Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DQM57_RS00215 Genome accession   NZ_LS483369
Coordinates   37727..39403 (+) Length   558 a.a.
NCBI ID   WP_108043478.1    Uniprot ID   -
Organism   Neisseria cinerea strain NCTC10294     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 32727..44403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM57_RS00175 (NCTC10294_00034) rpmB 33061..33294 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  DQM57_RS00180 (NCTC10294_00035) rpmG 33323..33478 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  DQM57_RS00185 (NCTC10294_00036) ubiM 33692..34876 (-) 1185 WP_039854249.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  DQM57_RS00190 (NCTC10294_00037) rpmA 35121..35393 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  DQM57_RS00195 (NCTC10294_00038) rplU 35421..35729 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  DQM57_RS00200 (NCTC10294_00039) - 35952..36926 (+) 975 WP_108043479.1 polyprenyl synthetase family protein -
  DQM57_RS00205 (NCTC10294_00040) - 36931..37377 (+) 447 WP_003676595.1 DUF441 domain-containing protein -
  DQM57_RS00215 (NCTC10294_00042) pilF 37727..39403 (+) 1677 WP_108043478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DQM57_RS00220 (NCTC10294_00043) - 39496..39705 (-) 210 WP_039854241.1 DNA gyrase inhibitor YacG -
  DQM57_RS00225 (NCTC10294_00044) coaE 39698..40330 (-) 633 WP_111726190.1 dephospho-CoA kinase -
  DQM57_RS00230 (NCTC10294_00045) pilD 40332..41192 (-) 861 WP_111726191.1 A24 family peptidase Machinery gene
  DQM57_RS00235 (NCTC10294_00046) pilG 41266..42498 (-) 1233 WP_107996268.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62150.51 Da        Isoelectric Point: 6.0470

>NTDB_id=1138931 DQM57_RS00215 WP_108043478.1 37727..39403(+) (pilF) [Neisseria cinerea strain NCTC10294]
MSVGLLRTLVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGIISPKSLAALIARVFSYSVLDLSYYPRHRVLMGVLTEEQ
MVEFRCVPIFRRDNKVFFAVSDPTQMPQIQKTVFASGISVELVIVEDDRLAGLLEWVGSRSTALLQEISAEQEEQESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPVAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLDAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNEKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPSCKQEVVRPPVSALKEAGFTDEDLAKDWKLY
RAVGCDRCRGKGYKGRAGVYEVMPISEEMQRVIMNHGTEVDIMDVAYKEGMVDLRRSGLLKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1138931 DQM57_RS00215 WP_108043478.1 37727..39403(+) (pilF) [Neisseria cinerea strain NCTC10294]
ATGAGTGTTGGTCTTTTAAGGACTTTGGTACAAAACCAAGTGGTCACTGTCGAACAGGCAGAGCATTACTATAAGGAGTC
GCAGGCAGGGAAGGAAGTTTTGCCGATGTTGTTTTCAGACGGCATTATTTCCCCAAAGTCGCTTGCTGCATTGATTGCCA
GGGTGTTCAGTTATTCGGTTCTTGATTTGAGTTACTATCCGCGCCATAGGGTACTGATGGGCGTGCTGACCGAGGAACAG
ATGGTAGAGTTCCGTTGTGTGCCGATTTTCCGACGGGATAACAAGGTATTTTTTGCGGTTTCTGATCCGACGCAGATGCC
TCAGATTCAGAAGACCGTTTTTGCGTCAGGGATTTCGGTTGAGTTGGTGATTGTCGAAGACGATCGGCTGGCCGGGCTGT
TGGAATGGGTGGGTTCGCGCTCGACTGCGTTGCTTCAGGAAATCAGTGCGGAACAGGAGGAGCAGGAAAGCCATACTCTG
TATATTGATAATGAAGAGGCGGAAGACGGTCCTGTTCCAAGGTTTATCCATAAAACTTTGTCGGATGCTTTACGCAGTGG
GGCATCGGATATTCATTTTGAGTTTTACGAACATAATGCGCGTATCCGGTTCCGTGTGGACGGGCAGCTTCGCGAGGTAG
TCCAACCCCCGGTTGCGGTGAGGGGGCAGCTTGCTTCTCGGATTAAGGTGATGTCGCGCTTGGATATTTCCGAAAAACGC
ATACCTCAGGACGGTAGGATGCAATTGACCTTCCAAAAGGGTGGCAAGCCTGTCGATTTCCGTGTCAGTACGTTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAATTCTGATGCTGCTTCTTTGAATATCGATCAGCTTGGTTTCGAGC
CGTTTCAGAAGAAATTGCTGTTGGATGCTATCCACCGTCCTTACGGCATGGTGTTGGTAACCGGTCCGACAGGTTCGGGA
AAGACTGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAACATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGTATTAATCAGGTTAATGTTAATGAAAAGCAGGGGTTAACTTTTGCCGCAGCCTTGAAGTCTTTTT
TGCGTCAGGATCCGGACATTATCATGGTTGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCTACCCTGCATACTAATAATGCGCCGGCAACATTGTCACGTATGCTGAATATGGGTGTTGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGCCTGTTGAGGAGACTGTGCCCGAGTTGTAAACAGG
AAGTGGTACGTCCGCCTGTTTCTGCGTTGAAGGAGGCGGGTTTTACAGATGAAGACCTTGCAAAGGATTGGAAGCTCTAC
CGTGCGGTTGGTTGCGACCGCTGTCGGGGCAAGGGTTATAAGGGGCGTGCCGGTGTCTATGAGGTTATGCCTATCAGCGA
GGAAATGCAGCGTGTCATTATGAACCATGGTACAGAAGTGGATATTATGGACGTTGCTTATAAGGAAGGTATGGTCGATT
TGCGCCGTTCCGGTTTGTTGAAAGTTATGCAGGGTATTACTTCGTTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-138)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

93.548

100

0.935
  pilB Acinetobacter baumannii D1279779

49.561

100

0.505
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

48.161

100

0.493
  pilB Vibrio cholerae strain A1552

46.986

100

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.457
  pilB Vibrio campbellii strain DS40M4

44.149

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.505

95.878

0.369
  pilF Thermus thermophilus HB27

37.132

97.491

0.362

Multiple sequence alignment