Detailed information NTG1147424

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CKV84_RS05025
• Genome accession: NZ_LT906452
• Coordinates: 1070108..1071172 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain NCTC11985
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1065108..1076172

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV84_RS05015 (SAMEA4040028_00983)	-	1066148..1068532 (-)	2385	WP_027220940.1	penicillin-binding protein 1A	-
CKV84_RS05020 (SAMEA4040028_00984)	ravI	1068684..1069691 (-)	1008	WP_011946020.1	Dot/Icm T4SS effector RavI	-
CKV84_RS05025 (SAMEA4040028_00985)	pilM	1070108..1071172 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
CKV84_RS05030 (SAMEA4040028_00986)	pilN	1071176..1071724 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
CKV84_RS05035 (SAMEA4040028_00987)	pilO	1071738..1072337 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
CKV84_RS05040 (SAMEA4040028_00988)	pilP	1072334..1072918 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
CKV84_RS05045 (SAMEA4040028_00989)	pilQ	1072922..1075018 (+)	2097	WP_027220941.1	type IV pilus secretin PilQ	Machinery gene
CKV84_RS05050 (SAMEA4040028_00990)	aroK	1075464..1075991 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1147424 CKV84_RS05025 WP_011215094.1 1070108..1071172(+) (pilM) [Legionella pneumophila strain NCTC11985]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1147424 CKV84_RS05025 WP_011215094.1 1070108..1071172(+) (pilM) [Legionella pneumophila strain NCTC11985]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407