Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CFBP1590_RS24890 Genome accession   NZ_LT855380
Coordinates   5553980..5556079 (-) Length   699 a.a.
NCBI ID   WP_057410477.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain CFBP 1590 isolate E12-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5548980..5561079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP1590_RS24875 (CFBP1590__4966) - 5550590..5552218 (-) 1629 WP_088236114.1 SPOR domain-containing protein -
  CFBP1590_RS24880 (CFBP1590__4967) aroB 5552228..5553331 (-) 1104 WP_004882713.1 3-dehydroquinate synthase -
  CFBP1590_RS24885 (CFBP1590__4968) aroK 5553457..5553975 (-) 519 WP_004882714.1 shikimate kinase AroK -
  CFBP1590_RS24890 (CFBP1590__4969) pilQ 5553980..5556079 (-) 2100 WP_057410477.1 type IV pilus secretin PilQ Machinery gene
  CFBP1590_RS24895 (CFBP1590__4970) - 5556127..5556654 (-) 528 WP_029243168.1 pilus assembly protein PilP -
  CFBP1590_RS24900 (CFBP1590__4971) pilO 5556651..5557274 (-) 624 WP_088236115.1 type 4a pilus biogenesis protein PilO -
  CFBP1590_RS24905 (CFBP1590__4972) - 5557271..5557849 (-) 579 WP_025993896.1 PilN domain-containing protein -
  CFBP1590_RS24910 (CFBP1590__4973) pilM 5557849..5558913 (-) 1065 WP_004882719.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75358.75 Da        Isoelectric Point: 4.9910

>NTDB_id=1146542 CFBP1590_RS24890 WP_057410477.1 5553980..5556079(-) (pilQ) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
MTRILSIIGVSLWIAMMSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPTTAKGAARVAVPPPTPASARPYVPA
GVRAIRNVDFQRGELGEGNVVIDLSNASIAADIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVSATASGDKASI
SIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKQIGVRWGGRTDRSRKW
SVGGLDDNGDEAGNTGNDLTANIPFVDLGAPDATAGVGIGFLTNNALLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDVVAEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=1146542 CFBP1590_RS24890 WP_057410477.1 5553980..5556079(-) (pilQ) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
ATGACCCGGATTCTCTCGATTATCGGCGTTTCGTTATGGATAGCGATGATGTCGCCGGTTCTCCAGGCTGCCAATCTCAA
GACGCTCGACGTCGCAGCCCTGCCGGGTGATCGAATCGAGCTCAAGCTTTCCTTCGATGCACCGGTGCCCGCGCCACGCG
GCTATACCACCGAGCAGCCTGCGCGCATTGCGCTGGACCTGCCGGGCGTGACCAGCCAGCTGGCGACCAAGAGCCGTGAT
CTGGGCGCAGGCAATGCCCGCAGTGTGGTGGTTGTGCAGGCGCAGGACCGTACGCGAGTCATCATCAACCTGACGACGCT
TTCGCCGTACAGCTCACGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGTCAGGGTGCGGCGGCGGCACAGGCTTCGC
AGCCGACCACCGCCAAGGGCGCTGCACGGGTCGCCGTGCCGCCACCCACACCGGCATCGGCGCGGCCCTATGTGCCTGCG
GGCGTCAGAGCCATTCGCAATGTCGACTTCCAGCGCGGTGAGCTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAATGC
CAGCATCGCTGCAGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGATTTCGCCAAGACGCAATTGCCCGATCCGTTGC
GCGTGCGTCTGGACGTGAAAGACTTTGCCACACCCGTGCAATTCGTCAGCGCCACGGCGTCCGGCGACAAGGCCAGCATT
TCCATCGAGCCATCGGGTGCGTTCGATTATTCGGCGTTCCAGACCGAGAACAAGCTGACCATCAGCGTGCGCCCATTGAC
CAATGAGGACGTGGATCGTCGCGCTGCTGACAAGCCGGTCTACACCGGCGAGAAGCTTTCGCTGAATTTTCAGGACATCG
ATGTGCGTTCGGTACTGCAACTGATCGCCGATTTCACTAACCTCAATCTGGTCGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGTCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGT
CGGCAGCGTGTTGCTGGTCGCCCCGGCTGACGAGATCGCCGCGCGTGAGCGTCAGGAACTGGAGTCGCTCAAGCAGATTG
CCGAACTGGCCCCGTTGCGTCGTGAGCTGCTGCAGGTCAACTACGCCAAGGCTGCGGATATCGCCAAGCTGTTCCAGTCG
GTCACCAGCGCCGAGTCCAAGGCGGACGAGCGTGGTTCCATTACTGTGGATGAGCGCACCAACAACATCATTGCGTATCA
GACTCAGGATCGACTCGACGAGCTGCGCCGCATCGTCTCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGC
GGATAGTTGAAGCCAACGTCGACTATGACAAGCAGATCGGCGTGCGCTGGGGCGGGCGAACCGACCGGTCGCGCAAGTGG
AGTGTCGGCGGCCTCGACGATAATGGTGATGAAGCAGGCAACACGGGCAACGATCTGACGGCCAACATTCCGTTTGTGGA
TCTGGGTGCTCCAGATGCCACGGCGGGTGTCGGGATCGGTTTCCTGACCAACAATGCACTGCTGGACCTTGAGCTGAGTG
CTATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAGACTGCCAAGATCCTC
AAGGGCACGGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCCACGACAGTGAGCTTCAAGGAGGCGTCCCTGTCGCT
TGAGGTCACGCCACAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTTACCAAGGACGAGCCTGATTACCTCA
ATGCGGTGCTGGGCGTGCCTCCTATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGATGGCGAGACCATCGTG
ATCGGCGGGGTGTTCTCCAATACGCAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCAGGCGCGACGTGGTGGCAGAGGCGAAATCCGAGCTGTTGGTATTTCTGACACCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.399

100

0.747
  comQ Acinetobacter baylyi ADP1

40.656

100

0.408
  pilQ Acinetobacter baumannii D1279779

37.756

100

0.395
  pilQ Acinetobacter baumannii strain A118

37.756

100

0.395
  pilQ Legionella pneumophila strain Lp02

38.067

97.711

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.067

97.711

0.372

Multiple sequence alignment