Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CKV78_RS01735 Genome accession   NZ_LT906448
Coordinates   360568..360996 (+) Length   142 a.a.
NCBI ID   WP_005764664.1    Uniprot ID   C9PQ21
Organism   Pasteurella dagmatis strain NCTC11617     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355568..365996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV78_RS01705 (SAMEA4362423_00372) tadA 355639..356166 (-) 528 WP_032855631.1 tRNA adenosine(34) deaminase TadA -
  CKV78_RS01710 (SAMEA4362423_00373) - 356166..357017 (-) 852 WP_005764670.1 thymidylate synthase -
  CKV78_RS01715 (SAMEA4362423_00374) lgt 357023..357826 (-) 804 WP_005764669.1 prolipoprotein diacylglyceryl transferase -
  CKV78_RS01720 (SAMEA4362423_00375) - 357836..358627 (-) 792 WP_005764668.1 sulfite exporter TauE/SafE family protein -
  CKV78_RS01725 (SAMEA4362423_00376) rppH 358630..359229 (-) 600 WP_005764667.1 RNA pyrophosphohydrolase -
  CKV78_RS01730 (SAMEA4362423_00377) ampD 359846..360403 (-) 558 WP_005764666.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CKV78_RS01735 (SAMEA4362423_00378) pilA 360568..360996 (+) 429 WP_005764664.1 prepilin peptidase-dependent pilin Machinery gene
  CKV78_RS01740 (SAMEA4362423_00379) pilB 360996..362393 (+) 1398 WP_005764663.1 GspE/PulE family protein Machinery gene
  CKV78_RS01745 (SAMEA4362423_00380) pilC 362393..363607 (+) 1215 WP_005764662.1 type II secretion system F family protein Machinery gene
  CKV78_RS01750 (SAMEA4362423_00381) - 363604..364305 (+) 702 WP_005764661.1 A24 family peptidase -
  CKV78_RS01755 (SAMEA4362423_00382) coaE 364399..365010 (+) 612 WP_005764660.1 dephospho-CoA kinase -
  CKV78_RS01760 (SAMEA4362423_00383) yacG 365010..365213 (+) 204 WP_005764659.1 DNA gyrase inhibitor YacG -
  CKV78_RS01765 (SAMEA4362423_00384) - 365213..365482 (+) 270 WP_005764658.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15048.22 Da        Isoelectric Point: 7.0778

>NTDB_id=1147358 CKV78_RS01735 WP_005764664.1 360568..360996(+) (pilA) [Pasteurella dagmatis strain NCTC11617]
MKQRISIQKGFTLIELMIVIAIVAVLATIAIPSYQNYTKKAAISELLQAADPYRAEVELCIYNTGAKASCDAGTNGIQAS
HNTGKKYLKSIDVADGVITVTGQNALDKVSYTLTPSGDVTQGVSWQASCGTHSELFPAGFCS

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1147358 CKV78_RS01735 WP_005764664.1 360568..360996(+) (pilA) [Pasteurella dagmatis strain NCTC11617]
ATGAAACAACGTATTTCAATTCAAAAAGGATTTACTTTAATTGAACTTATGATTGTGATTGCTATTGTTGCTGTATTAGC
GACTATTGCAATTCCATCGTACCAAAATTATACCAAAAAAGCGGCAATTTCAGAATTGCTTCAAGCAGCAGATCCTTATC
GAGCAGAGGTTGAGCTGTGTATTTACAACACTGGTGCTAAGGCTAGCTGTGATGCAGGAACAAATGGTATTCAAGCCTCC
CATAATACAGGTAAAAAGTATCTAAAATCGATTGATGTAGCAGATGGTGTGATCACTGTCACTGGACAAAATGCATTAGA
TAAAGTCTCTTATACTTTAACTCCATCAGGAGATGTTACTCAAGGTGTTTCCTGGCAAGCAAGTTGTGGTACTCATTCTG
AGCTCTTTCCTGCTGGATTTTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C9PQ21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

57.664

96.479

0.556
  pilA Haemophilus influenzae Rd KW20

57.664

96.479

0.556
  pilA Glaesserella parasuis strain SC1401

54.286

98.592

0.535

Multiple sequence alignment