Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5P1_RS14605 Genome accession   NZ_LR594689
Coordinates   2983360..2983875 (+) Length   171 a.a.
NCBI ID   WP_068679367.1    Uniprot ID   A0A2J7W4B0
Organism   Variovorax sp. WDL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2978360..2988875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P1_RS14585 (G3W93_RS14600) - 2978490..2979299 (+) 810 WP_068679358.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  E5P1_RS14590 (G3W93_RS14605) - 2979368..2980018 (+) 651 WP_068679360.1 FHA domain-containing protein -
  E5P1_RS14595 (G3W93_RS14610) - 2980058..2982334 (+) 2277 WP_068679362.1 adenylate/guanylate cyclase domain-containing protein -
  E5P1_RS14600 (G3W93_RS14615) - 2982348..2983157 (+) 810 WP_068679365.1 3',5'-cyclic-nucleotide phosphodiesterase -
  E5P1_RS14605 (G3W93_RS14620) pilA 2983360..2983875 (+) 516 WP_068679367.1 pilin Machinery gene
  E5P1_RS14610 (G3W93_RS14625) - 2983985..2985157 (+) 1173 WP_068679369.1 glycosyltransferase family 2 protein -
  E5P1_RS14615 (G3W93_RS14630) - 2985168..2985944 (+) 777 WP_068679371.1 hypothetical protein -
  E5P1_RS14620 (G3W93_RS14635) - 2985941..2987119 (+) 1179 WP_146039304.1 hypothetical protein -
  E5P1_RS14625 (G3W93_RS14640) - 2987146..2988255 (+) 1110 WP_083944481.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  E5P1_RS14630 (G3W93_RS14645) - 2988252..2988842 (+) 591 WP_068679377.1 acyltransferase -

Sequence

Protein


Download         Length: 171 a.a.        Molecular weight: 17202.56 Da        Isoelectric Point: 9.1201

>NTDB_id=1128268 E5P1_RS14605 WP_068679367.1 2983360..2983875(+) (pilA) [Variovorax sp. WDL1]
MNRRSIARNVQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVTDGLSLAGSAKTAVAENAANGAPFASGWTSPAAT
RNVASIGITEGTGVITITYQNEIDGGATLTLTPLDGSRASGAALAGSSTASTIPSAGSITWVCASAGSTVANSNVVKGTI
KDKFVPAECRT

Nucleotide


Download         Length: 516 bp        

>NTDB_id=1128268 E5P1_RS14605 WP_068679367.1 2983360..2983875(+) (pilA) [Variovorax sp. WDL1]
ATGAACCGTCGTTCCATCGCACGCAACGTGCAAAAGGGTTTCACCCTTATCGAACTGATGATCGTTGTGGCGATCATTGG
CATCTTGGCGGCTGTGGCGCTGCCGGCGTATCAGGACTACACCGTCCGCGCAAAGGTTACCGACGGCCTTTCCTTGGCTG
GAAGCGCGAAGACTGCTGTGGCTGAAAACGCGGCGAACGGCGCCCCATTTGCTTCTGGCTGGACGTCTCCCGCCGCTACC
CGCAATGTCGCCAGCATCGGCATTACCGAAGGCACCGGCGTAATCACCATTACCTACCAGAATGAGATAGATGGTGGCGC
GACGCTGACCCTCACCCCGCTCGACGGTTCGCGCGCTTCTGGCGCAGCGCTTGCAGGCTCATCGACCGCTTCGACCATCC
CTTCTGCCGGCTCTATCACATGGGTTTGCGCTTCCGCCGGGAGCACCGTTGCAAACTCAAACGTTGTGAAGGGCACCATC
AAGGACAAGTTCGTCCCGGCTGAGTGCCGCACGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J7W4B0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.847

100

0.526
  pilA2 Legionella pneumophila str. Paris

49.697

96.491

0.48
  pilA2 Legionella pneumophila strain ERS1305867

49.091

96.491

0.474
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.5

100

0.45
  comP Acinetobacter baylyi ADP1

40.351

100

0.404
  pilE Neisseria gonorrhoeae MS11

37.853

100

0.392
  pilE Neisseria gonorrhoeae strain FA1090

37.5

98.246

0.368

Multiple sequence alignment