Detailed information NTG1148808

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: C6H38_RS02840
• Genome accession: NZ_LT963402
• Coordinates: 543058..544122 (+)
• Length: 354 a.a.
• NCBI ID: WP_060412078.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. avii isolate CFBP3846
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 538058..549122

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H38_RS02825 (CFBP3846_00526)	-	538798..540066 (+)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-
C6H38_RS02835 (CFBP3846_00527)	-	540407..542848 (-)	2442	WP_415224734.1	penicillin-binding protein 1A	-
C6H38_RS02840 (CFBP3846_00528)	pilM	543058..544122 (+)	1065	WP_060412078.1	pilus assembly protein PilM	Machinery gene
C6H38_RS02845 (CFBP3846_00529)	-	544122..544760 (+)	639	WP_060412077.1	PilN domain-containing protein	-
C6H38_RS02850 (CFBP3846_00530)	pilO	544757..545380 (+)	624	WP_005765501.1	type 4a pilus biogenesis protein PilO	-
C6H38_RS02855 (CFBP3846_00531)	pilP	545377..545904 (+)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
C6H38_RS02860 (CFBP3846_00532)	pilQ	545952..548057 (+)	2106	WP_060412076.1	type IV pilus secretin PilQ	Machinery gene
C6H38_RS02865 (CFBP3846_00533)	aroK	548062..548580 (+)	519	WP_007245596.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38076.60 Da
• Isoelectric Point: 4.7008

>NTDB_id=1148808 C6H38_RS02840 WP_060412078.1 543058..544122(+) (pilM) [Pseudomonas syringae pv. avii isolate CFBP3846]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMESQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1148808 C6H38_RS02840 WP_060412078.1 543058..544122(+) (pilM) [Pseudomonas syringae pv. avii isolate CFBP3846]
GTGTTCGAACTCTTTGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATCGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCTGGTCTCTCCGACGACGACATGGAAAGTCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCTTACCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAAGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACCGTTGCCGTGGTTGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCATGGTC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAGGTGCTGGACCCCTTCAAGGACGC
GCTGGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCGTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACACCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGTTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCACCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469