Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OOL07_RS00475 Genome accession   NZ_OX241534
Coordinates   85707..87413 (-) Length   568 a.a.
NCBI ID   WP_264694104.1    Uniprot ID   -
Organism   Candidatus Nitrosacidococcus sp. I8 isolate NURINAE_1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 80707..92413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOL07_RS00440 (NURINAE_00088) - 81325..81555 (+) 231 WP_264694090.1 zinc-finger domain-containing protein -
  OOL07_RS00445 (NURINAE_00089) - 81566..81766 (-) 201 WP_264694092.1 DUF2905 domain-containing protein -
  OOL07_RS00450 (NURINAE_00090) - 81800..82762 (+) 963 WP_264694094.1 Nudix family hydrolase -
  OOL07_RS00455 (NURINAE_00091) yacG 82759..82971 (-) 213 WP_264694096.1 DNA gyrase inhibitor YacG -
  OOL07_RS00460 (NURINAE_00092) coaE 82978..83631 (-) 654 WP_264694098.1 dephospho-CoA kinase -
  OOL07_RS00465 (NURINAE_00093) pilD 83588..84457 (-) 870 WP_264694100.1 prepilin peptidase Machinery gene
  OOL07_RS00470 (NURINAE_00094) pilC 84485..85699 (-) 1215 WP_264694102.1 type II secretion system F family protein Machinery gene
  OOL07_RS00475 (NURINAE_00095) pilB 85707..87413 (-) 1707 WP_264694104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOL07_RS09240 lptM 87537..87671 (+) 135 WP_413774115.1 LPS translocon maturation chaperone LptM -
  OOL07_RS00480 (NURINAE_00096) lysA 87680..88930 (+) 1251 WP_264694106.1 diaminopimelate decarboxylase -
  OOL07_RS00485 (NURINAE_00097) - 88954..89622 (+) 669 WP_264694110.1 O-methyltransferase -
  OOL07_RS00490 (NURINAE_00098) dapF 89629..90471 (+) 843 WP_264694113.1 diaminopimelate epimerase -
  OOL07_RS00495 (NURINAE_00099) xerC 90642..91559 (+) 918 WP_264694116.1 tyrosine recombinase XerC -
  OOL07_RS00500 (NURINAE_00100) - 91755..92084 (+) 330 WP_264694118.1 c-type cytochrome -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62853.92 Da        Isoelectric Point: 6.2413

