Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   AACL56_RS01860 Genome accession   NZ_LR743507
Coordinates   390237..390782 (-) Length   181 a.a.
NCBI ID   WP_339088137.1    Uniprot ID   -
Organism   Variovorax paradoxus isolate vvax     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 385237..395782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACL56_RS01850 - 388760..388924 (-) 165 WP_339092954.1 hypothetical protein -
  AACL56_RS01855 - 388910..389801 (+) 892 Protein_369 integrase core domain-containing protein -
  AACL56_RS01860 (VVAX_00373) pilE 390237..390782 (-) 546 WP_339088137.1 pilin Machinery gene
  AACL56_RS01865 (VVAX_00374) - 390990..391793 (-) 804 WP_339088138.1 3',5'-cyclic-nucleotide phosphodiesterase -
  AACL56_RS01870 (VVAX_00375) - 391823..394075 (-) 2253 WP_339088139.1 CHASE2 domain-containing protein -
  AACL56_RS01875 (VVAX_00376) - 394124..394792 (-) 669 WP_339088140.1 FHA domain-containing protein -
  AACL56_RS01880 (VVAX_00377) - 394859..395668 (-) 810 WP_339088141.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 18642.19 Da        Isoelectric Point: 8.4603

>NTDB_id=1129786 AACL56_RS01860 WP_339088137.1 390237..390782(-) (pilE) [Variovorax paradoxus isolate vvax]
MNRRTLARRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEAVINATSAKSLVSEAFQTDGLAGVKAAADTFN
GKDAGEKSTKYIESIAISKDDGSEGQITVTLKNNDSAGFPAEVKNTTLIFSPNVQKAALADKAGAIDWACGSITATTAGT
RGLGSAATGTLPAKYAPAECR

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1129786 AACL56_RS01860 WP_339088137.1 390237..390782(-) (pilE) [Variovorax paradoxus isolate vvax]
ATGAACCGTCGTACTCTCGCCCGCCGCGCACAGGCCGGCTTTACCCTTATCGAACTGATGATCGTTGTGGCGATCATCGG
TATCTTGGCTGCGGTGGCTCTGCCAGCGTATCAAGACTACACGGTCCGCGCAAAGGTCTCGGAAGCTGTGATCAATGCGA
CGTCCGCAAAGTCGCTCGTCAGCGAAGCTTTCCAAACGGACGGACTGGCAGGCGTGAAGGCTGCAGCGGATACGTTCAAT
GGTAAGGACGCTGGCGAAAAGTCGACCAAGTACATCGAGAGCATTGCCATCAGCAAGGATGACGGCTCCGAAGGCCAAAT
CACGGTCACGCTGAAGAACAACGACAGTGCTGGCTTCCCCGCCGAAGTGAAGAACACGACGCTCATTTTCTCGCCGAATG
TGCAGAAGGCTGCTTTGGCTGACAAGGCTGGCGCTATCGACTGGGCTTGCGGCAGCATTACGGCAACGACCGCCGGCACC
CGCGGCCTGGGTTCCGCCGCTACGGGCACGCTGCCCGCCAAGTACGCCCCTGCTGAATGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

51.613

100

0.53
  pilA Ralstonia pseudosolanacearum GMI1000

41.53

100

0.42
  pilA2 Legionella pneumophila strain ERS1305867

41.279

95.028

0.392
  pilA2 Legionella pneumophila str. Paris

40.698

95.028

0.387
  comP Acinetobacter baylyi ADP1

38.636

97.238

0.376
  pilE Neisseria gonorrhoeae strain FA1090

37.64

98.343

0.37
  pilE Neisseria gonorrhoeae MS11

36.872

98.895

0.365

Multiple sequence alignment