Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NSCAC_RS00495 Genome accession   NZ_LR778175
Coordinates   90471..92180 (-) Length   569 a.a.
NCBI ID   WP_269474122.1    Uniprot ID   A0A7G1Q7V6
Organism   Candidatus Nitrosacidococcus tergens isolate NSCAC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 85471..97180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSCAC_RS00460 (NSCAC_0095) - 86074..86319 (+) 246 WP_197744500.1 zinc-finger domain-containing protein -
  NSCAC_RS00465 (NSCAC_0096) - 86330..86530 (-) 201 WP_197745206.1 DUF2905 domain-containing protein -
  NSCAC_RS00470 (NSCAC_0097) - 86564..87526 (+) 963 WP_197744501.1 Nudix family hydrolase -
  NSCAC_RS00475 (NSCAC_0098) yacG 87529..87735 (-) 207 WP_197744502.1 DNA gyrase inhibitor YacG -
  NSCAC_RS00480 (NSCAC_0099) coaE 87742..88395 (-) 654 WP_197744503.1 dephospho-CoA kinase -
  NSCAC_RS00485 (NSCAC_0100) pilD 88352..89221 (-) 870 WP_197744504.1 A24 family peptidase Machinery gene
  NSCAC_RS00490 (NSCAC_0101) pilC 89249..90463 (-) 1215 WP_197744505.1 type II secretion system F family protein Machinery gene
  NSCAC_RS00495 (NSCAC_0102) pilB 90471..92180 (-) 1710 WP_269474122.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NSCAC_RS00500 lptM 92300..92434 (+) 135 WP_197744506.1 LPS translocon maturation chaperone LptM -
  NSCAC_RS00505 (NSCAC_0103) lysA 92443..93693 (+) 1251 WP_197744507.1 diaminopimelate decarboxylase -
  NSCAC_RS00510 (NSCAC_0104) - 93715..94383 (+) 669 WP_197744508.1 O-methyltransferase -
  NSCAC_RS00515 (NSCAC_0105) dapF 94390..95232 (+) 843 WP_197744509.1 diaminopimelate epimerase -
  NSCAC_RS00520 (NSCAC_0106) xerC 95402..96319 (+) 918 WP_197744510.1 tyrosine recombinase XerC -
  NSCAC_RS00525 (NSCAC_0107) - 96518..96847 (+) 330 WP_197744511.1 c-type cytochrome -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63380.51 Da        Isoelectric Point: 6.0436

