Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OM978_RS18410 Genome accession   NZ_OX352320
Coordinates   3917171..3918868 (+) Length   565 a.a.
NCBI ID   WP_264343768.1    Uniprot ID   -
Organism   Rheinheimera sp. MM224     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3912171..3923868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM978_RS18380 (JAMGFMIE_03697) ampE 3912791..3913678 (-) 888 WP_264343757.1 beta-lactamase regulator AmpE -
  OM978_RS18385 (JAMGFMIE_03698) ampD 3913679..3914227 (-) 549 WP_264343759.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OM978_RS18390 (JAMGFMIE_03699) - 3914305..3914823 (+) 519 WP_264343761.1 TIGR02281 family clan AA aspartic protease -
  OM978_RS18395 (JAMGFMIE_03700) nadC 3914872..3915741 (+) 870 WP_264343763.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OM978_RS18400 (JAMGFMIE_03701) - 3915858..3916508 (+) 651 WP_264343765.1 cold shock and DUF1294 domain-containing protein -
  OM978_RS18405 (JAMGFMIE_03702) - 3916703..3917080 (+) 378 WP_264343767.1 pilin -
  OM978_RS18410 (JAMGFMIE_03703) pilB 3917171..3918868 (+) 1698 WP_264343768.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM978_RS18415 (JAMGFMIE_03704) pilC 3918900..3920120 (+) 1221 WP_264343770.1 type II secretion system F family protein Machinery gene
  OM978_RS18420 (JAMGFMIE_03705) pilD 3920120..3921001 (+) 882 WP_264343771.1 prepilin peptidase Machinery gene
  OM978_RS18425 (JAMGFMIE_03706) coaE 3921024..3921647 (+) 624 WP_264343772.1 dephospho-CoA kinase -
  OM978_RS18430 (JAMGFMIE_03707) zapD 3921686..3922432 (+) 747 WP_264343774.1 cell division protein ZapD -
  OM978_RS18435 (JAMGFMIE_03708) yacG 3922549..3922779 (+) 231 WP_147903582.1 DNA gyrase inhibitor YacG -
  OM978_RS18440 (JAMGFMIE_03709) mutT 3922847..3923251 (-) 405 WP_264343778.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62105.53 Da        Isoelectric Point: 6.6235

>NTDB_id=1155318 OM978_RS18410 WP_264343768.1 3917171..3918868(+) (pilB) [Rheinheimera sp. MM224]
MALNPSSILLKRLQQANLVDPQITAKLLQEKSHQYQTIADLLIGEKLIQSKVLAEAAASFTLHSSMDLDFFDINAIPEQL
RNEKLIRKHHILPLSKRHKTLFLAVVDPTDMTSVEDFEFNTGLNAEMIVVEFDKLHKAIDKLFDTTATDSFTDTHWDLAK
MGLAGGEEEDTKAVGSDSEDQPIISFINKMLHDAIRKGASDLHFEPYEKSYRIRFRIDGILHEVASPPVALSTRLSARLK
VMSRLDIAEKRVPQDGRIKLKLSQKKSIDFRVSTLPTLWGEKIVMRILDSDSAMLGIDILGYEVEQKQLYLDALAQPQGM
ILVTGPTGSGKTVSLYTGLNILNTEETNISTAEDPVEINLPGINQVQVNPRAGLTFPSALKAFLRQDPDVIMVGEIRDIE
TAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVTLIIAQRLARRLCNHCKAAEQLPEQEALKQGFSAT
QLSQLKLFKPVGCDHCTGGYKGRLGIYEVMPITAQIAAKIMAGANSLDIAAQAQVEGFPNLRQSALKKAAAGFTSLAEVN
RVTNA

