Detailed information NTG1146543

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CFBP1590_RS24910
• Genome accession: NZ_LT855380
• Coordinates: 5557849..5558913 (-)
• Length: 354 a.a.
• NCBI ID: WP_004882719.1
• Uniprot ID: A0A0D0MR84
• Organism: Pseudomonas viridiflava strain CFBP 1590 isolate E12-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5552849..5563913

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFBP1590_RS24885 (CFBP1590__4968)	aroK	5553457..5553975 (-)	519	WP_004882714.1	shikimate kinase AroK	-
CFBP1590_RS24890 (CFBP1590__4969)	pilQ	5553980..5556079 (-)	2100	WP_057410477.1	type IV pilus secretin PilQ	Machinery gene
CFBP1590_RS24895 (CFBP1590__4970)	-	5556127..5556654 (-)	528	WP_029243168.1	pilus assembly protein PilP	-
CFBP1590_RS24900 (CFBP1590__4971)	pilO	5556651..5557274 (-)	624	WP_088236115.1	type 4a pilus biogenesis protein PilO	-
CFBP1590_RS24905 (CFBP1590__4972)	-	5557271..5557849 (-)	579	WP_025993896.1	PilN domain-containing protein	-
CFBP1590_RS24910 (CFBP1590__4973)	pilM	5557849..5558913 (-)	1065	WP_004882719.1	pilus assembly protein PilM	Machinery gene
CFBP1590_RS24915 (CFBP1590__4974)	-	5559123..5561564 (+)	2442	WP_374729078.1	penicillin-binding protein 1A	-
CFBP1590_RS24920 (CFBP1590__4975)	-	5561911..5562366 (+)	456	WP_029243170.1	YkgJ family cysteine cluster protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38031.45 Da
• Isoelectric Point: 4.7090

>NTDB_id=1146543 CFBP1590_RS24910 WP_004882719.1 5557849..5558913(-) (pilM) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVVGKAKTSARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSLRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1146543 CFBP1590_RS24910 WP_004882719.1 5557849..5558913(-) (pilM) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGTTCCACCTCGGTCAAGCTCCTTGAATT
GAGTCGTTCCGGAACCCGCTACAAAGTCGAGTCTTACGCTGTTGAGCCGTTGCCGGCCAATGCCGTCGTCGAGAAGAACA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCACGGGTCGTTGGCAAGGCCAAGACCAGCGCCAGAATAGTGGCGGTC
GCTGTCGCAGGCTCCGCAGTCATCACCAAGACGATCGAAATGGATGCCGGTCTCTCGGACGACGACATGGAAAATCAGCT
CAAGCTCGAGGCTGACCAGTACATCCCCTATCCCCTTGAAGAAGTGGCCATCGATTTCGAAGTGCAGGGCTATTCGCTGC
GCAACCCCGAACGCGTGGAAGTCCTTCTGGCTGCCTGTCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCGCTG
GCGGGCCTCACCGCAAAAGTTGTCGACGTCGAAGCCTATGCCCTTGAGCGTTCGTTCGGGCTGCTGGCTGCGCAACTGGG
TAACGGTCACGATGAGCTGACCGTGGCGGTCGTCGACATCGGCGCCACCATGACCACACTGAGCGTCCTGCACCATGGCC
GGATCATTTATACCCGTGAGCAACTGTTCGGCGGGCGCCAACTGACCGAAGAAATCCAGCGCCGCTATGGCCTGTCGATG
GAAGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCGGACGACTATGTCAGTGAAGTGCTGGAACCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCGCGCTCACTTCAGTTCTTCTTCGCGGCAGGCCAGTACAACTCTGTGGATCACATCATGCTCG
CTGGCGGTACGGCTTCCATTTCCGGCCTGGAGCATCTGATTCAGCGCCGACTCGGCACGCCGACCCAGGTGGCCAACCCG
TTTGCCGACATGGCCCTCAGTTCCAAGGTCAACGCCGGAGCGCTGGCCAGTGATGCGCCCGCGCTGATGATCGCGTGCGG
TCTTGCTTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0D0MR84

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.78	100	0.568
comM	Acinetobacter nosocomialis M2	56.78	100	0.568
comM	Acinetobacter baylyi ADP1	55.085	100	0.551
pilM	Legionella pneumophila strain ERS1305867	47.887	100	0.48