Detailed information NTG1144867

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: LLA12_RS06445
• Genome accession: NZ_LT599049
• Coordinates: 1343913..1344761 (+)
• Length: 282 a.a.
• NCBI ID: WP_021721698.1
• Uniprot ID: S6FQS2
• Organism: Lactococcus lactis subsp. lactis strain A12
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1338913..1349761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLA12_RS06420 (LLA12_01358)	ilvN	1339667..1340143 (+)	477	WP_010905839.1	acetolactate synthase small subunit	-
LLA12_RS06425 (LLA12_01359)	ilvC	1340189..1341211 (+)	1023	WP_021721696.1	ketol-acid reductoisomerase	-
LLA12_RS06430 (LLA12_01360)	ilvA	1341267..1342517 (+)	1251	WP_010905840.1	threonine ammonia-lyase IlvA	-
LLA12_RS06435 (LLA12_01361)	budA	1342636..1343346 (+)	711	WP_003131140.1	acetolactate decarboxylase	-
LLA12_RS06440 (LLA12_01362)	-	1343387..1343767 (+)	381	WP_068872817.1	RidA family protein	-
LLA12_RS06445 (LLA12_01363)	dprA	1343913..1344761 (+)	849	WP_021721698.1	DNA-processing protein DprA	Machinery gene
LLA12_RS06450 (LLA12_01365)	topA	1345103..1347154 (+)	2052	WP_080651949.1	type I DNA topoisomerase	-
LLA12_RS06455 (LLA12_01366)	trmFO	1347226..1348569 (+)	1344	WP_017864499.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
LLA12_RS06460 (LLA12_01367)	xerS	1348614..1349684 (-)	1071	WP_003131037.1	tyrosine recombinase XerS	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 32149.20 Da
• Isoelectric Point: 9.5086

>NTDB_id=1144867 LLA12_RS06445 WP_021721698.1 1343913..1344761(+) (dprA) [Lactococcus lactis subsp. lactis strain A12]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQEVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLSKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide

• Length: 849 bp

>NTDB_id=1144867 LLA12_RS06445 WP_021721698.1 1343913..1344761(+) (dprA) [Lactococcus lactis subsp. lactis strain A12]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACAAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGAAGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAGTATCTTTCTAAAAACCA
ACTCATACTATCAGAATATTCAGTTGGAGAAAAGCCACTTCGTTATCATTTTCCTGAACGTAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

• DNA recombination-mediator protein A (DNA_processg_A) (76-270)

3D structure

• AlphaFold DB: S6FQS2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	86.17	100	0.862
dprA	Streptococcus mutans UA159	59.353	98.582	0.585
dprA/cilB/dalA	Streptococcus mitis SK321	54.643	99.291	0.543
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	53.571	99.291	0.532
dprA/cilB/dalA	Streptococcus pneumoniae D39	53.571	99.291	0.532
dprA/cilB/dalA	Streptococcus pneumoniae R6	53.571	99.291	0.532
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	53.571	99.291	0.532
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.571	99.291	0.532
dprA	Legionella pneumophila strain ERS1305867	38.768	97.872	0.379
dprA	Haemophilus influenzae Rd KW20	38.889	95.745	0.372