Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CKV84_RS05045 Genome accession   NZ_LT906452
Coordinates   1072922..1075018 (+) Length   698 a.a.
NCBI ID   WP_027220941.1    Uniprot ID   -
Organism   Legionella pneumophila strain NCTC11985     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1067922..1080018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV84_RS05020 (SAMEA4040028_00984) ravI 1068684..1069691 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  CKV84_RS05025 (SAMEA4040028_00985) pilM 1070108..1071172 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  CKV84_RS05030 (SAMEA4040028_00986) pilN 1071176..1071724 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  CKV84_RS05035 (SAMEA4040028_00987) pilO 1071738..1072337 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  CKV84_RS05040 (SAMEA4040028_00988) pilP 1072334..1072918 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  CKV84_RS05045 (SAMEA4040028_00989) pilQ 1072922..1075018 (+) 2097 WP_027220941.1 type IV pilus secretin PilQ Machinery gene
  CKV84_RS05050 (SAMEA4040028_00990) aroK 1075464..1075991 (+) 528 WP_010946667.1 shikimate kinase AroK -
  CKV84_RS05055 (SAMEA4040028_00991) aroB 1075978..1077087 (+) 1110 WP_027220942.1 3-dehydroquinate synthase -
  CKV84_RS05060 (SAMEA4040028_00992) - 1077084..1078535 (+) 1452 WP_027220943.1 SPOR domain-containing protein -
  CKV84_RS05065 (SAMEA4040028_00993) - 1078644..1079075 (+) 432 WP_011213360.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77455.73 Da        Isoelectric Point: 9.5345

>NTDB_id=1147428 CKV84_RS05045 WP_027220941.1 1072922..1075018(+) (pilQ) [Legionella pneumophila strain NCTC11985]
MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=1147428 CKV84_RS05045 WP_027220941.1 1072922..1075018(+) (pilQ) [Legionella pneumophila strain NCTC11985]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGCCTGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCCGAAAACAGAGTACGTATTGATTTTCAATTTTCCAAGCCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCGCCTCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCCTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATATTGGAACTGGA
TCGTTCAGTGTCCTATTCTGGCAGTACGGCTGGTTCTGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAGGGAGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAACTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAAAAGGTTCGCATGACCATCCTCAGCAAAGGGGATTATGGACATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGGGCTGTGCTTCAACTGCTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGCGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTT
GATATCATTTTAACCACACAAGGGTTAGATAAGCGCAGAAAGGGCAATGTCATGTTGATAGATACCAAAGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGATCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAACTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGTGGTACACTGGAAGGTGCTAACGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAGTTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACAAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCAGTATTGAGTTTGAAAGTTACGCCACAAATTACGCCTGA
TGGTAAAATTCTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGAGGTATTTATAAGCAAGATAAAAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAGA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)

AMIN domain (AMIN)

(39-113)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

99.857

100

0.999
  pilQ Legionella pneumophila strain Lp02

99.713

100

0.997
  pilQ Pseudomonas aeruginosa PAK

37.872

100

0.383
  comQ Acinetobacter baylyi ADP1

38.574

98.424

0.38

Multiple sequence alignment