Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7A86_RS15510 Genome accession   NZ_LR861803
Coordinates   3706892..3708616 (-) Length   574 a.a.
NCBI ID   WP_119131075.1    Uniprot ID   A0A8E4E577
Organism   Xanthomonas euroxanthea isolate     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3701892..3713616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7A86_RS15495 (XSP_003115) sucC 3702017..3703186 (-) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -
  H7A86_RS15500 (XSP_003116) - 3703418..3705031 (+) 1614 WP_104544483.1 HAMP domain-containing sensor histidine kinase -
  H7A86_RS15505 (XSP_003117) pilR 3705213..3706607 (+) 1395 WP_164739181.1 sigma-54 dependent transcriptional regulator Regulator
  H7A86_RS15510 (XSP_003118) pilB 3706892..3708616 (-) 1725 WP_119131075.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7A86_RS21145 (XSP_003119) - 3708930..3709337 (-) 408 WP_119131074.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H7A86_RS15520 (XSP_003120) pilC 3709683..3710942 (+) 1260 WP_119131073.1 type II secretion system F family protein Machinery gene
  H7A86_RS15525 (XSP_003121) - 3710949..3711812 (+) 864 WP_119131072.1 A24 family peptidase -
  H7A86_RS15530 (XSP_003122) coaE 3711826..3712431 (+) 606 WP_119131071.1 dephospho-CoA kinase -
  H7A86_RS15535 (XSP_003123) - 3712641..3712969 (+) 329 Protein_3065 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62498.54 Da        Isoelectric Point: 5.4486

>NTDB_id=1132442 H7A86_RS15510 WP_119131075.1 3706892..3708616(-) (pilB) [Xanthomonas euroxanthea isolate]
MNQNLVGITGIARRLVQDGVIDESVARSAMEQASTAKIPLPQWFSDKKLVSAPQLAAANAVEFGMPLMDVSVFDASQNAV
KLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGDDDD
DMGDLDVSAGDEDMGAGGDAGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLS
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLE
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRRSSLPEHAL
LAEGFTAEQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALVKAAH
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1132442 H7A86_RS15510 WP_119131075.1 3706892..3708616(-) (pilB) [Xanthomonas euroxanthea isolate]
ATGAATCAGAATTTGGTTGGCATTACCGGTATCGCGCGGCGTCTAGTTCAAGATGGCGTCATAGATGAGTCTGTTGCTCG
CTCGGCAATGGAGCAGGCGTCAACGGCAAAGATTCCTTTGCCACAGTGGTTTTCCGATAAAAAGCTAGTCTCAGCGCCTC
AACTTGCTGCTGCCAACGCGGTCGAGTTCGGTATGCCGCTGATGGATGTGTCTGTGTTCGATGCCAGCCAGAACGCAGTC
AAGTTGGTCAGTGAAGAGTTGCTCCAAAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGATTGTTCGTGGGGGT
GAGCAACCCGACTCAGACCAGGGCGCTGGACGATATCAAGTTCCATACGAACCTGGTGGTCGAGCCGATTCTGGTCGATG
AGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGTCGCTTGGCTCATCGCTCGGTGACGACGATGAC
GACATGGGGGATTTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATGCCGGGGTCGATGCCAAGGGCGA
CGACACGCCGGTGGTGAAATTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCATTTCGAGC
CCTATGAAGACGACTATCGGGTGCGCTTGCGCATCGACGGCCTGCTGAAGAATGTGGCCAAGGCGCCGGTGAAGCTGAGC
CAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAAAAGCGGGTGCCGCAGGACGGGCGCATCAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCTTGTTCGGCGAGAAGGTGGTGCTGC
GTATTCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTGTTCCTGGAG
GCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACGGCGCT
GGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAGGACCCGGTCGAAATCCGCCTGCCTGGCGTCAACCAGG
TGCAGCAGAACAACAAGCGCGGCATGACGTTTGCGGCGGCGTTGCGCTCGTTCCTGCGCCAGGACCCTGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCTGTCCACGCT
GCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGTATTGCGCCGTACAACATCACCTCGTCGGTGA
CGTTGGTGATTGCGCAGCGTCTGGCGCGACGGTTGTGCAACAACTGCAAGCGCAGATCGAGCCTGCCCGAGCATGCATTG
CTGGCGGAGGGGTTCACTGCAGAGCAGCTCGCTGCTGGTATCGAGCTGTACGAGGCAGTCGGTTGCGACGAGTGCACCGA
AGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATTGGCGCGATCGTGCTGGAAGGCGGCA
ACGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGCATCCGCGACCTGCGCCAGTCTGCGCTGGTCAAGGCCGCGCAC
GGGGTGACCAGCCTGGCCGAGATCAATCGCGTAACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.955

0.552
  pilB Acinetobacter baylyi ADP1

55.458

98.955

0.549
  pilB Legionella pneumophila strain ERS1305867

52.548

99.129

0.521
  pilF Neisseria gonorrhoeae MS11

49.648

98.955

0.491
  pilB Vibrio cholerae strain A1552

48.174

100

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

45.502

100

0.458
  pilB Vibrio campbellii strain DS40M4

45.87

99.129

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.548

92.509

0.366
  pilF Thermus thermophilus HB27

39.808

90.592

0.361

Multiple sequence alignment