Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKV78_RS01740 Genome accession   NZ_LT906448
Coordinates   360996..362393 (+) Length   465 a.a.
NCBI ID   WP_005764663.1    Uniprot ID   -
Organism   Pasteurella dagmatis strain NCTC11617     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355996..367393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV78_RS01710 (SAMEA4362423_00373) - 356166..357017 (-) 852 WP_005764670.1 thymidylate synthase -
  CKV78_RS01715 (SAMEA4362423_00374) lgt 357023..357826 (-) 804 WP_005764669.1 prolipoprotein diacylglyceryl transferase -
  CKV78_RS01720 (SAMEA4362423_00375) - 357836..358627 (-) 792 WP_005764668.1 sulfite exporter TauE/SafE family protein -
  CKV78_RS01725 (SAMEA4362423_00376) rppH 358630..359229 (-) 600 WP_005764667.1 RNA pyrophosphohydrolase -
  CKV78_RS01730 (SAMEA4362423_00377) ampD 359846..360403 (-) 558 WP_005764666.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CKV78_RS01735 (SAMEA4362423_00378) pilA 360568..360996 (+) 429 WP_005764664.1 prepilin peptidase-dependent pilin Machinery gene
  CKV78_RS01740 (SAMEA4362423_00379) pilB 360996..362393 (+) 1398 WP_005764663.1 GspE/PulE family protein Machinery gene
  CKV78_RS01745 (SAMEA4362423_00380) pilC 362393..363607 (+) 1215 WP_005764662.1 type II secretion system F family protein Machinery gene
  CKV78_RS01750 (SAMEA4362423_00381) - 363604..364305 (+) 702 WP_005764661.1 A24 family peptidase -
  CKV78_RS01755 (SAMEA4362423_00382) coaE 364399..365010 (+) 612 WP_005764660.1 dephospho-CoA kinase -
  CKV78_RS01760 (SAMEA4362423_00383) yacG 365010..365213 (+) 204 WP_005764659.1 DNA gyrase inhibitor YacG -
  CKV78_RS01765 (SAMEA4362423_00384) - 365213..365482 (+) 270 WP_005764658.1 GNAT family N-acetyltransferase -
  CKV78_RS01770 (SAMEA4362423_00386) - 365881..367254 (+) 1374 WP_005764657.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52933.52 Da        Isoelectric Point: 4.8781

>NTDB_id=1147359 CKV78_RS01740 WP_005764663.1 360996..362393(+) (pilB) [Pasteurella dagmatis strain NCTC11617]
MFSQRNFTLIAKNGETFEITTDLWQRNQQQQAILLRYFALPLKEDERVLWLGVDSFANVSACETFSFLMGKIVEPVLLHS
SDLKSSLQQLSPQSLQVEESTEFVYQPQLTEISLQQEEQDEPVIQVLNDLFEQALQKNASDIHIEPQKQKLQIRFRIDGV
LQLQANLALTFSMRLVSRLKLLAKLDISEMRLPQDGRFQFKTRFSDILDFRLSTLPTYFGEKAVLRLQQNTPIDFSFADL
GMNAEQQIRFQNALNQPQGLILVTGPTGSGKSISLYTALQWLNSQEKHIMTAEDPIEIEVEGIIQTQINPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAQSSIARLKQLGIQQYEIENSLLLVIAQRLVRKLCKK
CDQDNLGSCDCYQGYAGRIGVYQLLYPKIKGHFQTDFATLRSSALEKVEQGLTDEMEVNRVLGSE

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=1147359 CKV78_RS01740 WP_005764663.1 360996..362393(+) (pilB) [Pasteurella dagmatis strain NCTC11617]
ATGTTTTCTCAACGTAATTTTACGCTGATTGCAAAGAATGGTGAAACTTTTGAGATCACTACTGATTTATGGCAACGCAA
TCAGCAACAACAAGCCATATTGCTACGTTACTTTGCATTGCCGCTAAAAGAAGATGAGCGTGTTTTATGGCTTGGTGTGG
ATTCCTTTGCCAATGTATCTGCTTGTGAAACGTTTTCCTTTTTGATGGGAAAAATTGTTGAACCCGTGTTATTACATTCT
TCGGATCTAAAAAGTTCTTTACAGCAACTATCTCCACAAAGTTTACAAGTAGAAGAAAGTACAGAGTTTGTTTATCAGCC
ACAATTAACGGAGATTTCTTTACAACAAGAGGAGCAAGATGAACCCGTCATTCAGGTCTTAAATGATCTGTTTGAGCAAG
CATTGCAGAAAAATGCTTCTGATATTCATATTGAGCCACAAAAACAAAAATTACAGATCCGTTTTCGTATTGATGGTGTT
TTACAACTACAAGCGAATCTTGCTTTAACTTTTTCTATGCGGCTGGTTTCTCGTTTAAAGCTGTTGGCTAAATTAGATAT
TAGCGAAATGCGTTTACCTCAAGATGGACGTTTTCAATTTAAAACGCGCTTTTCTGACATATTGGATTTTCGTTTGTCTA
CCTTGCCTACTTATTTTGGTGAGAAAGCAGTGTTGCGTTTACAGCAAAATACGCCAATAGATTTTAGTTTTGCTGATTTA
GGAATGAATGCGGAGCAGCAAATTCGTTTTCAAAATGCACTAAATCAACCACAGGGATTAATTTTAGTGACAGGTCCTAC
AGGGAGTGGGAAAAGTATTTCACTTTATACTGCACTTCAATGGTTAAATTCACAAGAAAAACATATTATGACCGCAGAGG
ATCCTATAGAAATTGAAGTTGAAGGAATTATCCAAACACAAATCAACCCACAAATTGGGCTCGATTTTAGCCGTTTATTA
CGAACTTTCTTACGTCAAGATCCGGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCTGCAATGGCATTGCG
TGCTGCACAAACTGGACATTTGGTGCTTTCGACATTACATACAAATGATGCGCAAAGTTCGATTGCCCGCTTAAAACAAT
TAGGAATTCAGCAATATGAAATTGAAAATAGCTTGTTACTGGTAATTGCACAGCGGTTAGTTCGGAAATTATGTAAGAAA
TGTGATCAAGATAATCTGGGAAGTTGTGATTGTTATCAAGGCTATGCTGGGCGTATAGGTGTTTATCAATTGCTTTATCC
TAAAATAAAAGGGCACTTCCAAACAGATTTTGCCACATTACGTAGTAGTGCTTTAGAAAAAGTTGAGCAAGGGCTGACGG
ATGAAATGGAAGTCAATCGTGTATTAGGAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-93)

Type II/IV secretion system protein (T2SSE)

(130-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

71.648

97.849

0.701
  pilB Haemophilus influenzae Rd KW20

71.648

97.849

0.701
  pilB Glaesserella parasuis strain SC1401

60.706

97.419

0.591
  pilB Vibrio parahaemolyticus RIMD 2210633

40.919

100

0.422
  pilB Acinetobacter baumannii D1279779

39.235

100

0.419
  pilB Vibrio campbellii strain DS40M4

40.501

100

0.417
  pilB Legionella pneumophila strain ERS1305867

39.388

100

0.415
  pilB Vibrio cholerae strain A1552

39.712

100

0.415
  pilB Acinetobacter baylyi ADP1

37.778

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.008

100

0.376

Multiple sequence alignment