Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C6H38_RS02860 Genome accession   NZ_LT963402
Coordinates   545952..548057 (+) Length   701 a.a.
NCBI ID   WP_060412076.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. avii isolate CFBP3846     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540952..553057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H38_RS02840 (CFBP3846_00528) pilM 543058..544122 (+) 1065 WP_060412078.1 pilus assembly protein PilM Machinery gene
  C6H38_RS02845 (CFBP3846_00529) - 544122..544760 (+) 639 WP_060412077.1 PilN domain-containing protein -
  C6H38_RS02850 (CFBP3846_00530) pilO 544757..545380 (+) 624 WP_005765501.1 type 4a pilus biogenesis protein PilO -
  C6H38_RS02855 (CFBP3846_00531) pilP 545377..545904 (+) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  C6H38_RS02860 (CFBP3846_00532) pilQ 545952..548057 (+) 2106 WP_060412076.1 type IV pilus secretin PilQ Machinery gene
  C6H38_RS02865 (CFBP3846_00533) aroK 548062..548580 (+) 519 WP_007245596.1 shikimate kinase AroK -
  C6H38_RS02870 (CFBP3846_00534) aroB 548699..549802 (+) 1104 WP_005765495.1 3-dehydroquinate synthase -
  C6H38_RS02875 (CFBP3846_00535) - 549813..551438 (+) 1626 WP_005765493.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75612.05 Da        Isoelectric Point: 4.9772

>NTDB_id=1148809 C6H38_RS02860 WP_060412076.1 545952..548057(+) (pilQ) [Pseudomonas syringae pv. avii isolate CFBP3846]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPAPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNDDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSRNG
KWTTYGLDDNGDEGGNTNANLTSNIPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1148809 C6H38_RS02860 WP_060412076.1 545952..548057(+) (pilQ) [Pseudomonas syringae pv. avii isolate CFBP3846]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGACCGCATCGAGCTCAAGCTTGCCTTTGACGCGCCGGTGCCTGCGCCTCGCG
GCTACACCACCGCGCAACCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGCTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCTCAGGATCGCACGCGAGTGATCATCAACCTGACGACGCT
TGCGCCTTACAGCTCTCGCGTTGACGGCAACAATCTGTTTGTGGTCATTGGCCAGGGTGCCGCCGCAGCGCAGGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCGCCAGCCCCGGCGTCTGCCAGACCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGTGGTGAGCTGGGCGAAGGCAATGTGGTCATAGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAACAGGGCGGCAAAATCCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGATGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCGTTGAC
CAACGACGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ATGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCCTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGTGTTCTGCTGGTTGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCACCGCTGCGCCGCGAGCTGTTGCAGGTCAATTACGCCAAGGCCGCCGATATCGCCAAGCTGTTTCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTGGACGATCGCACCAACAACATCATTGCCTACCA
GACTCAGGACCGTCTCGACGAACTGCGTCGCATTGTTTCGCAACTGGATATTCCAGTGCGCCAGGTGATGATCGAGGCGC
GTATCGTCGAGGCCAACGTCGATTACAACAAGCAGTTGGGTGTGCGCTGGGGTGGCTCGACCAATACCAGTCGCAACGGT
AAATGGACCACCTACGGTCTGGACGACAATGGTGATGAGGGCGGTAACACGAATGCAAACCTGACCTCCAACATCCCTTT
TGTTGACCTGGGTGCAGCCGATGCGACCACAGGCATTGGCTTGGGCTTCGTAACCAACAACACGCTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACAGGCAACGGTGAAATCGTCTCCCAGCCCAAAGTGGTCACATCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACAGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACCGTGAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATCACGCCGGACAACCGCATCATTATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCCATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.337

100

0.746
  comQ Acinetobacter baylyi ADP1

41.667

99.287

0.414
  pilQ Acinetobacter baumannii strain A118

38.556

100

0.404
  pilQ Acinetobacter baumannii D1279779

38.556

100

0.404
  pilQ Legionella pneumophila strain Lp02

37.807

98.859

0.374
  pilQ Legionella pneumophila strain ERS1305867

37.807

98.859

0.374

Multiple sequence alignment