Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F543_RS06785 Genome accession   NZ_CP006955
Coordinates   1451828..1453207 (-) Length   459 a.a.
NCBI ID   WP_015432352.1    Uniprot ID   A0A4V7IAC4
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-189     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1446828..1458207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F543_RS06770 (F543_14200) rnhA 1448486..1448944 (-) 459 WP_015432356.1 ribonuclease HI -
  F543_RS06775 (F543_14210) - 1449287..1449688 (-) 402 WP_015432355.1 H-NS family nucleoid-associated regulatory protein -
  F543_RS06780 (F543_14230) - 1450193..1451737 (+) 1545 WP_015432353.1 Na+/H+ antiporter NhaC family protein -
  F543_RS06785 (F543_14240) radA 1451828..1453207 (-) 1380 WP_015432352.1 DNA repair protein RadA Machinery gene
  F543_RS06790 (F543_14250) pilA 1453382..1453828 (+) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  F543_RS06795 (F543_14260) pilB 1453886..1455283 (+) 1398 WP_015432350.1 GspE/PulE family protein Machinery gene
  F543_RS12345 (F543_14270) pilC 1455276..1455779 (+) 504 WP_331279681.1 type II secretion system F family protein Machinery gene
  F543_RS12350 (F543_14280) pilC 1455878..1456471 (+) 594 WP_331279682.1 type II secretion system F family protein Machinery gene
  F543_RS06805 (F543_14290) - 1456468..1457133 (+) 666 WP_025328967.1 prepilin peptidase -
  F543_RS06810 (F543_14300) coaE 1457163..1457786 (+) 624 WP_015432347.1 dephospho-CoA kinase -
  F543_RS06815 (F543_14310) yacG 1457783..1457989 (+) 207 WP_015432346.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49502.19 Da        Isoelectric Point: 8.3945

>NTDB_id=115223 F543_RS06785 WP_015432352.1 1451828..1453207(-) (radA) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
MAKAPKTAYVCSDCGEEYSKWMGQCRACLAWNTISEVRLISSKQESKGARFSGYAGQTSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLSSRLPTLYVTGEESLQQVAMRANRLGLPTEHLKMLSETSVEH
ICNLADQEKPKLMVIDSIQVMHLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDASL
LLEGEADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSEEQTSGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLEQNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLALLSSFRNRPLPHDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIVPAGNIPKKSIKGMEVLGVKKLSDALDIVANL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=115223 F543_RS06785 WP_015432352.1 1451828..1453207(-) (radA) [Bibersteinia trehalosi USDA-ARS-USMARC-189]
ATGGCAAAAGCACCGAAAACCGCTTATGTATGTAGTGATTGTGGCGAAGAGTATTCAAAATGGATGGGGCAATGTCGTGC
TTGTTTAGCATGGAATACGATTAGTGAAGTACGGTTAATTTCGTCAAAACAAGAAAGCAAAGGTGCTCGCTTTAGTGGCT
ACGCGGGGCAAACTTCTGGTAAGGTACAAACGCTTTCAGAGATCAGCTTGCAGGAAGTACCAAGGTTTAGCAGTGGCTTT
AATGAACTAGATCGAGTATTAGGCGGAGGGATTGTACCTGGAAGTGCAATTTTAATTGGCGGGCATCCCGGGGCAGGAAA
AAGCACTTTATTACTCCAAGTTATGTGTGGGTTGTCATCAAGATTGCCAACACTCTATGTAACAGGTGAAGAATCATTAC
AACAGGTTGCCATGCGAGCAAATCGTTTGGGGTTGCCAACCGAGCATTTGAAGATGCTTTCAGAAACCTCTGTTGAGCAT
ATTTGTAATCTTGCAGATCAGGAAAAACCCAAATTGATGGTTATTGATTCTATTCAGGTCATGCATCTTGCTGATATTCA
ATCTTCTCCAGGGAGTGTAGCACAGGTGCGAGAATGTGCTTCTTTTTTAACTCGTTATGCGAAAACCCGTCAAGTAGCGA
TTATTATGGTTGGACATGTGACGAAAGATGGCACATTAGCTGGTCCAAAAGTGTTAGAGCATGCCATTGATGCATCATTA
TTGTTAGAAGGGGAGGCAGATAGTCGCTTTAGAACCTTGCGTAGCCATAAGAACCGTTTTGGTGCAGTGAATGAACTCGG
TGTGTTTGCTATGACTGAGCAAGGTTTACGTGAAGTGAAAAATCCCTCAGCGATTTTCTTAAGTCGAAGTGAAGAGCAAA
CTTCTGGCAGTTCAGTTATGGTGCTTTGGGAAGGAACACGTCCATTATTAGTGGAAATTCAGGCATTAGTTGATCACTCT
ATGCTTGCCAATCCTCGCCGAGTTGCAGTGGGATTAGAGCAGAACCGTTTATCTCTATTACTAGCTGTTTTGCATCGCCA
TGGTGGCTTACAAATGTCCGATCAAGATGTGTTTGTCAATGTCGTTGGCGGAGTAAAAGTGACTGAAACTAGTGCAGATT
TAGCCCTGCTGTTAGCCTTGCTTTCTAGTTTTCGTAACCGACCATTGCCTCATGATCTGGTGGTTTTTGGTGAAGTAGGG
CTAGCAGGTGAAATCCGCCCAGTACCAAGTGGACAAGAGCGTATTAGTGAAGCCGCAAAACATGGATTTAAGCGCGCGAT
TGTCCCTGCTGGAAACATACCAAAGAAATCAATTAAGGGGATGGAAGTGCTTGGTGTGAAAAAGCTAAGTGATGCGTTGG
ACATTGTTGCGAATCTTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-224)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V7IAC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

99.346

0.484
  radA Streptococcus mitis NCTC 12261

44.13

100

0.442
  radA Streptococcus mitis SK321

43.723

100

0.44
  radA Streptococcus pneumoniae TIGR4

43.764

99.564

0.436
  radA Streptococcus pneumoniae D39

43.764

99.564

0.436
  radA Streptococcus pneumoniae Rx1

43.764

99.564

0.436
  radA Streptococcus pneumoniae R6

43.764

99.564

0.436

Multiple sequence alignment