Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   DQN52_RS09935 Genome accession   NZ_LS483426
Coordinates   1836361..1837629 (-) Length   422 a.a.
NCBI ID   WP_003787779.1    Uniprot ID   A0AAX2J5Z2
Organism   Kingella kingae strain NCTC10529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831361..1842629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN52_RS09900 (NCTC10529_01929) - 1831567..1832760 (+) 1194 WP_003787791.1 acetate kinase -
  DQN52_RS09905 (NCTC10529_01930) - 1832835..1833455 (-) 621 WP_003787790.1 L-threonylcarbamoyladenylate synthase -
  DQN52_RS09910 (NCTC10529_01931) pncC 1833465..1833941 (-) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  DQN52_RS09915 (NCTC10529_01932) zapD 1833961..1834722 (-) 762 WP_003787787.1 cell division protein ZapD -
  DQN52_RS09925 (NCTC10529_01933) coaE 1834837..1835445 (-) 609 WP_003787784.1 dephospho-CoA kinase -
  DQN52_RS09930 (NCTC10529_01934) pilD 1835447..1836349 (-) 903 WP_003787782.1 A24 family peptidase Machinery gene
  DQN52_RS09935 (NCTC10529_01935) pilG 1836361..1837629 (-) 1269 WP_003787779.1 type II secretion system F family protein Machinery gene
  DQN52_RS09940 (NCTC10529_01936) pilF 1837676..1839361 (-) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DQN52_RS09945 (NCTC10529_01937) lysA 1839547..1840773 (-) 1227 WP_003787776.1 diaminopimelate decarboxylase -
  DQN52_RS10840 (NCTC10529_01938) lptM 1840773..1840934 (-) 162 WP_003787775.1 LPS translocon maturation chaperone LptM -
  DQN52_RS09950 (NCTC10529_01939) cyaY 1841025..1841348 (+) 324 WP_099046165.1 iron donor protein CyaY -
  DQN52_RS09955 (NCTC10529_01940) pgeF 1841498..1842277 (+) 780 WP_003787769.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46018.37 Da        Isoelectric Point: 10.0411

>NTDB_id=1141195 DQN52_RS09935 WP_003787779.1 1836361..1837629(-) (pilG) [Kingella kingae strain NCTC10529]
MAAKKPVKKASVKKAAAAKPVKKEKGNRYQFEGRNLSTDSIVRGEVIAKNEDEARQKLARRQIKVLQLTKMKKAKDKKIT
SADIAVFTRQLSTMMKAGLPLMQAFDIVAKGHANASMTAMLMEIRNDVEQGEALGDSFAKHPKYFDKFYCNLVAAGEAGG
VLEGLLDKLATYKEKTEAIKKKVKSALTYPISIVVVAVALVIVMMMFVLPEFGKVYSSMGAELPPLTQFMMNISNAFVEF
GWLIILALIGGVVALIQFHKRSHAFQKKVDTWLLKTPIFGDIVRKATIARWARTTATLFTAGVPLVEVLDSVAGAAGNII
YEEATREIRSKVNQGISLTSGMQATNLFPNMVVQMASIGEESGALDDMLNKTAEFYEDEVDAAVGMLSSLMEPIIMVVLG
GIIGVILVAMYLPLFNLGNVVA

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=1141195 DQN52_RS09935 WP_003787779.1 1836361..1837629(-) (pilG) [Kingella kingae strain NCTC10529]
ATGGCTGCAAAAAAACCTGTTAAAAAAGCATCTGTAAAAAAAGCAGCGGCTGCCAAGCCTGTTAAGAAAGAAAAAGGCAA
TCGCTACCAGTTTGAAGGTCGCAATTTAAGTACCGATTCAATCGTACGTGGCGAAGTGATTGCTAAAAATGAAGACGAAG
CACGTCAGAAGTTAGCGCGTCGCCAAATTAAGGTGTTGCAACTGACTAAAATGAAAAAGGCAAAAGATAAGAAAATTACT
TCTGCCGACATTGCGGTGTTTACGCGCCAACTTTCTACCATGATGAAAGCAGGTTTACCATTGATGCAAGCGTTTGATAT
TGTGGCAAAAGGTCATGCCAATGCATCTATGACTGCCATGCTGATGGAAATTCGCAACGATGTGGAACAAGGTGAAGCAT
TGGGCGATTCGTTTGCTAAGCACCCTAAATATTTTGACAAATTCTATTGCAACTTGGTTGCCGCAGGTGAGGCAGGCGGT
GTATTGGAAGGCTTGTTAGACAAACTGGCAACCTATAAAGAAAAAACCGAAGCGATTAAGAAAAAAGTGAAATCGGCTTT
GACTTACCCAATTTCTATTGTGGTGGTGGCGGTTGCCTTGGTGATTGTGATGATGATGTTCGTGTTGCCAGAGTTTGGTA
AGGTGTACAGTAGCATGGGCGCAGAATTGCCACCGTTGACGCAATTTATGATGAATATTTCCAATGCGTTTGTGGAATTT
GGTTGGTTGATTATTTTGGCTTTGATTGGCGGTGTGGTTGCGCTGATTCAATTCCATAAGAGATCTCATGCGTTCCAGAA
AAAAGTGGATACATGGTTGTTAAAAACGCCAATTTTTGGTGATATTGTTCGTAAAGCAACGATTGCACGTTGGGCACGTA
CTACTGCAACCTTATTTACTGCGGGTGTGCCATTGGTGGAAGTATTGGATTCGGTAGCTGGTGCAGCAGGTAATATTATT
TACGAAGAAGCCACTCGCGAAATTCGTTCCAAAGTAAACCAAGGTATTTCTTTGACATCGGGTATGCAGGCAACTAATTT
ATTCCCGAACATGGTAGTGCAAATGGCTTCCATTGGTGAAGAATCTGGCGCGTTGGATGATATGTTAAACAAAACCGCTG
AATTCTATGAAGACGAAGTTGATGCTGCGGTGGGAATGTTGTCATCTTTGATGGAACCCATCATTATGGTGGTATTAGGC
GGTATCATTGGTGTGATTCTGGTCGCAATGTACTTGCCATTGTTTAACTTGGGTAATGTAGTGGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68.08

95.024

0.647
  pilG Neisseria meningitidis 44/76-A

67.332

95.024

0.64
  pilC Legionella pneumophila strain ERS1305867

47.619

94.55

0.45
  pilC Acinetobacter baumannii D1279779

45.455

91.232

0.415
  pilC Acinetobacter baylyi ADP1

45.078

91.469

0.412
  pilC Pseudomonas stutzeri DSM 10701

49.565

81.754

0.405
  pilC Vibrio cholerae strain A1552

37.981

98.578

0.374

Multiple sequence alignment