Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG27_RS22295 Genome accession   NZ_CP010121
Coordinates   4331046..4332431 (+) Length   461 a.a.
NCBI ID   WP_001025197.1    Uniprot ID   -
Organism   Escherichia coli strain C4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4326046..4337431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG27_RS22265 (RG27_21195) aroP 4326594..4327967 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  RG27_RS22270 (RG27_21200) ampE 4328010..4328864 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  RG27_RS22275 (RG27_21205) ampD 4328861..4329412 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG27_RS22280 (RG27_21210) nadC 4329500..4330393 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RG27_RS22290 (RG27_21220) pilA 4330596..4331036 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RG27_RS22295 (RG27_21225) pilB 4331046..4332431 (+) 1386 WP_001025197.1 type II secretion system protein GspE Machinery gene
  RG27_RS22300 (RG27_21230) hofC 4332421..4333623 (+) 1203 WP_000157240.1 protein transport protein HofC -
  RG27_RS22305 (RG27_21235) guaC 4333658..4334701 (-) 1044 WP_001217337.1 GMP reductase -
  RG27_RS26580 - 4334857..4334901 (-) 45 WP_120795372.1 protein YacM -
  RG27_RS22310 (RG27_21240) coaE 4334926..4335546 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  RG27_RS22315 (RG27_21245) zapD 4335546..4336289 (+) 744 WP_001194720.1 cell division protein ZapD -
  RG27_RS22320 (RG27_21250) yacG 4336299..4336496 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RG27_RS22325 (RG27_21255) mutT 4336584..4336982 (-) 399 WP_000736038.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50738.50 Da        Isoelectric Point: 6.6256

>NTDB_id=115388 RG27_RS22295 WP_001025197.1 4331046..4332431(+) (pilB) [Escherichia coli strain C4]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQREPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLEMHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=115388 RG27_RS22295 WP_001025197.1 4331046..4332431(+) (pilB) [Escherichia coli strain C4]
ATGAATATTCCACAGCTTACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATTCCCATAGCCGGACTAAACCAGACGCAAATTCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTCTCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAAGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAATGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.052

83.514

0.401
  pilB Vibrio cholerae strain A1552

47.15

83.731

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

41.032

88.286

0.362

Multiple sequence alignment