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1155318 OM978_RS18410 WP_264343768.1 3917171..3918868(+) (pilB) [Rheinheimera sp. MM224]
ATGGCATTAAACCCAAGCTCAATTTTACTAAAACGTTTGCAACAGGCGAATCTGGTCGACCCGCAGATCACCGCCAAACT
GCTGCAAGAAAAAAGTCACCAGTATCAAACTATTGCCGATTTGTTAATTGGCGAAAAACTGATTCAATCAAAAGTACTGG
CAGAAGCCGCAGCCTCTTTTACCTTACACAGCTCTATGGATTTAGACTTTTTTGATATCAATGCTATCCCGGAGCAACTA
CGTAACGAAAAACTGATTCGTAAACACCATATTCTGCCGCTGAGTAAAAGACATAAAACGCTGTTTCTTGCAGTGGTCGA
CCCGACGGATATGACCTCTGTAGAAGATTTTGAATTTAATACCGGCCTGAACGCCGAAATGATAGTGGTCGAGTTTGATA
AGCTGCACAAAGCGATCGACAAGCTCTTTGATACCACAGCTACTGACAGCTTTACCGATACGCATTGGGATTTGGCAAAA
ATGGGCCTGGCCGGTGGTGAAGAAGAAGACACCAAAGCAGTGGGTTCGGACAGTGAAGATCAGCCTATTATTTCTTTTAT
CAACAAAATGCTGCACGACGCTATTCGTAAAGGTGCTTCAGATCTGCATTTTGAGCCTTACGAAAAAAGCTACCGTATCC
GTTTTCGGATTGATGGTATTTTGCATGAAGTTGCATCACCACCTGTAGCTTTATCTACCCGCTTATCCGCCCGTTTAAAG
GTGATGTCCAGATTAGATATAGCCGAAAAACGTGTGCCTCAGGATGGCCGCATTAAATTAAAATTGTCTCAGAAAAAGTC
GATAGACTTTCGGGTCAGTACTTTGCCGACCTTGTGGGGCGAAAAAATAGTGATGCGGATCCTCGACTCCGACAGCGCTA
TGTTAGGTATTGATATTCTGGGTTACGAAGTAGAGCAAAAACAGTTGTATCTGGACGCCTTAGCCCAACCACAAGGCATG
ATTCTGGTGACAGGCCCGACGGGCTCAGGCAAAACCGTCTCTTTGTATACAGGGCTGAATATTCTCAATACCGAAGAAAC
CAATATATCCACAGCTGAAGACCCGGTTGAAATTAACCTGCCAGGTATCAATCAAGTGCAGGTCAATCCAAGAGCGGGAT
TGACCTTCCCTTCTGCGTTAAAAGCTTTTTTACGGCAAGACCCTGATGTCATTATGGTCGGTGAGATCCGTGATATTGAA
ACCGCTGAAATCGCGATTAAAGCGGCTCAAACCGGGCATTTAGTCTTAAGCACCTTGCACACAAACTCAGCGCCTGAAAC
CTTAACCCGCCTGCTGAATATGGGTGTACCAGCTTATAACGTCGCAAGCTCTGTCACCTTGATTATCGCTCAGCGCCTGG
CTCGGCGTTTATGCAACCATTGCAAAGCCGCAGAGCAACTGCCCGAACAGGAAGCGTTAAAACAAGGCTTTTCTGCCACG
CAGCTCAGCCAACTCAAACTGTTTAAGCCTGTGGGTTGTGATCATTGCACTGGCGGTTACAAAGGTCGTTTGGGCATTTA
TGAAGTCATGCCTATCACAGCGCAAATTGCGGCGAAAATTATGGCAGGAGCTAATTCGCTGGATATTGCCGCTCAGGCAC
AAGTGGAAGGATTCCCAAACTTACGCCAGTCGGCGTTAAAAAAAGCAGCAGCAGGCTTCACCAGTCTGGCGGAAGTGAAT
AGGGTGACCAACGCTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-147)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.674

99.823

0.556
  pilB Acinetobacter baumannii D1279779

56.093

98.761

0.554
  pilB Legionella pneumophila strain ERS1305867

56.357

93.274

0.526
  pilB Vibrio cholerae strain A1552

51.706

98.584

0.51
  pilB Vibrio campbellii strain DS40M4

50.722

98.053

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

52.533

94.336

0.496
  pilF Neisseria gonorrhoeae MS11

49.822

99.469

0.496
  pilB/pilB1 Synechocystis sp. PCC 6803

39.469

93.274

0.368

Multiple sequence alignment