Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F543_RS06790 Genome accession   NZ_CP006955
Coordinates   1453382..1453828 (+) Length   148 a.a.
NCBI ID   WP_025267127.1    Uniprot ID   A0A4V7IAF9
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-189     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1448382..1458828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F543_RS06770 (F543_14200) rnhA 1448486..1448944 (-) 459 WP_015432356.1 ribonuclease HI -
  F543_RS06775 (F543_14210) - 1449287..1449688 (-) 402 WP_015432355.1 H-NS family nucleoid-associated regulatory protein -
  F543_RS06780 (F543_14230) - 1450193..1451737 (+) 1545 WP_015432353.1 Na+/H+ antiporter NhaC family protein -
  F543_RS06785 (F543_14240) radA 1451828..1453207 (-) 1380 WP_015432352.1 DNA repair protein RadA Machinery gene
  F543_RS06790 (F543_14250) pilA 1453382..1453828 (+) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  F543_RS06795 (F543_14260) pilB 1453886..1455283 (+) 1398 WP_015432350.1 GspE/PulE family protein Machinery gene
  F543_RS12345 (F543_14270) pilC 1455276..1455779 (+) 504 WP_331279681.1 type II secretion system F family protein Machinery gene
  F543_RS12350 (F543_14280) pilC 1455878..1456471 (+) 594 WP_331279682.1 type II secretion system F family protein Machinery gene
  F543_RS06805 (F543_14290) - 1456468..1457133 (+) 666 WP_025328967.1 prepilin peptidase -
  F543_RS06810 (F543_14300) coaE 1457163..1457786 (+) 624 WP_015432347.1 dephospho-CoA kinase -
  F543_RS06815 (F543_14310) yacG 1457783..1457989 (+) 207 WP_015432346.1 DNA gyrase inhibitor YacG -
  F543_RS06820 (F543_14320) - 1458049..1458603 (-) 555 WP_015432345.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15806.99 Da        Isoelectric Point: 7.5807

>NTDB_id=115224 F543_RS06790 WP_025267127.1 1453382..1453828(+) (pilA) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
MKISNFRPLQKGFTLIELMIVIAIIAILATIAIPSYTNYTQKAALSEMLRASASYKSDVEICIYNTGATTNCSAGSNGIQ
AAKTESSDTKYLKSVSVTNGIINVEGKGSLDGYSYTMTPKFANNNITWSVNCTGADTGLFPANFCSSE

Nucleotide


Download         Length: 447 bp        

>NTDB_id=115224 F543_RS06790 WP_025267127.1 1453382..1453828(+) (pilA) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
ATGAAAATTTCTAACTTTCGCCCACTTCAAAAGGGCTTTACTTTAATTGAACTCATGATCGTGATTGCGATCATTGCAAT
TCTCGCTACTATCGCGATTCCATCTTATACCAACTATACTCAAAAAGCGGCTCTCTCTGAAATGTTACGTGCTTCTGCCT
CTTACAAATCAGATGTGGAAATTTGTATTTATAACACAGGGGCTACTACCAACTGCTCCGCAGGATCAAATGGAATTCAA
GCTGCGAAAACAGAGAGTAGTGATACAAAATATTTGAAATCAGTCTCTGTCACTAATGGCATTATTAATGTTGAAGGTAA
AGGCAGCCTTGATGGATATAGCTATACCATGACACCTAAATTTGCTAACAACAATATTACTTGGTCAGTCAATTGCACTG
GGGCAGACACAGGCTTATTCCCTGCTAATTTCTGTAGTTCGGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V7IAF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

71.141

100

0.716
  pilA Haemophilus influenzae 86-028NP

57.432

100

0.574
  pilA Haemophilus influenzae Rd KW20

56.081

100

0.561
  comP Acinetobacter baylyi ADP1

44.094

85.811

0.378
  pilA Vibrio campbellii strain DS40M4

38.028

95.946

0.365

Multiple sequence alignment