Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C6H34_RS25205 Genome accession   NZ_LT963395
Coordinates   5420426..5422531 (-) Length   701 a.a.
NCBI ID   WP_003432146.1    Uniprot ID   A0A193SWW1
Organism   Pseudomonas cerasi isolate PL963     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5415426..5427531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H34_RS25190 (PL963_04927) - 5417051..5418673 (-) 1623 WP_058397764.1 SPOR domain-containing protein -
  C6H34_RS25195 (PL963_04928) aroB 5418684..5419787 (-) 1104 WP_065350824.1 3-dehydroquinate synthase -
  C6H34_RS25200 (PL963_04929) aroK 5419903..5420421 (-) 519 WP_003365999.1 shikimate kinase AroK -
  C6H34_RS25205 (PL963_04930) pilQ 5420426..5422531 (-) 2106 WP_003432146.1 type IV pilus secretin PilQ Machinery gene
  C6H34_RS25210 (PL963_04931) pilP 5422579..5423106 (-) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  C6H34_RS25215 (PL963_04932) pilO 5423103..5423726 (-) 624 WP_003432147.1 type 4a pilus biogenesis protein PilO -
  C6H34_RS25220 (PL963_04933) - 5423723..5424298 (-) 576 WP_003365994.1 PilN domain-containing protein -
  C6H34_RS25225 (PL963_04934) pilM 5424298..5425362 (-) 1065 WP_003365992.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75331.66 Da        Isoelectric Point: 5.0294

>NTDB_id=1148799 C6H34_RS25205 WP_003432146.1 5420426..5422531(-) (pilQ) [Pseudomonas cerasi isolate PL963]
MTRILSIIGVSLCIAVLAPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTGSSTTSNVPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1148799 C6H34_RS25205 WP_003432146.1 5420426..5422531(-) (pilQ) [Pseudomonas cerasi isolate PL963]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGGTGCTGGCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCGGCACCGCGCG
GTTACACCACGTCGCAACCAGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGTTGGCGACCAAAAGTCGTGAC
CTGGGCACCGGTAATGCGCGCAGTGTGGTTGTGGTACAGGCTCAGGACCGCACCCGGGTGATCATCAACCTGACCACGCT
TTCGCCGTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCTTCCC
AACCGACCACCGCAATGGGTGCGCAACGGGTCGCGGTCCCTCCGCCCACGCCTGCATCCGCCAAACCGTTCATTCCCGCA
GGCGTCAAGGCCATCCGCAATGTCGACTTCCAGCGTGGCGAGCTGGGTGAGGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGC
GCGTCCGGCTGGACGTCAAGGACTTCGCCACGCCGGTACAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
TTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGTGTGCGGCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCCGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTTAACTTCCAGGACATTG
ATGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGCCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGACGAGCGCGGTTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGAGCGCCTCGACGAGCTGCGTCGTATCGTGTCGCAACTGGATATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTCGGGGTTCGCTGGGGCGGTTCTACCAACACCAGCGGCAGCGGC
AAATGGACAACCTACGGGCTGGATAATAACGGTGACGAGGCCGGTAACACTGGCAGCAGTACTACCTCCAACGTGCCGTT
CGTGGACCTGGGCGCCGCTGATGCGACGACCGGTATCGGCCTGGGCTTCGTGACCAATAACACGCTGCTGGACCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCCTCCCT
GTCGCTTGAGGTAACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTTACCAAGGACGAGCCGGACT
ACCTGAATGCGGTGCTGGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193SWW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.194

100

0.752
  comQ Acinetobacter baylyi ADP1

42.241

99.287

0.419
  pilQ Acinetobacter baumannii D1279779

39.456

100

0.414
  pilQ Acinetobacter baumannii strain A118

39.456

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.551

98.431

0.379
  pilQ Legionella pneumophila strain Lp02

38.551

98.431

0.379

Multiple sequence alignment