 (RG32_08980) aroP 1810441..1811814 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50507.20 Da        Isoelectric Point: 6.4959

>NTDB_id=115679 RG32_RS09395 WP_040090001.1 1805977..1807362(-) (pilB) [Escherichia coli strain C10]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGDPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=115679 RG32_RS09395 WP_040090001.1 1805977..1807362(-) (pilB) [Escherichia coli strain C10]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGATCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.562

100

0.447
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Legionella pneumophila strain ERS1305867

50

82.863

0.414
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.891

83.731

0.393
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.831

88.72

0.362

Multiple sequence alignment