Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   H1W81_RS02060 Genome accession   NZ_LR824002
Coordinates   379966..381066 (+) Length   366 a.a.
NCBI ID   WP_375708571.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_67     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 374966..386066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W81_RS02050 (STHERMO_0422) - 378700..379062 (+) 363 WP_011226630.1 DUF1033 family protein -
  H1W81_RS02055 (STHERMO_0423) comGA/cglA/cilD 379143..380084 (+) 942 WP_180484814.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W81_RS02060 (STHERMO_0424) comYB 379966..381066 (+) 1101 WP_375708571.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1W81_RS02065 (STHERMO_0425) comYC 381063..381389 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1W81_RS02070 (STHERMO_0426) comYD 381349..381777 (+) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  H1W81_RS02075 (STHERMO_0427) comGE 381749..382042 (+) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  H1W81_RS02080 (STHERMO_0428) comYF 382026..382463 (+) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1W81_RS02085 (STHERMO_0429) comGG 382441..382758 (+) 318 WP_011226623.1 competence type IV pilus minor pilin ComGG -
  H1W81_RS02090 (STHERMO_0430) comYH 382803..383759 (+) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  H1W81_RS02095 (STHERMO_0431) - 383815..385008 (+) 1194 WP_022096898.1 acetate kinase -
  H1W81_RS02100 (STHERMO_0432) - 385257..385454 (+) 198 WP_011681682.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41772.92 Da        Isoelectric Point: 10.0449

>NTDB_id=1132317 H1W81_RS02060 WP_375708571.1 379966..381066(+) (comYB) [Streptococcus thermophilus isolate STH_CIRM_67]
MPEKISKTIRLPTGISSWKVWLNKDVSLRGMSKGKKLKISQQVKVIQLFKQLLKAGFTLTEIVAFLERSHLLKEASLSLM
KESLIRGDRLDQMLALVGFSDNIVTQISLADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKNNWAVQLVQIFPQLFFVSLCGLLVLGLILYLWVKRQSALVFYRRMAKIPFIGQTVRLYTTAYYAREWG
NLLGQGVDLLDLVALMQEQKSKLFRELGADLEEALMLGQSFPERIASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QNFFNRLNKATTFVQPLIFVIVAVVIVMIYVAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=1132317 H1W81_RS02060 WP_375708571.1 379966..381066(+) (comYB) [Streptococcus thermophilus isolate STH_CIRM_67]
TTGCCAGAGAAAATTTCCAAAACCATTCGTCTACCAACTGGAATCAGCAGTTGGAAGGTTTGGTTAAACAAGGATGTCTC
ACTGAGAGGGATGTCCAAGGGGAAAAAATTAAAGATTAGTCAGCAAGTTAAGGTTATCCAGCTCTTCAAACAACTTTTAA
AGGCCGGTTTTACCTTAACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGCTGAAAGAAGCATCCTTGTCTCTTATG
AAAGAGAGTTTAATACGCGGTGATAGGTTGGATCAGATGTTAGCGTTAGTGGGGTTTTCGGACAATATTGTTACTCAGAT
TTCTCTTGCTGACAAGCACGGTAATCTTCTAGGGAGTCTAACAAAGATTGAAACCTATATGCTTCGTATGACAAAGGTTC
GCAAGAAACTCATGGAGGTGGCGACTTATCCCATACTACTTCTGGGTTTTCTGATTCTGATTATGCTAGGACTTAAAAAT
TATCTTCTACCCCAACTCTTAGAGGGTGATGGTAAGAATAATTGGGCTGTACAGTTGGTTCAAATTTTTCCCCAGCTTTT
TTTTGTGAGTTTGTGTGGACTCCTTGTGTTGGGTTTAATTCTCTATCTATGGGTGAAACGACAGTCAGCCCTTGTTTTTT
ATAGGCGAATGGCCAAAATCCCTTTTATTGGTCAGACAGTAAGGCTTTACACGACCGCCTATTATGCTAGGGAATGGGGA
AATTTATTAGGTCAAGGCGTTGATTTGCTAGATTTGGTGGCTCTTATGCAAGAGCAAAAGTCTAAACTCTTCCGTGAGCT
GGGAGCCGATTTGGAAGAAGCCCTGATGCTGGGACAGAGTTTTCCAGAACGTATTGCCAGTCATCCGTTTTTTACTAAGG
AGCTCTCACTAATTATTGCTTATGGGGAGGCCAATGCGAGGTTGGGATATGAGTTGGAAGTTTATGCCGAGGAGGTTTGG
CAAAACTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTGTTGCAGTTGTGATTGT
AATGATCTATGTAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.678

93.443

0.511
  comYB Streptococcus mutans UA159

54.678

93.443

0.511
  comYB Streptococcus gordonii str. Challis substr. CH1

51.17

93.443

0.478
  comGB/cglB Streptococcus mitis NCTC 12261

49.853

92.623

0.462
  comGB/cglB Streptococcus mitis SK321

49.112

92.35

0.454
  comGB/cglB Streptococcus pneumoniae Rx1

48.817

92.35

0.451
  comGB/cglB Streptococcus pneumoniae D39

48.817

92.35

0.451
  comGB/cglB Streptococcus pneumoniae R6

48.817

92.35

0.451
  comGB/cglB Streptococcus pneumoniae TIGR4

48.817

92.35

0.451
  comGB Lactococcus lactis subsp. cremoris KW2

45.758

90.164

0.413

Multiple sequence alignment