Detailed information NTG1130233

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NSCAC_RS00485
• Genome accession: NZ_LR778175
• Coordinates: 88352..89221 (-)
• Length: 289 a.a.
• NCBI ID: WP_197744504.1
• Uniprot ID: A0A7G1Q7X9
• Organism: Candidatus Nitrosacidococcus tergens isolate NSCAC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 83352..94221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NSCAC_RS00455 (NSCAC_0094)	nadA	84959..86053 (+)	1095	WP_197745204.1	quinolinate synthase NadA	-
NSCAC_RS00460 (NSCAC_0095)	-	86074..86319 (+)	246	WP_197744500.1	zinc-finger domain-containing protein	-
NSCAC_RS00465 (NSCAC_0096)	-	86330..86530 (-)	201	WP_197745206.1	DUF2905 domain-containing protein	-
NSCAC_RS00470 (NSCAC_0097)	-	86564..87526 (+)	963	WP_197744501.1	Nudix family hydrolase	-
NSCAC_RS00475 (NSCAC_0098)	yacG	87529..87735 (-)	207	WP_197744502.1	DNA gyrase inhibitor YacG	-
NSCAC_RS00480 (NSCAC_0099)	coaE	87742..88395 (-)	654	WP_197744503.1	dephospho-CoA kinase	-
NSCAC_RS00485 (NSCAC_0100)	pilD	88352..89221 (-)	870	WP_197744504.1	A24 family peptidase	Machinery gene
NSCAC_RS00490 (NSCAC_0101)	pilC	89249..90463 (-)	1215	WP_197744505.1	type II secretion system F family protein	Machinery gene
NSCAC_RS00495 (NSCAC_0102)	pilB	90471..92180 (-)	1710	WP_269474122.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NSCAC_RS00500	lptM	92300..92434 (+)	135	WP_197744506.1	LPS translocon maturation chaperone LptM	-
NSCAC_RS00505 (NSCAC_0103)	lysA	92443..93693 (+)	1251	WP_197744507.1	diaminopimelate decarboxylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 33079.17 Da
• Isoelectric Point: 6.8935

>NTDB_id=1130233 NSCAC_RS00485 WP_197744504.1 88352..89221(-) (pilD) [Candidatus Nitrosacidococcus tergens isolate NSCAC1]
MEFISFFDDYPSLFLISSFILGLIIGSFLNVIIYRLPLMIERTWRSQCAELQGNPLPKSESFNLWLPHSHCPHCKKIVYP
WENIPILSYLILRGRCSHCKAHISPRYPLVELLTGMFSLVVALHFGITWEGLAALTLTYALIVLSFIDFDHQILPDDITL
PFLWLGLILSLFDLFTDAYSSIIGTVAGYLFFWLIYQLFRILARKEGMGYGDFKLLSMLGAWLGWKMLPIIILFSSFGGA
IFGSLWLYITKQTKETPLSFGPYIAVSGWIALMWGDDINHFYLSLSKFG

Nucleotide

• Length: 870 bp

>NTDB_id=1130233 NSCAC_RS00485 WP_197744504.1 88352..89221(-) (pilD) [Candidatus Nitrosacidococcus tergens isolate NSCAC1]
ATGGAATTTATATCTTTCTTTGATGATTATCCAAGCTTGTTTTTAATTTCATCATTCATATTAGGGCTAATTATAGGTAG
TTTCTTAAATGTAATTATTTATCGCTTACCTTTGATGATAGAGAGAACCTGGAGATCTCAATGTGCAGAACTACAGGGCA
ACCCATTGCCTAAATCTGAATCATTTAATTTATGGCTCCCCCACTCCCATTGCCCTCATTGTAAAAAAATTGTTTACCCT
TGGGAGAATATCCCTATATTGAGCTATCTCATACTTCGAGGGCGTTGTAGCCACTGTAAAGCTCATATTTCCCCTCGTTA
CCCTCTAGTAGAGCTCCTTACAGGAATGTTTTCGCTCGTGGTTGCACTTCATTTTGGTATCACTTGGGAAGGGTTAGCCG
CACTTACATTAACTTATGCACTGATTGTACTAAGTTTTATTGATTTTGATCATCAAATCTTGCCAGATGATATTACTCTA
CCCTTTCTTTGGTTAGGACTCATTCTAAGTCTATTTGATCTATTTACCGATGCCTACAGTAGTATTATAGGAACTGTGGC
TGGATATCTTTTCTTTTGGCTTATTTATCAGTTATTCCGCATTCTAGCCCGTAAAGAAGGTATGGGATATGGGGATTTTA
AGCTCTTGAGTATGTTAGGAGCATGGCTTGGATGGAAGATGCTTCCGATTATTATTTTGTTCTCTTCTTTTGGAGGAGCT
ATATTCGGAAGTTTATGGCTTTATATTACTAAGCAAACAAAAGAAACCCCTCTCTCTTTTGGTCCTTATATTGCTGTTTC
AGGATGGATAGCCTTAATGTGGGGGGATGATATTAATCATTTTTATTTATCTCTCTCTAAGTTTGGTTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: A0A7G1Q7X9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.352	98.27	0.495
pilD	Vibrio campbellii strain DS40M4	47.535	98.27	0.467
pilD	Acinetobacter baumannii D1279779	46.667	98.616	0.46
pilD	Acinetobacter nosocomialis M2	46.667	98.616	0.46
pilD	Neisseria gonorrhoeae MS11	47.126	90.311	0.426