Detailed information NTG1148800

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: C6H34_RS25225
• Genome accession: NZ_LT963395
• Coordinates: 5424298..5425362 (-)
• Length: 354 a.a.
• NCBI ID: WP_003365992.1
• Uniprot ID: A0A193SY06
• Organism: Pseudomonas cerasi isolate PL963
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5419298..5430362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H34_RS25200 (PL963_04929)	aroK	5419903..5420421 (-)	519	WP_003365999.1	shikimate kinase AroK	-
C6H34_RS25205 (PL963_04930)	pilQ	5420426..5422531 (-)	2106	WP_003432146.1	type IV pilus secretin PilQ	Machinery gene
C6H34_RS25210 (PL963_04931)	pilP	5422579..5423106 (-)	528	WP_003350151.1	type 4a pilus biogenesis lipoprotein PilP	-
C6H34_RS25215 (PL963_04932)	pilO	5423103..5423726 (-)	624	WP_003432147.1	type 4a pilus biogenesis protein PilO	-
C6H34_RS25220 (PL963_04933)	-	5423723..5424298 (-)	576	WP_003365994.1	PilN domain-containing protein	-
C6H34_RS25225 (PL963_04934)	pilM	5424298..5425362 (-)	1065	WP_003365992.1	pilus assembly protein PilM	Machinery gene
C6H34_RS25230 (PL963_04935)	-	5425572..5428013 (+)	2442	WP_414860623.1	penicillin-binding protein 1A	-
C6H34_RS25235 (PL963_04936)	-	5428741..5430009 (-)	1269	WP_003365988.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38104.65 Da
• Isoelectric Point: 4.7049

>NTDB_id=1148800 C6H34_RS25225 WP_003365992.1 5424298..5425362(-) (pilM) [Pseudomonas cerasi isolate PL963]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1148800 C6H34_RS25225 WP_003365992.1 5424298..5425362(-) (pilM) [Pseudomonas cerasi isolate PL963]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCCTTGCCGGCCAACGCCGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAGGCTGATCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTTGAAGTGCGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCGCGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGCTCTTTCGGGCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCACGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAAGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGACGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCGCCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCGCCAGCCCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A193SY06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	53.955	100	0.54
pilM	Legionella pneumophila strain ERS1305867	46.479	100	0.466