Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R615_RS13670 Genome accession   NZ_CP006829
Coordinates   3007781..3009478 (+) Length   565 a.a.
NCBI ID   WP_025266213.1    Uniprot ID   -
Organism   Thalassolituus oleivorans R6-15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3002781..3014478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R615_RS13650 (R615_13940) nadC 3004041..3004892 (+) 852 WP_025266209.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R615_RS18680 - 3005172..3005280 (+) 109 Protein_2718 pilin -
  R615_RS18555 (R615_13945) - 3005316..3005660 (+) 345 WP_414625685.1 hypothetical protein -
  R615_RS13665 (R615_13955) - 3005870..3007708 (+) 1839 WP_155990873.1 sulfatase-like hydrolase/transferase -
  R615_RS13670 (R615_13960) pilB 3007781..3009478 (+) 1698 WP_025266213.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R615_RS13675 (R615_13965) pilC 3009523..3010752 (+) 1230 WP_025266214.1 type II secretion system F family protein Machinery gene
  R615_RS13680 (R615_13970) - 3010757..3011644 (+) 888 WP_025266215.1 prepilin peptidase -
  R615_RS13685 (R615_13975) coaE 3011684..3012283 (+) 600 WP_025266216.1 dephospho-CoA kinase -
  R615_RS13690 (R615_13980) yacG 3012320..3012514 (+) 195 WP_015485862.1 DNA gyrase inhibitor YacG -
  R615_RS13695 (R615_13985) - 3012570..3013751 (-) 1182 WP_025266217.1 two-component system response regulator -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61731.86 Da        Isoelectric Point: 5.0219

>NTDB_id=114310 R615_RS13670 WP_025266213.1 3007781..3009478(+) (pilB) [Thalassolituus oleivorans R6-15]
MALLTGLPRRLVEQQLLNQAQAEEILASCKKEKQSFVTLAVAKKLVKARELAVLASEEFGLPLLDLAAFDGELMPKELVS
SELIEKHRVLPLFVRGNRLFVAQSDPSQMIAIDEIKFNTGMTVEAILVEEDKLSVAVDRFLSANDSTLGDLDSALDDIDL
DDPNTRNKEDEQNSNAGGDDAPIVRFVNKILLDAIKSGASDVHFEPYEKRYRVRYRTDGILLEVSSPPVNLAGKIAARLK
VMSQMDISERRLPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEPEQKDLYLSALAKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFAAALRSFLRQDPDVVMVGEIRDLE
TAQIAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPSFNIATSVSLIIAQRLARRLCVQCKVPAEIPEHTLKEVGFTDE
LLADAEIFKPVGCEHCNHGYKGRVGIYEVVRVTDAISRIIMEGGNSIEISDAARKEGFADLRTSALKKVAQGVTSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=114310 R615_RS13670 WP_025266213.1 3007781..3009478(+) (pilB) [Thalassolituus oleivorans R6-15]
ATGGCCTTATTGACCGGCCTTCCTCGTCGTCTCGTTGAGCAACAACTTCTCAACCAAGCGCAAGCCGAAGAGATACTCGC
CAGTTGTAAAAAAGAAAAACAAAGCTTTGTCACACTCGCGGTTGCGAAAAAGTTGGTCAAAGCGAGAGAGCTTGCGGTTT
TGGCCTCTGAAGAATTTGGCTTACCACTGTTAGACCTCGCCGCTTTTGACGGCGAATTGATGCCAAAAGAACTGGTCAGC
TCTGAACTCATCGAAAAGCACCGTGTGCTACCACTATTTGTTCGCGGTAACCGATTATTTGTAGCGCAAAGCGATCCATC
GCAAATGATCGCTATCGATGAAATCAAGTTCAATACCGGCATGACGGTAGAAGCGATACTGGTTGAAGAAGATAAACTCA
GCGTTGCCGTTGATCGTTTTTTATCCGCCAACGACAGTACCTTAGGTGATCTTGATAGCGCGCTCGACGATATCGATTTA
GATGATCCAAACACCCGAAACAAAGAGGACGAACAAAATAGCAACGCAGGAGGCGACGACGCCCCTATCGTTCGTTTTGT
AAATAAAATTTTATTAGACGCAATTAAATCCGGAGCATCCGATGTTCACTTTGAGCCCTACGAAAAGCGTTACCGTGTGC
GTTATCGTACCGATGGTATTTTATTAGAGGTCTCCAGCCCTCCAGTGAATTTGGCTGGTAAAATTGCGGCACGTCTAAAA
GTTATGTCGCAAATGGATATTTCTGAACGTCGTCTTCCTCAAGATGGTCGTATCAAGATGAAACTGTCAAAAACCAAAGC
CATCGACTTCCGCGTCAATACCTTACCCACGTTATGGGGCGAAAAAATCGTACTGCGTATCCTTGATCCGTCTTCAGCTA
AGCTAGGGATTGATGCTTTAGGCTATGAGCCAGAACAAAAAGACTTGTACTTAAGTGCCTTGGCTAAACCACAAGGTATG
ATCTTGGTCACTGGGCCTACCGGTTCCGGCAAGACTGTTTCGCTCTATACTGGCCTGAATATTTTAAACGTGGAAGGTAC
CAATATTTCCACGGCAGAAGATCCCGTTGAAATTAACTTGGAAGGTATCAACCAAGTTAACGTGAATGCTCGTGTCGGTC
TCGACTTTGCTGCCGCGCTGCGATCCTTCTTACGCCAAGACCCAGATGTGGTCATGGTGGGTGAGATTCGCGATTTGGAA
ACTGCTCAAATTGCGATTAAAGCTGCACAAACAGGTCACTTAGTGCTGTCTACTCTACACACCAATAGTGCAGCCGAAAC
ACTGACACGTTTGGCCAATATGGGGGTTCCGTCGTTTAATATCGCAACTTCAGTAAGCCTAATTATTGCCCAGCGTTTAG
CTCGTCGCCTATGCGTGCAGTGTAAAGTGCCCGCGGAAATCCCAGAGCACACATTAAAAGAAGTGGGCTTTACCGATGAA
TTGCTTGCAGACGCCGAAATATTTAAACCGGTAGGTTGTGAACACTGCAACCATGGCTACAAAGGCCGCGTCGGTATATA
TGAAGTCGTTCGCGTTACGGATGCAATATCGCGGATCATCATGGAAGGTGGTAACTCCATTGAAATATCGGATGCTGCTC
GAAAAGAAGGATTTGCCGATTTGCGTACTTCGGCGCTTAAGAAAGTCGCTCAAGGCGTCACTAGCCTAGAAGAAGCAAAC
CGTGTGACAACCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.832

100

0.628
  pilB Acinetobacter baylyi ADP1

62.257

100

0.625
  pilB Legionella pneumophila strain ERS1305867

54.991

100

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

52.12

100

0.522
  pilB Vibrio cholerae strain A1552

50.617

100

0.508
  pilF Neisseria gonorrhoeae MS11

50.709

99.823

0.506
  pilB Vibrio campbellii strain DS40M4

50.177

100

0.503
  pilF Thermus thermophilus HB27

36.106

100

0.361
  pilB/pilB1 Synechocystis sp. PCC 6803

40.637

88.85

0.361

Multiple sequence alignment