Detailed information NTG1148946

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: DTQ61_RS02700
• Genome accession: NZ_LT985192
• Coordinates: 527627..528691 (+)
• Length: 354 a.a.
• NCBI ID: WP_002551652.1
• Uniprot ID: A0A7Z6Y2Y4
• Organism: Pseudomonas syringae strain CFBP 2116
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 522627..533691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DTQ61_RS02680 (PSCFBP2116_00491)	-	522825..524093 (+)	1269	WP_002551650.1	malic enzyme-like NAD(P)-binding protein	-
DTQ61_RS02695 (PSCFBP2116_00492)	-	524976..527417 (-)	2442	WP_375233967.1	penicillin-binding protein 1A	-
DTQ61_RS02700 (PSCFBP2116_00493)	pilM	527627..528691 (+)	1065	WP_002551652.1	pilus assembly protein PilM	Machinery gene
DTQ61_RS02705 (PSCFBP2116_00494)	-	528691..529290 (+)	600	WP_060402732.1	PilN domain-containing protein	-
DTQ61_RS02710 (PSCFBP2116_00495)	pilO	529287..529910 (+)	624	WP_002551654.1	type 4a pilus biogenesis protein PilO	-
DTQ61_RS02715 (PSCFBP2116_00496)	pilP	529907..530434 (+)	528	WP_002551655.1	type 4a pilus biogenesis lipoprotein PilP	-
DTQ61_RS02720 (PSCFBP2116_00497)	pilQ	530482..532587 (+)	2106	WP_060402733.1	type IV pilus secretin PilQ	Machinery gene
DTQ61_RS02725 (PSCFBP2116_00498)	aroK	532592..533110 (+)	519	WP_002551657.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38078.57 Da
• Isoelectric Point: 4.7008

>NTDB_id=1148946 DTQ61_RS02700 WP_002551652.1 527627..528691(+) (pilM) [Pseudomonas syringae strain CFBP 2116]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1148946 DTQ61_RS02700 WP_002551652.1 527627..528691(+) (pilM) [Pseudomonas syringae strain CFBP 2116]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTGCTTGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCGGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAATGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACAATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCTCGTTCTTTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A7Z6Y2Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469