>NTDB_id=1154282 OOL07_RS00475 WP_264694104.1 85707..87413(-) (pilB) [Candidatus Nitrosacidococcus sp. I8 isolate NURINAE_1]
MTPSKTQIATLSHCLAAHNLLSESDALLYYKKSIQENISFISYLTQHKLVDSLTIAQIASQEFGIPLFSLNVLDISYSPQ
NLIEEKLIHEHRIFPLFKRGNKLFIAISDPTNQQALSNIKFYTGISIEPILVEEDKLSSAIDKINQGHGRALAELSSTLE
TVASTQNEDEVEENFIESDEEDAPIVRFVNRLLLDAINRGASDIHFEPYEKTYRVRYRQDGILHEVATPPVNLSPRITAR
IKVMARLDIAERRIPQDGRMKAKISDKKSIDFRVSTCPTLFGEKAVLRILDPTSAQIGIHSLGYEPEQQKIFLDTIHQSY
GMVLVTGPTGSGKTVSLYTALNILNTGNINISTAEDPAEINVPGINQVNVYPKVGLTFASALRSFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLVNMGVPSYNIASAVSLIIAQRLVRRLCTHCKTKEHIPDQVLLEEGFT
EGQIADRPIFYRANGCDHCAGGYKGRIGIYQVMPVSEIMGEIIMGGGNSMELAGQAKKEGIADLRQSGFKKIISGVTSLE
EVNRVTKE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1154282 OOL07_RS00475 WP_264694104.1 85707..87413(-) (pilB) [Candidatus Nitrosacidococcus sp. I8 isolate NURINAE_1]
ATGACACCATCAAAAACACAAATTGCCACCTTATCTCATTGCCTTGCAGCTCATAATCTATTAAGTGAATCAGATGCTCT
GCTCTATTATAAAAAATCTATACAAGAAAATATCTCTTTTATCTCCTATTTAACTCAGCACAAGCTAGTTGATAGCCTGA
CTATTGCCCAAATTGCTTCCCAAGAATTTGGAATACCGTTGTTTAGTTTAAATGTTTTAGATATTAGCTATAGCCCTCAA
AATCTCATTGAAGAAAAACTAATTCATGAGCATCGAATATTTCCTTTATTTAAACGGGGGAATAAGTTATTTATCGCAAT
TTCTGATCCTACTAACCAACAGGCACTCAGTAATATTAAATTTTATACTGGAATAAGCATTGAGCCTATCTTAGTAGAGG
AAGATAAGCTCTCTTCTGCAATTGATAAAATCAATCAAGGTCATGGAAGAGCACTAGCAGAACTCAGTAGCACTTTGGAA
ACCGTTGCGAGTACTCAAAATGAAGATGAGGTAGAAGAAAATTTTATTGAGTCAGACGAGGAAGACGCTCCTATAGTTCG
TTTTGTAAATCGTCTACTTCTTGATGCCATCAACCGTGGAGCTTCAGATATTCATTTTGAGCCTTATGAAAAAACCTATC
GGGTGCGTTATCGGCAAGATGGTATTCTACACGAGGTAGCTACTCCTCCGGTAAATTTATCCCCCCGAATTACTGCTCGA
ATTAAGGTAATGGCACGATTAGATATTGCAGAGCGGCGGATTCCTCAGGATGGGCGGATGAAAGCAAAAATTTCTGACAA
AAAATCCATTGATTTCCGAGTCAGTACTTGCCCTACCTTATTTGGAGAAAAGGCAGTACTTAGAATTTTAGATCCTACTA
GTGCCCAAATCGGTATTCACTCTTTAGGTTATGAACCAGAACAGCAAAAGATATTTTTAGATACTATCCATCAATCTTAC
GGCATGGTATTGGTTACTGGACCCACTGGAAGTGGTAAAACCGTTTCTCTTTACACCGCACTAAATATTCTCAACACGGG
GAATATAAATATTTCTACCGCTGAAGATCCTGCGGAAATCAACGTACCAGGGATTAATCAAGTCAATGTGTATCCCAAAG
TAGGACTTACTTTTGCCAGTGCTCTTAGATCTTTCCTACGTCAAGATCCTGATGTGATTATGGTAGGTGAGATTCGAGAT
CTAGAAACTGCAGAGATTGCCATTAAAGCAGCACAAACAGGGCATATGGTGCTCTCTACCCTACATACTAATGATGCCCC
TCAAACTCTAACTCGATTAGTTAATATGGGGGTACCTTCTTATAACATCGCCTCTGCTGTCTCCTTAATTATTGCGCAAC
GGTTAGTGCGTCGTTTATGCACTCATTGTAAAACAAAAGAGCATATCCCTGATCAGGTGCTCCTAGAAGAAGGGTTTACT
GAAGGGCAAATCGCAGATAGGCCTATTTTTTATAGAGCAAATGGTTGTGATCATTGTGCTGGAGGATATAAAGGTCGTAT
TGGTATCTATCAGGTGATGCCTGTCTCTGAAATCATGGGAGAAATAATTATGGGCGGAGGAAATTCAATGGAATTAGCAG
GTCAGGCAAAAAAGGAGGGTATTGCAGACTTACGCCAATCTGGATTTAAAAAAATTATTTCTGGAGTAACAAGCTTAGAG
GAAGTAAACCGAGTAACTAAAGAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.08

98.768

0.544
  pilB Acinetobacter baumannii D1279779

56.32

94.718

0.533
  pilB Acinetobacter baylyi ADP1

55.351

95.423

0.528
  pilF Neisseria gonorrhoeae MS11

54.102

90.141

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

47.88

99.648

0.477
  pilB Vibrio campbellii strain DS40M4

49.624

93.662

0.465
  pilB Vibrio cholerae strain A1552

50.775

90.845

0.461
  pilF Thermus thermophilus HB27

39.615

91.549

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.922

86.092

0.361

Multiple sequence alignment