Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLVWC_RS05200 Genome accession   NZ_LR962896
Coordinates   1254780..1256504 (+) Length   574 a.a.
NCBI ID   WP_053045683.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 554     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1249780..1261504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLVWC_RS05175 (X12_001033) - 1250705..1250860 (-) 156 Protein_1009 SymE family type I addiction module toxin -
  ACLVWC_RS05180 (X12_001034) coaE 1250960..1251583 (-) 624 WP_411861412.1 dephospho-CoA kinase -
  ACLVWC_RS05185 (X12_001035) - 1251597..1252460 (-) 864 WP_002812278.1 prepilin peptidase -
  ACLVWC_RS05190 (X12_001036) pilC 1252467..1253726 (-) 1260 WP_039525808.1 type II secretion system F family protein Machinery gene
  ACLVWC_RS05195 - 1254071..1254478 (+) 408 WP_039529754.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACLVWC_RS05200 (X12_001038) pilB 1254780..1256504 (+) 1725 WP_053045683.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLVWC_RS05205 (X12_001039) pilR 1256802..1258196 (-) 1395 WP_053045681.1 sigma-54-dependent transcriptional regulator Regulator
  ACLVWC_RS05210 (X12_001040) - 1258389..1260002 (-) 1614 WP_016901263.1 sensor histidine kinase -
  ACLVWC_RS05215 (X12_001041) sucC 1260234..1261403 (+) 1170 WP_053045679.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62394.48 Da        Isoelectric Point: 5.6006

>NTDB_id=1134929 ACLVWC_RS05200 WP_053045683.1 1254780..1256504(+) (pilB) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 554]
MNQNLVGITGIARRLVQDGVIDESVARSAMEQASTAKIPLPQWFSDKKLVSAPQLAAANAVEFGMPLMDVSVFDASQNAV
KLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGDDDE
GMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLN
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLE
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHAL
LAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVKAAH
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1134929 ACLVWC_RS05200 WP_053045683.1 1254780..1256504(+) (pilB) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 554]
ATGAATCAGAATTTGGTTGGCATTACCGGTATCGCGCGGCGTCTAGTTCAAGATGGCGTTATAGACGAGTCTGTGGCCCG
CTCGGCAATGGAGCAGGCGTCAACAGCAAAGATTCCTTTGCCGCAGTGGTTTTCCGATAAGAAGCTGGTCTCAGCGCCTC
AACTTGCCGCTGCCAATGCAGTCGAGTTCGGTATGCCGCTGATGGACGTTTCCGTGTTTGACGCGAGCCAGAACGCCGTC
AAGCTGGTCAGTGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGT
GAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTTCATACCAACCTGGTAGTCGAGCCGATCCTGGTCGACG
AGGACCAGATCCGTCGCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTAGGCTCGTCGCTCGGTGACGACGATGAG
GGGATGGGCGACCTGGACGTGTCGGCCGGTGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGA
CGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCACTTCGAGC
CCTATGAAGACGACTATCGGGTGCGCTTGCGCATCGATGGGCTGCTGAAGAACGTGGCCAAGGCACCGGTGAAGCTGAAC
CAGCGCATCGCGGCACGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGCCGCATCAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAAAAGGTGGTGTTGC
GTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTGTTCCTGGAG
GCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACTGCACT
GGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGGTTGCCCGGCGTCAATCAAG
TGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACATCATCATG
GTCGGCGAAATCCGCGACTTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCATATGGTGCTGTCCACGCT
GCATACCAACGACGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATTGCCCCTTACAACATCACTTCGTCGGTGA
CCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAGCATGCGTTG
CTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGTATCGAGTTGTATGAGGCGGTCGGTTGCGACGAGTGCACCGA
AGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGCA
ATGCGATGCAGATTGCCGAGGCGGCGCAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTCAAGGCCGCGCAC
GGGGTGACCAGCCTGGCCGAGATCAACCGGGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.81

98.955

0.552
  pilB Acinetobacter baumannii D1279779

55.634

98.955

0.551
  pilB Legionella pneumophila strain ERS1305867

52.197

99.129

0.517
  pilB Vibrio cholerae strain A1552

48.793

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.768

98.955

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.167

100

0.462
  pilB Vibrio campbellii strain DS40M4

46.046

99.129

0.456
  pilF Thermus thermophilus HB27

40.192

90.592

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

91.463

0.361

Multiple sequence alignment