Detailed information NTG1137575

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQL21_RS01615
• Genome accession: NZ_LS483343
• Coordinates: 296398..296853 (+)
• Length: 151 a.a.
• NCBI ID: WP_040804182.1
• Uniprot ID: A0A2X3XY68
• Organism: Streptococcus ferus strain NCTC12278
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 291398..301853

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL21_RS01580 (NCTC12278_00302)	-	291488..292180 (+)	693	WP_018031090.1	response regulator transcription factor	-
DQL21_RS01585 (NCTC12278_00303)	-	292143..293492 (+)	1350	WP_018031091.1	cell wall metabolism sensor histidine kinase WalK	-
DQL21_RS01590 (NCTC12278_00304)	comYA	293545..294486 (+)	942	WP_018031092.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL21_RS01595 (NCTC12278_00305)	comYB	294383..295456 (+)	1074	WP_269458426.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL21_RS01600 (NCTC12278_00306)	comYC	295453..295767 (+)	315	WP_018031094.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL21_RS01605 (NCTC12278_00307)	comYD	295754..296167 (+)	414	WP_018031095.1	competence type IV pilus minor pilin ComGD	Machinery gene
DQL21_RS01610 (NCTC12278_00308)	comGE	296157..296432 (+)	276	WP_018031096.1	competence type IV pilus minor pilin ComGE	-
DQL21_RS01615 (NCTC12278_00309)	comYF	296398..296853 (+)	456	WP_040804182.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL21_RS01620 (NCTC12278_00310)	comGG	296831..297226 (+)	396	WP_018031098.1	competence type IV pilus minor pilin ComGG	-
DQL21_RS01625 (NCTC12278_00311)	comYH	297276..298232 (+)	957	WP_018031099.1	class I SAM-dependent methyltransferase	Machinery gene
DQL21_RS01630 (NCTC12278_00312)	-	298290..299483 (+)	1194	WP_018031100.1	acetate kinase	-
DQL21_RS01635 (NCTC12278_00313)	-	299619..299816 (+)	198	WP_018031101.1	helix-turn-helix transcriptional regulator	-
DQL21_RS01640 (NCTC12278_00314)	-	299877..300326 (+)	450	WP_018031102.1	hypothetical protein	-
DQL21_RS01645 (NCTC12278_00315)	-	300336..301169 (+)	834	WP_018031103.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 151 a.a.
• Molecular weight: 17238.74 Da
• Isoelectric Point: 9.0968

>NTDB_id=1137575 DQL21_RS01615 WP_040804182.1 296398..296853(+) (comYF) [Streptococcus ferus strain NCTC12278]
MGRLFLNLFKSKLRAFTLLECLIALLVLAGSLSIYQGLTKVLSANIHYVANSQEENWLLFCQQFRAELERSRLKSVSPGR
LTVTRDGQELAFGKSKSDDFRKTNADGRGFQPMLWEVASASFSQEDDDIRLELRFTNGLERSFIYAFTKQT

Nucleotide

• Length: 456 bp

>NTDB_id=1137575 DQL21_RS01615 WP_040804182.1 296398..296853(+) (comYF) [Streptococcus ferus strain NCTC12278]
ATGGGGAGATTGTTTTTGAACTTATTCAAGAGTAAATTACGCGCTTTTACACTTTTGGAATGTCTGATTGCTCTCTTGGT
CCTAGCAGGCTCCTTGTCCATTTATCAGGGGCTAACCAAGGTTCTATCGGCTAATATTCACTATGTCGCCAACAGCCAAG
AGGAAAACTGGCTCCTGTTTTGTCAGCAATTCCGTGCAGAGTTGGAAAGAAGCCGGCTCAAGTCGGTCTCTCCCGGCAGG
CTTACGGTGACGCGTGATGGACAAGAGCTGGCGTTTGGCAAGTCAAAGTCAGATGATTTTAGAAAAACGAATGCCGACGG
CCGTGGCTTTCAGCCCATGCTTTGGGAAGTTGCTTCAGCTTCTTTCAGTCAAGAGGACGACGACATTCGATTGGAACTTA
GGTTTACAAATGGTTTGGAAAGGAGCTTTATTTATGCTTTTACGAAGCAGACTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (44-140)

3D structure

• AlphaFold DB: A0A2X3XY68

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	61.429	92.715	0.57
comYF	Streptococcus mutans UA159	60.714	92.715	0.563
comGF/cglF	Streptococcus mitis NCTC 12261	47.826	91.391	0.437
comGF/cglF	Streptococcus pneumoniae Rx1	44.604	92.053	0.411
comGF/cglF	Streptococcus pneumoniae D39	44.604	92.053	0.411
comGF/cglF	Streptococcus pneumoniae R6	44.604	92.053	0.411
comGF/cglF	Streptococcus pneumoniae TIGR4	44.604	92.053	0.411
comGF/cglF	Streptococcus mitis SK321	44.203	91.391	0.404
comGF	Lactococcus lactis subsp. cremoris KW2	42.857	92.715	0.397