Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3W89_RS33520 Genome accession   NZ_LR594659
Coordinates   5720466..5720969 (+) Length   167 a.a.
NCBI ID   WP_162577021.1    Uniprot ID   A0A6P2FK62
Organism   Variovorax sp. PBL-H6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5715466..5725969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3W89_RS26935 (H6CHR_05382) - 5715595..5716404 (+) 810 WP_162577017.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  G3W89_RS26940 (H6CHR_05383) - 5716473..5717123 (+) 651 WP_162577018.1 FHA domain-containing protein -
  G3W89_RS26945 (H6CHR_05384) - 5717163..5719433 (+) 2271 WP_162577019.1 adenylate/guanylate cyclase domain-containing protein -
  G3W89_RS26950 (H6CHR_05385) - 5719454..5720263 (+) 810 WP_162577020.1 3',5'-cyclic-nucleotide phosphodiesterase -
  G3W89_RS33520 (H6CHR_05386) pilA 5720466..5720969 (+) 504 WP_162577021.1 pilin Machinery gene
  G3W89_RS26960 (H6CHR_05387) - 5722060..5725953 (-) 3894 WP_162577022.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 17045.43 Da        Isoelectric Point: 9.4057

>NTDB_id=1128178 G3W89_RS33520 WP_162577021.1 5720466..5720969(+) (pilA) [Variovorax sp. PBL-H6]
MNRRSIARNVQKGFTLIELMIVVAIIGILAAVALPAYQDYVKRAKVTEGLSLASSAKTTIAENAANGKKYDAGWTAPSAT
AAVKSVAIDNTTGAVTITFDKPVEDGKTLILRPFTGPNTTPVALPDSSSGTYDAPTGNIAWACGAAGAGAPATAGTLDAK
YAPSSCR

Nucleotide


Download         Length: 504 bp        

>NTDB_id=1128178 G3W89_RS33520 WP_162577021.1 5720466..5720969(+) (pilA) [Variovorax sp. PBL-H6]
ATGAACCGTCGTTCCATTGCACGCAACGTGCAAAAGGGTTTCACCCTTATCGAGTTGATGATCGTTGTGGCGATCATTGG
TATTCTCGCTGCAGTCGCGCTGCCTGCCTACCAAGACTATGTGAAGCGTGCCAAGGTCACCGAGGGACTGTCGCTCGCCT
CGTCTGCCAAGACCACCATCGCCGAGAACGCCGCAAACGGCAAGAAGTACGATGCGGGCTGGACTGCTCCGTCTGCTACT
GCTGCTGTGAAGTCGGTCGCGATCGACAACACCACGGGCGCCGTGACGATCACGTTCGACAAGCCCGTCGAAGATGGCAA
GACCCTGATCCTGCGCCCCTTCACCGGCCCGAACACAACGCCCGTCGCGCTGCCTGACTCCAGCTCTGGCACCTACGACG
CGCCTACCGGCAATATCGCCTGGGCTTGCGGAGCCGCTGGTGCTGGTGCCCCTGCAACCGCTGGCACGCTGGACGCCAAG
TACGCACCATCTAGCTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-167)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2FK62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

47.727

100

0.503
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.384

100

0.455
  pilA2 Legionella pneumophila strain ERS1305867

45.679

97.006

0.443
  pilA2 Legionella pneumophila str. Paris

45.062

97.006

0.437
  comP Acinetobacter baylyi ADP1

39.655

100

0.413
  pilA/pilA1 Eikenella corrodens VA1

34.783

100

0.383
  pilE Neisseria gonorrhoeae MS11

38.182

98.802

0.377

Multiple sequence alignment