Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   Q0V31_RS08000 Genome accession   NZ_OX044366
Coordinates   1694870..1696984 (+) Length   704 a.a.
NCBI ID   WP_298190975.1    Uniprot ID   -
Organism   MAG: uncultured Pseudomonas sp. isolate AG_MB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1689870..1701984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0V31_RS07980 pilM 1692040..1693104 (+) 1065 WP_298186566.1 pilus assembly protein PilM Machinery gene
  Q0V31_RS07985 - 1693104..1693676 (+) 573 WP_298186568.1 PilN domain-containing protein -
  Q0V31_RS07990 pilO 1693673..1694296 (+) 624 WP_298186570.1 type 4a pilus biogenesis protein PilO -
  Q0V31_RS07995 pilP 1694302..1694817 (+) 516 WP_298186572.1 type 4a pilus biogenesis lipoprotein PilP -
  Q0V31_RS08000 pilQ 1694870..1696984 (+) 2115 WP_298190975.1 type IV pilus secretin PilQ Machinery gene
  Q0V31_RS08005 aroK 1696989..1697507 (+) 519 WP_298186575.1 shikimate kinase AroK -
  Q0V31_RS08010 aroB 1697559..1698662 (+) 1104 WP_298186577.1 3-dehydroquinate synthase -
  Q0V31_RS08015 - 1698673..1700205 (+) 1533 WP_298186580.1 AAA family ATPase -

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 75569.78 Da        Isoelectric Point: 4.6763

