Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   DTQ61_RS02720 Genome accession   NZ_LT985192
Coordinates   530482..532587 (+) Length   701 a.a.
NCBI ID   WP_060402733.1    Uniprot ID   A0A2K4X1K8
Organism   Pseudomonas syringae strain CFBP 2116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525482..537587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTQ61_RS02700 (PSCFBP2116_00493) pilM 527627..528691 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  DTQ61_RS02705 (PSCFBP2116_00494) - 528691..529290 (+) 600 WP_060402732.1 PilN domain-containing protein -
  DTQ61_RS02710 (PSCFBP2116_00495) pilO 529287..529910 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  DTQ61_RS02715 (PSCFBP2116_00496) pilP 529907..530434 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  DTQ61_RS02720 (PSCFBP2116_00497) pilQ 530482..532587 (+) 2106 WP_060402733.1 type IV pilus secretin PilQ Machinery gene
  DTQ61_RS02725 (PSCFBP2116_00498) aroK 532592..533110 (+) 519 WP_002551657.1 shikimate kinase AroK -
  DTQ61_RS02730 (PSCFBP2116_00499) aroB 533226..534329 (+) 1104 WP_060402734.1 3-dehydroquinate synthase -
  DTQ61_RS02735 (PSCFBP2116_00500) - 534340..535953 (+) 1614 WP_060402735.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75513.88 Da        Isoelectric Point: 5.0976

>NTDB_id=1148947 DTQ61_RS02720 WP_060402733.1 530482..532587(+) (pilQ) [Pseudomonas syringae strain CFBP 2116]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGNGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVIIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VASAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEASNTGSNVTSNVPFVDMGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNALLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1148947 DTQ61_RS02720 WP_060402733.1 530482..532587(+) (pilQ) [Pseudomonas syringae strain CFBP 2116]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTAAA
GACTCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAGCTCAAGCTGGCATTCGACTCGCCGGTGCCTGCGCCGCGCG
GTTACACCACGGCGCAACCTGCGCGTATTGCGCTGGACCTGCCGGGCGTGACCAGTCAGTTGGCGACCAAAAGCCGTGAA
CTCGGTAACGGTAACGCACGCAGCGTGGTCGTTGTACAGGCTCAGGACCGCACGCGGGTAATCATCAACCTGACCACGCT
TTCGCCCTACAGCTCCCGCGTCGACGGCAACAACCTTTACGTGGTTATCGGTCAGGGCGCAGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCACCCACTCCGGCATCTGCCAGACCGTTTATCCCCGCG
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGATCATAGATTTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTCGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTGCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCGTCGGCTACGGGCGATAAGGCCAGTATC
CTGATCGAACCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGGCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTACGCTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTAGCCAGCGACACGGTTCAGGGCGGTATT
ACCCTGCGTCTGCAGAACGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAGCGTCAGGAGCTTGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAATTGTTGCAGGTCAATTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGGCCAGCGCCGAGTCCAAGACCGACGAGCGTGGCTCGATCACTGTGGACGACCGGACCAACAACATCATTGCCTATCA
AACCCAGGACCGGCTCGACGAACTGCGTCGCATCGTCTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTGGGTGTGCGCTGGGGCGGCTCGACCAACACCAGCGGCAATGGA
AAATGGACAACCTATGGCCTTGATAATAATGGCGATGAGGCAAGTAACACTGGCAGCAACGTGACCTCCAACGTGCCGTT
TGTCGATATGGGCGCATCTGGCGCAACCTCCGGCATTGGCCTGGGCTTCGTCACCAACAATACGTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGTAACGGTGAAATCGTCTCCCAACCCAAGGTAGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACCGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCAACCACTGTGAGCTTCAAGGAGGCCTCGCT
GTCGCTTGAGGTAACGCCACAGATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGTTGCTTGGTGTACCACCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTCATTGGTGGTGTGTTCTCAAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Secretin and TonB N terminus short domain (STN)

(309-355)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K4X1K8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.646

100

0.739
  comQ Acinetobacter baylyi ADP1

40.463

100

0.424
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.405
  pilQ Legionella pneumophila strain Lp02

38.495

98.573

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.406

98.431

0.378

Multiple sequence alignment