Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG28_RS04445 Genome accession   NZ_CP010122
Coordinates   850963..852348 (+) Length   461 a.a.
NCBI ID   WP_001025150.1    Uniprot ID   -
Organism   Escherichia coli strain C5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 845963..857348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG28_RS04415 (RG28_04190) aroP 846511..847884 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  RG28_RS04420 (RG28_04195) ampE 847927..848781 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RG28_RS04425 (RG28_04200) ampD 848778..849329 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG28_RS04430 (RG28_04205) nadC 849417..850310 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG28_RS04440 (RG28_04215) pilA 850513..850953 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RG28_RS04445 (RG28_04220) pilB 850963..852348 (+) 1386 WP_001025150.1 type II secretion system protein GspE Machinery gene
  RG28_RS04450 (RG28_04225) hofC 852338..853540 (+) 1203 WP_000157235.1 protein transport protein HofC -
  RG28_RS04455 (RG28_04230) guaC 853575..854618 (-) 1044 WP_073520857.1 GMP reductase -
  RG28_RS31715 - 854774..854818 (-) 45 WP_120795372.1 protein YacM -
  RG28_RS04460 (RG28_04235) coaE 854843..855463 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  RG28_RS04465 (RG28_04240) zapD 855463..856206 (+) 744 WP_001194734.1 cell division protein ZapD -
  RG28_RS04470 (RG28_04245) yacG 856216..856413 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RG28_RS04475 (RG28_04255) mutT 856513..856902 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50601.34 Da        Isoelectric Point: 6.6611

>NTDB_id=115408 RG28_RS04445 WP_001025150.1 850963..852348(+) (pilB) [Escherichia coli strain C5]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=115408 RG28_RS04445 WP_001025150.1 850963..852348(+) (pilB) [Escherichia coli strain C5]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.769

100

0.449
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.891

83.731

0.393
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.831

88.72

0.362

Multiple sequence alignment