>NTDB_id=1154228 Q0V31_RS08000 WP_298190975.1 1694870..1696984(+) (pilQ) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
MNISPSFLGFPLLAALLSPSLMAADLQALDVAALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDLPGVSNKLGAKTRE
LGVGNARSVTVVEAKDRTRLIINLTSLSPYNTRAEGNSVFVVVGEGGSNVASNAQPVAPAAAVAVTAKKYAAQSNAISNI
DFQRGELGEGNIVITLSDASVSPDIQDQGGKIRLDFAGTQLPESLRVKLDVKDFATPVQFVSATGTANKASILIEPVGVY
DYLAYQTDNKLTISVKPLTQDQAEQRKADSFSYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVP
WDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESKKQVADLAPLRRELIQVNYAKASDMAQLFQSVTSADGAA
TERGSVTVDDRTNSIIAYQTQERLDELRRIIAQLDVAVRQVMIEARIVEANVDYDKALGVRWGGSATRGNWSAYGKDGAQ
SFNDEGQRLLPGTDTIGGFTLEDGVAPVPFVDMGVLGSTSGIGIGFLTDNIVLDLQLSAMEKTGNGEIISQPKVVTSDKE
TAKILKGSEIPYQEASSSGATTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFARALNGVPPINKNEVNAKVLVSD
GETIVLGGVFSNTQTKGVEKVPFLGDLPYLGRLFRRDIVQDNKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=1154228 Q0V31_RS08000 WP_298190975.1 1694870..1696984(+) (pilQ) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
ATGAACATATCCCCATCTTTTTTAGGGTTTCCTCTTTTGGCTGCGCTTCTCTCGCCATCACTAATGGCCGCAGACCTGCA
GGCGCTTGACGTGGCTGCGTTGCCCGGAGACCGTGTTGAATTAAAATTAACTTTTGACGAGCCTGTGCTGGCGCCGCGGG
GCTACACCATTGAGCAGCCAGCGCGTATTGCGCTTGACTTGCCTGGGGTCTCTAACAAGCTAGGTGCCAAAACCAGAGAG
TTAGGCGTAGGTAATGCGCGCAGTGTTACCGTTGTTGAGGCGAAAGACCGTACGCGACTTATCATAAACTTGACCAGTCT
TTCTCCATATAACACTCGTGCAGAAGGGAATAGCGTTTTTGTGGTGGTGGGGGAAGGTGGTAGTAATGTTGCGTCTAATG
CTCAGCCAGTGGCGCCTGCAGCAGCAGTGGCCGTCACTGCAAAAAAATATGCAGCTCAGTCAAATGCGATCAGCAATATC
GACTTTCAGCGCGGAGAGTTGGGTGAGGGTAATATCGTAATTACTTTGTCCGACGCCTCTGTTAGCCCTGATATTCAGGA
TCAAGGCGGGAAAATCCGTTTGGACTTTGCCGGGACGCAGTTGCCAGAGTCGCTAAGGGTAAAGCTCGACGTAAAAGATT
TTGCCACGCCTGTTCAGTTTGTCAGTGCTACAGGTACAGCCAATAAAGCAAGCATTCTGATTGAGCCTGTGGGTGTTTAT
GATTATCTGGCTTACCAAACAGATAATAAGCTGACTATTAGTGTAAAGCCGTTGACCCAGGATCAGGCGGAGCAACGTAA
AGCCGATAGCTTCTCCTATACGGGTGAAAAGCTATCGCTCAACTTCCAAGATATTGATGTTCGTTCTGTCCTGCAGTTGA
TTGCAGACTTTACGGACCTCAACTTGGTTGCTAGTGATACGGTGGCTGGAAATATTACGTTGCGCCTGCAGAATGTTCCG
TGGGATCAAGCCTTGGATCTAGTGCTCAAGACCAAGGGGCTTGATAAGCGGAAGGTCGGTAATGTGCTGCTGGTTGCGCC
GGCTGACGAAATAGCGGCGCGTGAACGTCAAGAGCTTGAGTCAAAGAAGCAAGTTGCAGACCTGGCTCCTCTGCGCCGGG
AGCTTATTCAGGTCAATTATGCCAAGGCTTCCGATATGGCTCAGCTGTTCCAGTCCGTGACAAGCGCGGACGGTGCAGCA
ACTGAGCGCGGCTCGGTGACGGTTGATGATCGAACCAATAGTATTATTGCCTACCAAACACAAGAGCGGCTTGATGAGTT
GCGCCGGATTATCGCGCAGCTTGATGTGGCTGTGCGCCAAGTAATGATCGAAGCTCGTATTGTTGAGGCGAATGTCGATT
ACGATAAAGCTTTGGGTGTGCGTTGGGGTGGTTCTGCCACGCGTGGAAACTGGAGCGCGTATGGTAAAGACGGTGCTCAA
TCGTTTAATGATGAGGGGCAGCGGCTTTTGCCTGGTACGGACACCATTGGTGGTTTCACTCTAGAAGATGGCGTGGCGCC
TGTTCCGTTTGTAGATATGGGCGTGTTGGGGAGCACTTCGGGTATCGGTATTGGTTTCTTGACCGATAATATTGTTTTGG
ATCTTCAGTTGTCGGCGATGGAAAAAACCGGCAACGGTGAAATTATTTCGCAGCCGAAAGTTGTTACCTCTGATAAAGAG
ACGGCGAAGATCCTGAAGGGTTCAGAGATTCCTTATCAAGAGGCAAGCTCGAGTGGTGCTACAACGACCTCGTTTAAGGA
AGCCGCCTTGTCCCTTGAGGTGACGCCGCAAATCACTCCGGATAATCGAATTATCATGGAGGTCAAGGTCACTAAAGATG
CGCCTGATTTTGCCCGGGCCCTGAATGGTGTGCCGCCGATCAATAAGAACGAAGTTAATGCCAAGGTTCTTGTGAGCGAC
GGCGAGACAATTGTCCTCGGTGGGGTTTTCTCAAATACCCAAACTAAAGGTGTTGAGAAGGTACCTTTCCTTGGGGACCT
GCCTTACTTGGGGCGTTTGTTCCGTCGCGATATTGTTCAGGATAATAAGTCTGAACTCTTGGTATTCCTTACTCCGCGTA
TTATGAACAACCAAGCCATTGCGGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(539-695)

AMIN domain (AMIN)

(39-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.224

100

0.774
  comQ Acinetobacter baylyi ADP1

40.993

100

0.411
  pilQ Acinetobacter baumannii D1279779

40.714

99.432

0.405
  pilQ Acinetobacter baumannii strain A118

40.714

99.432

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.462

96.023

0.369
  pilQ Legionella pneumophila strain Lp02

38.314

96.023

0.368

Multiple sequence alignment