>NTDB_id=1130235 NSCAC_RS00495 WP_269474122.1 90471..92180(-) (pilB) [Candidatus Nitrosacidococcus tergens isolate NSCAC1]
MTPSKAQIATLSHCLAKHNLLSESDALLYYKKSLQENISFISYLTQHKLVDSLSIAQVASQEFGMPLFNLNTLDISYSPQ
NLIEEKLVYEHRIFPLFKRGNKLFIAIADPTNQQQALSNIKFYTGVSTEPILVEEDKLSSMIEKINQDHRKVLAELSNTL
ETVASTQNEDEVEENFIESDKEDAPIVRFVNRLLLDAINRGASDIHFEPYEKTYRVRYRQDGILHEVATPPVNLSSRITS
RIKVMARLDIAERRVPQDGRMKAKISDKKSIDFRVSTCPTLFGEKAVLRILDPTSAQIGIHSLGYELEQQKIFLDTIHQS
YGMVLVTGPTGSGKTVSLYTALNILNTGNINISTAEDPAEINVPGINQVNVHPKVGLTFASALRSFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLVNMGVPSYNIASAVSLIIAQRLVRRLCTHCKTEEHIPDQVLLEEGF
TEEQIADRPIFYKANGCDYCAGGYKGRIGIYQVMPVSEIMGEIIMCGGNSMELADQAKKEGISDLRQSGVKKIISGVTSL
EEVNRVTKE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1130235 NSCAC_RS00495 WP_269474122.1 90471..92180(-) (pilB) [Candidatus Nitrosacidococcus tergens isolate NSCAC1]
ATGACACCATCGAAAGCACAAATTGCCACCTTATCCCATTGTCTTGCAAAACATAATTTATTAAGCGAATCAGATGCTCT
ACTTTATTATAAAAAATCTCTACAAGAAAATATCTCTTTTATCTCTTACCTAACTCAGCATAAGCTAGTCGATAGTTTGA
GTATTGCTCAAGTTGCTTCTCAAGAATTTGGGATGCCTCTATTCAACTTAAATACTTTAGATATTAGCTATAGCCCTCAA
AATCTCATTGAAGAAAAATTAGTCTACGAACATCGAATATTTCCTTTATTCAAGCGGGGAAATAAGCTATTTATTGCAAT
TGCTGATCCTACTAACCAACAACAGGCACTCAGTAATATTAAGTTTTATACCGGAGTAAGCACTGAACCTATCTTAGTAG
AGGAAGATAAACTCTCTTCCATGATTGAAAAAATCAATCAGGATCATAGAAAAGTGCTGGCAGAACTAAGCAATACCTTG
GAAACTGTTGCAAGTACTCAAAATGAAGATGAAGTAGAAGAAAATTTTATTGAGTCTGATAAGGAAGATGCTCCTATTGT
TCGCTTTGTAAATCGTCTGCTTCTTGATGCTATCAATCGTGGAGCTTCAGATATTCATTTTGAGCCTTATGAAAAAACCT
ATCGGGTGCGTTATCGACAAGATGGTATTCTACACGAAGTGGCTACTCCTCCCGTAAATTTATCCTCTCGAATTACTTCT
CGAATTAAAGTAATGGCACGACTGGATATTGCAGAGCGTCGGGTTCCCCAAGATGGGCGGATGAAAGCAAAAATTTCTGA
TAAAAAATCCATTGATTTTCGAGTAAGTACTTGTCCTACCTTATTTGGAGAAAAAGCAGTACTTAGAATTTTAGATCCTA
CCAGTGCCCAAATTGGTATTCACTCTTTAGGCTATGAATTAGAGCAGCAAAAGATATTTTTAGATACTATCCATCAGTCT
TATGGCATGGTATTGGTCACTGGACCAACTGGAAGTGGTAAAACCGTTTCTCTTTACACCGCACTTAATATTCTAAATAC
AGGGAATATAAATATTTCTACCGCTGAAGATCCTGCAGAAATTAACGTACCAGGGATTAATCAAGTTAATGTGCATCCCA
AAGTAGGACTTACTTTTGCCAGCGCTCTTAGATCTTTTCTACGTCAAGATCCTGATGTGATTATGGTGGGTGAGATTCGA
GATTTAGAAACTGCAGAGATTGCTATTAAAGCGGCACAAACAGGGCATATGGTACTCTCTACCCTACATACTAACGATGC
TCCTCAAACCCTAACTAGATTAGTCAATATGGGAGTGCCTTCTTATAACATCGCTTCTGCTGTTTCTTTAATTATTGCAC
AACGGTTAGTCCGTCGTTTATGCACTCATTGTAAAACAGAGGAGCATATCCCTGATCAGGTACTACTAGAAGAAGGATTT
ACTGAAGAGCAGATCGCAGATAGACCTATTTTCTATAAAGCAAATGGTTGTGATTATTGTGCTGGAGGATATAAAGGTCG
TATTGGTATATACCAAGTAATGCCTGTCTCGGAAATTATGGGAGAAATAATCATGTGTGGAGGAAATTCAATGGAATTAG
CAGATCAGGCAAAAAAGGAGGGTATTTCAGACTTACGTCAATCTGGGGTTAAAAAAATTATTTCTGGGGTAACTAGCTTA
GAGGAAGTAAATCGAGTAACTAAAGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G1Q7V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.626

98.77

0.54
  pilB Acinetobacter baumannii D1279779

55.41

94.2

0.522
  pilB Acinetobacter baylyi ADP1

54.428

95.255

0.518
  pilF Neisseria gonorrhoeae MS11

50.352

99.824

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

47.88

99.473

0.476
  pilB Vibrio campbellii strain DS40M4

46.69

100

0.471
  pilB Vibrio cholerae strain A1552

49.71

90.861

0.452
  pilF Thermus thermophilus HB27

39.729

90.685

0.36
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.837

86.116

0.36

Multiple sequence alignment