Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACLVWB_RS15720 Genome accession   NZ_LR962645
Coordinates   3663925..3664347 (-) Length   140 a.a.
NCBI ID   WP_026064717.1    Uniprot ID   -
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1567     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3655335..3682296 3663925..3664347 within 0


Gene organization within MGE regions


Location: 3655335..3682296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLVWB_RS15685 (X12_003126) - 3655335..3655718 (+) 384 WP_289986106.1 hypothetical protein -
  ACLVWB_RS15690 (X12_003127) sucD 3655795..3656670 (-) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  ACLVWB_RS15695 (X12_003128) sucC 3656695..3657864 (-) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -
  ACLVWB_RS15700 (X12_003129) - 3658096..3659709 (+) 1614 WP_016901263.1 sensor histidine kinase -
  ACLVWB_RS15705 (X12_003130) pilR 3659902..3661296 (+) 1395 WP_016901261.1 sigma-54-dependent transcriptional regulator Regulator
  ACLVWB_RS15710 (X12_003131) pilB 3661510..3663237 (-) 1728 WP_289882848.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLVWB_RS15715 (X12_003132) - 3663384..3663845 (-) 462 WP_345783685.1 pilin -
  ACLVWB_RS15720 (X12_003133) pilA 3663925..3664347 (-) 423 WP_026064717.1 pilin Machinery gene
  ACLVWB_RS15725 (X12_003134) pilC 3664781..3665938 (+) 1158 WP_386051418.1 type II secretion system F family protein Machinery gene
  ACLVWB_RS15730 (X12_003135) - 3665945..3666808 (+) 864 WP_002812278.1 prepilin peptidase -
  ACLVWB_RS15735 (X12_003136) coaE 3666822..3667445 (+) 624 WP_016904087.1 dephospho-CoA kinase -
  ACLVWB_RS15740 (X12_003137) - 3667971..3668369 (+) 399 WP_016904088.1 SymE family type I addiction module toxin -
  ACLVWB_RS15745 (X12_003138) - 3668474..3669808 (-) 1335 WP_016904089.1 sensor histidine kinase -
  ACLVWB_RS15750 (X12_003139) - 3669801..3670478 (-) 678 WP_006448355.1 response regulator transcription factor -
  ACLVWB_RS15755 (X12_003140) - 3670510..3670980 (-) 471 WP_026064719.1 hypothetical protein -
  ACLVWB_RS15760 (X12_003141) rimK 3671207..3672112 (-) 906 WP_166767257.1 30S ribosomal protein S6--L-glutamate ligase -
  ACLVWB_RS15765 (X12_003142) glgX 3672602..3674734 (+) 2133 WP_016904092.1 glycogen debranching protein GlgX -
  ACLVWB_RS15770 (X12_003143) - 3675406..3675798 (-) 393 WP_023904915.1 H-NS family nucleoid-associated regulatory protein -
  ACLVWB_RS15775 (X12_003144) - 3675887..3676273 (-) 387 WP_026064907.1 hypothetical protein -
  ACLVWB_RS15780 (X12_003145) - 3676848..3677360 (-) 513 WP_049805061.1 ImmA/IrrE family metallo-endopeptidase -
  ACLVWB_RS15785 (X12_003146) - 3677345..3677551 (-) 207 WP_374057769.1 ImmA/IrrE family metallo-endopeptidase -
  ACLVWB_RS15790 (X12_003147) - 3678095..3678664 (+) 570 WP_087962438.1 hypothetical protein -
  ACLVWB_RS15795 (X12_003148) - 3678736..3680016 (+) 1281 WP_080591431.1 retropepsin-like aspartic protease -
  ACLVWB_RS15800 (X12_003149) - 3680506..3680868 (+) 363 WP_016904888.1 hypothetical protein -
  ACLVWB_RS15805 (X12_003150) - 3680965..3682296 (+) 1332 WP_016905121.1 IS4 family transposase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 13903.03 Da        Isoelectric Point: 8.1053

>NTDB_id=1134869 ACLVWB_RS15720 WP_026064717.1 3663925..3664347(-) (pilA) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1567]
MNKQQGFTLIELMIVVAIIAILAAIALPAYQDYTIRARVSEAAVLASGAKTTVAENIANNGGTIAAGACLGVDTTPPATT
NVASVTCGANTGAIAVVTTAKAGAVTLTFTPQPAAGIAGTKWTCVATGGKAAYYPAECRS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1134869 ACLVWB_RS15720 WP_026064717.1 3663925..3664347(-) (pilA) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1567]
ATGAACAAGCAACAAGGCTTCACACTTATTGAATTAATGATTGTTGTGGCGATCATTGCCATTCTGGCGGCGATTGCTTT
GCCTGCATATCAAGACTATACAATTCGCGCACGCGTCTCTGAGGCTGCTGTGCTTGCTTCGGGTGCTAAAACTACAGTTG
CTGAGAACATTGCAAACAACGGCGGAACAATAGCTGCCGGCGCATGCCTTGGGGTTGATACTACTCCGCCAGCAACCACG
AATGTCGCTAGCGTTACCTGTGGCGCCAATACCGGTGCAATTGCAGTTGTCACGACCGCTAAGGCCGGTGCAGTTACCTT
GACCTTCACTCCGCAGCCTGCTGCCGGCATTGCTGGTACTAAGTGGACCTGTGTTGCGACTGGCGGTAAGGCAGCCTATT
ATCCGGCTGAATGCCGTAGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

46.429

100

0.557
  comP Acinetobacter baylyi ADP1

52.349

100

0.557
  pilA2 Legionella pneumophila str. Paris

54.676

99.286

0.543
  pilA2 Legionella pneumophila strain ERS1305867

53.957

99.286

0.536
  pilA/pilA1 Eikenella corrodens VA1

40.789

100

0.443
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

99.286

0.407
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.486

100

0.386

Multiple sequence alignment