Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   GPW69_RS00770 Genome accession   NZ_LR738724
Coordinates   116851..117285 (+) Length   144 a.a.
NCBI ID   WP_044762030.1    Uniprot ID   -
Organism   Streptococcus suis isolate 9401240     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 111851..122285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW69_RS00735 - 112591..112965 (+) 375 WP_024385120.1 DUF1033 family protein -
  GPW69_RS00740 - 112993..113943 (-) 951 WP_074391326.1 S66 peptidase family protein -
  GPW69_RS00745 comYA 114029..114979 (+) 951 WP_074391327.1 competence type IV pilus ATPase ComGA Machinery gene
  GPW69_RS00750 comYB 114891..115928 (+) 1038 WP_269472402.1 competence type IV pilus assembly protein ComGB Machinery gene
  GPW69_RS00755 comYC 115930..116211 (+) 282 WP_074391328.1 competence type IV pilus major pilin ComGC Machinery gene
  GPW69_RS00760 comGD 116192..116599 (+) 408 WP_074391329.1 competence type IV pilus minor pilin ComGD -
  GPW69_RS00765 comYE 116571..116864 (+) 294 WP_024401507.1 competence type IV pilus minor pilin ComGE Machinery gene
  GPW69_RS00770 comGF/cglF 116851..117285 (+) 435 WP_044762030.1 competence type IV pilus minor pilin ComGF Machinery gene
  GPW69_RS00775 comGG 117263..117676 (+) 414 WP_074391330.1 competence type IV pilus minor pilin ComGG -
  GPW69_RS00780 comYH 117726..118679 (+) 954 WP_024414316.1 class I SAM-dependent methyltransferase Machinery gene
  GPW69_RS00785 - 118729..119916 (+) 1188 WP_044680792.1 acetate kinase -
  GPW69_RS00790 - 120233..120784 (+) 552 WP_074391332.1 folate family ECF transporter S component -
  GPW69_RS00795 - 120840..122096 (+) 1257 WP_074391333.1 bifunctional folylpolyglutamate synthase/dihydrofolate synthase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16334.63 Da        Isoelectric Point: 6.9822

>NTDB_id=1129584 GPW69_RS00770 WP_044762030.1 116851..117285(+) (comGF/cglF) [Streptococcus suis isolate 9401240]
MLKSKIPAFTLLECLVALVILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQADLVKVENGKVYVDKGG
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1129584 GPW69_RS00770 WP_044762030.1 116851..117285(+) (comGF/cglF) [Streptococcus suis isolate 9401240]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCCTTAGTCATTCTTTCAGGTAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGATCAGGCTGACTTGGTCAAGGTTGAAAATGGCAAGGTCTATGTTGATAAGGGTGGT
CAGGCTTTGGCCTTTGGTAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGCTACCAGCCCATGCT
CTATCAGGTGGACAGCGCAGCTATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGTTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

53.901

97.917

0.528
  comGF/cglF Streptococcus pneumoniae Rx1

53.901

97.917

0.528
  comGF/cglF Streptococcus pneumoniae D39

53.901

97.917

0.528
  comGF/cglF Streptococcus pneumoniae R6

53.901

97.917

0.528
  comGF/cglF Streptococcus mitis NCTC 12261

54.478

93.056

0.507
  comYF Streptococcus mutans UA140

50

100

0.5
  comYF Streptococcus mutans UA159

49.306

100

0.493
  comGF/cglF Streptococcus mitis SK321

51.493

93.056

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

46.809

97.917

0.458

Multiple sequence alignment