Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KOD61_RS11120 Genome accession   NZ_OU015430
Coordinates   2363569..2365299 (+) Length   576 a.a.
NCBI ID   WP_215218729.1    Uniprot ID   -
Organism   Novilysobacter luteus strain CECT 30171     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2358569..2370299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOD61_RS11110 (LYB30171_02217) pilR 2361289..2362689 (+) 1401 WP_215218727.1 sigma-54 dependent transcriptional regulator Regulator
  KOD61_RS13095 (LYB30171_02218) pilA/pilA1 2362985..2363413 (+) 429 WP_215218728.1 pilin Machinery gene
  KOD61_RS11120 (LYB30171_02219) pilB 2363569..2365299 (+) 1731 WP_215218729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KOD61_RS11125 (LYB30171_02220) pilC 2365559..2366818 (+) 1260 WP_215218730.1 type II secretion system F family protein Machinery gene
  KOD61_RS11130 (LYB30171_02221) - 2366844..2367707 (+) 864 WP_215218731.1 A24 family peptidase -
  KOD61_RS11135 (LYB30171_02222) coaE 2367934..2368548 (+) 615 WP_215218732.1 dephospho-CoA kinase -
  KOD61_RS11140 (LYB30171_02223) - 2368650..2369684 (-) 1035 WP_215218733.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62962.15 Da        Isoelectric Point: 5.3424

>NTDB_id=1151000 KOD61_RS11120 WP_215218729.1 2363569..2365299(+) (pilB) [Novilysobacter luteus strain CECT 30171]
MNTATYANLVGITGLARRLVMDGALDEAKAREAMAGASAEKKPIATYLTEYRMASPSQLAAANSVEFGVPLFDPAAMDPE
QAAVTLVKEELLRKHCVLPLYKRGNRLFVGISEPTNTQALDEIKFQTNLSVEGVLVDEESIRRHIDRWLESADTLGDSMG
GDDEGLDTLEVGSDEDVAGGDAGVDAKTDDTPVVKFINKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQAARVPVK
LQQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDSSAAKLGIEKLGYEDDQRELY
LAAIAKPYGMVLVTGPTGSGKTVSLYTALNILNDETRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIGIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTLVVAQRLARRLHACKREVHLPEHA
LLAEGFTAEEIASPDFHIYEAVGCPDCTEGYKGRTGIYQVMPMSDEIQEIVLAGGNAMQIADAAQKAGIRDLRQSALMKV
RNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=1151000 KOD61_RS11120 WP_215218729.1 2363569..2365299(+) (pilB) [Novilysobacter luteus strain CECT 30171]
ATGAATACCGCCACATATGCCAACCTCGTAGGAATCACTGGTCTCGCCCGCAGACTCGTCATGGACGGCGCGCTTGACGA
GGCAAAGGCACGAGAGGCAATGGCTGGTGCGTCGGCGGAAAAGAAACCCATCGCGACGTACCTCACCGAGTATCGGATGG
CGTCGCCATCCCAACTCGCGGCAGCAAACTCGGTCGAGTTCGGCGTGCCTTTGTTCGATCCCGCGGCAATGGACCCTGAG
CAGGCCGCGGTCACGCTGGTTAAAGAAGAGCTTCTCCGCAAGCATTGCGTGCTACCGCTGTACAAGCGTGGCAACCGGCT
GTTCGTCGGCATCAGTGAACCGACCAATACCCAGGCACTGGATGAGATCAAATTCCAGACCAATCTTTCGGTCGAAGGCG
TTCTGGTCGATGAAGAAAGCATCCGTCGCCACATCGACCGCTGGTTGGAGTCGGCCGACACCCTCGGCGACAGCATGGGC
GGGGACGACGAGGGGCTGGACACGCTGGAGGTCGGCAGCGACGAAGACGTCGCAGGCGGCGACGCAGGCGTCGACGCCAA
GACCGACGACACCCCGGTCGTCAAGTTCATCAACAAGGTGCTGGTCGACGCTATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAGACCGAGTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGCGGCCAGGGTGCCGGTGAAG
CTGCAGCAGCGCATCGCCGCGCGCTTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTCAGCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTATTCGGCGAGAAGATCG
TGCTACGCATCCTCGACAGCAGCGCCGCCAAGCTCGGCATCGAAAAACTCGGCTACGAGGACGACCAGCGCGAACTGTAC
CTGGCCGCGATCGCGAAGCCCTACGGCATGGTGCTTGTGACCGGCCCCACCGGCTCGGGTAAGACCGTGTCGCTGTACAC
CGCACTGAACATCCTCAACGACGAGACCCGCAACATCTCCACGGTCGAAGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ATCAAGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTACGCAGCTTCCTGCGCCAGGACCCGGACGTG
ATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGGCATCAAGGCCGCACAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCATTCAACATCACGTCGT
CGGTGACCCTGGTGGTGGCCCAGCGCCTGGCGCGCCGGCTGCACGCCTGCAAGCGCGAGGTGCACCTGCCCGAGCACGCT
CTGCTGGCGGAAGGGTTCACCGCGGAGGAGATCGCCAGTCCGGACTTCCACATCTACGAGGCCGTGGGCTGCCCGGACTG
TACCGAGGGCTACAAGGGCCGGACCGGCATCTACCAGGTGATGCCGATGAGCGACGAGATCCAGGAGATCGTGCTGGCCG
GAGGCAACGCGATGCAGATTGCGGATGCCGCCCAGAAGGCCGGGATCCGTGACCTGCGGCAGTCGGCGTTGATGAAAGTA
CGCAACGGGGTGACAAGCTTGGCAGAGATCAACCGCGTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.83

98.264

0.549
  pilB Acinetobacter baylyi ADP1

55.203

98.438

0.543
  pilB Legionella pneumophila strain ERS1305867

50.791

98.785

0.502
  pilB Vibrio cholerae strain A1552

51.013

94.271

0.481
  pilF Neisseria gonorrhoeae MS11

48.336

99.132

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

50.566

92.014

0.465
  pilB Vibrio campbellii strain DS40M4

51.703

86.632

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.248

100

0.385

Multiple sequence alignment