Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Q0V31_RS07980 Genome accession   NZ_OX044366
Coordinates   1692040..1693104 (+) Length   354 a.a.
NCBI ID   WP_298186566.1    Uniprot ID   -
Organism   MAG: uncultured Pseudomonas sp. isolate AG_MB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1687040..1698104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0V31_RS07970 - 1688079..1689347 (+) 1269 WP_298186560.1 malic enzyme-like NAD(P)-binding protein -
  Q0V31_RS07975 - 1689444..1691858 (-) 2415 WP_298186563.1 penicillin-binding protein 1A -
  Q0V31_RS07980 pilM 1692040..1693104 (+) 1065 WP_298186566.1 pilus assembly protein PilM Machinery gene
  Q0V31_RS07985 - 1693104..1693676 (+) 573 WP_298186568.1 PilN domain-containing protein -
  Q0V31_RS07990 pilO 1693673..1694296 (+) 624 WP_298186570.1 type 4a pilus biogenesis protein PilO -
  Q0V31_RS07995 pilP 1694302..1694817 (+) 516 WP_298186572.1 type 4a pilus biogenesis lipoprotein PilP -
  Q0V31_RS08000 pilQ 1694870..1696984 (+) 2115 WP_298190975.1 type IV pilus secretin PilQ Machinery gene
  Q0V31_RS08005 aroK 1696989..1697507 (+) 519 WP_298186575.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37903.23 Da        Isoelectric Point: 4.4263

>NTDB_id=1154227 Q0V31_RS07980 WP_298186566.1 1692040..1693104(+) (pilM) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIADLEGVGQALSRVLAKSKSGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGESARNADRVEVLLAACRKENVEVREAALAL
AGFTAKVVDVEAYALERAYSLLVDQLGGGRDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVARSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1154227 Q0V31_RS07980 WP_298186566.1 1692040..1693104(+) (pilM) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTACTCGGGATCGATATCAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGCCGCTCCGGAAGCCGCTACAAAGTAGAGGCTTACGCAGTCGAGCCGCTCCCGCCAAACGCTGTGGTCGAAAAGAACA
TTGCCGACCTAGAAGGCGTGGGGCAGGCCCTTTCACGTGTGCTCGCCAAATCCAAGAGCGGTGTAAAAACCGTTGCGGTG
GCTGTGGCCGGATCAGCAGTGATTACCAAAACTATCGAGATGGAAGCCGGGCTTTCTGACGATGAACTGGAAAATCAGCT
GAAGATCGAGGCCGATCAGTACATTCCTTATCCGTTGGAGGAGGTTGCGATCGATTTCGAGGTTCAGGGTGAATCTGCAC
GCAATGCTGATCGGGTTGAAGTGTTGCTTGCCGCTTGCCGCAAAGAGAATGTTGAGGTTCGCGAGGCTGCTTTAGCGCTT
GCTGGATTCACCGCCAAAGTGGTTGACGTTGAAGCCTATGCGCTTGAGCGTGCATACAGCTTGCTGGTTGATCAGCTTGG
CGGGGGGCGTGACGAGTTGACTGTGGCGGTTGTTGATATTGGCGCAACCATGACCACGCTCAGCGTCCTGCATAACGGTC
GGACTATCTACACCCGTGAGCAACTGTTTGGTGGCAAGCAACTCACCGAGGAAATTCAGCGTCGTTATGGGCTCTCTGTT
GAAGAGGCAGGTCTTGCCAAGAAGCAGGGCGGCTTGCCGGATGACTATGACAGCGAAGTACTGCAGCCATTTAAAGAGGC
TGTGGTTCAGCAGGTTGCTCGCTCCCTGCAATTCTTCTTCGCCGCGGGGCAGTTCAACGATGTCGATTACATCCTGCTAG
CGGGTGGTACGGCTTCAATTCCGGACCTTGATCGCTTGATCCAGCAAAAAATAGGCACCCAAACATTGGTCGCCAATCCA
TTCGCTGAGATGGCGCTGAGTGGCAAGGTTAATGCCGGTGCACTCGCCAGTGACGCACCGGCCTTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment