Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAty_RS13530 Genome accession   NZ_CP005986
Coordinates   2754276..2755982 (+) Length   568 a.a.
NCBI ID   WP_004869544.1    Uniprot ID   -
Organism   Acidithiobacillus caldus ATCC 51756     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2749276..2760982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAty_RS13500 (Acaty_c2704) - 2749285..2749890 (+) 606 WP_004869526.1 glutathione S-transferase family protein -
  ACAty_RS13505 (Acaty_c2705) xth 2749895..2750671 (+) 777 WP_004869529.1 exodeoxyribonuclease III -
  ACAty_RS13510 (Acaty_c2706) hemH 2750668..2751648 (+) 981 WP_004869533.1 ferrochelatase -
  ACAty_RS13515 (Acaty_c2707) - 2751750..2752235 (+) 486 WP_004869537.1 peptidylprolyl isomerase -
  ACAty_RS13520 (Acaty_c2708) - 2752232..2753425 (+) 1194 WP_226047575.1 anhydro-N-acetylmuramic acid kinase -
  ACAty_RS13525 (Acaty_c2709) aroE 2753428..2754300 (+) 873 WP_004869542.1 shikimate dehydrogenase -
  ACAty_RS13530 (Acaty_c2710) pilB 2754276..2755982 (+) 1707 WP_004869544.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAty_RS13535 (Acaty_c2711) - 2756027..2757580 (+) 1554 WP_004869546.1 PAS domain-containing sensor histidine kinase -
  ACAty_RS13540 (Acaty_c2712) pilR 2757577..2758944 (+) 1368 WP_004869549.1 sigma-54 dependent transcriptional regulator Regulator
  ACAty_RS13545 (Acaty_c2713) - 2758934..2759719 (+) 786 WP_004869552.1 A24 family peptidase -
  ACAty_RS13550 (Acaty_c2714) coaE 2759738..2760325 (+) 588 WP_004869555.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62501.09 Da        Isoelectric Point: 5.9136

>NTDB_id=112752 ACAty_RS13530 WP_004869544.1 2754276..2755982(+) (pilB) [Acidithiobacillus caldus ATCC 51756]
MALSPDTLTLNPMLRALAAQGLGDEATLRELANDPKRGNRPLLFHLVDKGAVKASALMAYLSNRYNVPMLDLEAVQIDDT
VVAKVDRQLMARHQVLPLSRHRDTLFLAVADPTDFKAIEEIKFNTGLQVTPILVEADKLSKAVAAVTSSMQGKLEDLFVD
DGKKSGESDEFDLGRDAEGVDDAPVVRFVQQLLLDAIQKGVSDIHLEPYERDIRVRYRLDGVLQDVLHPPIALREGVTSR
LKILCKLDISERRLPQDGRLRVRVPPARIIDFRVSFLPTNFGETIVLRLLDPASSKVPIEALGFLPEQRKAFEDAIHRPY
GMILVTGPTGSGKTTTLYTALNILNTGDVNISTAEDPVEIPVYGINQVNINERIGLNFAAALRSFLRQDPDIIMVGEVRD
LETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGAVHLVMAQRLARKLCPVCKTPLKIPEQALLEAGFA
VDDLQGWRPLGPKGCDECNQTGYKGRIGLYQVMPVSDAMRDIIMRGGTSMDLARQAQTEGVLSMRQAGLQRVREGLTSLE
EVLRVTNL

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=112752 ACAty_RS13530 WP_004869544.1 2754276..2755982(+) (pilB) [Acidithiobacillus caldus ATCC 51756]
ATGGCTCTCAGCCCAGACACCCTGACCCTCAACCCCATGCTGCGGGCCCTGGCAGCCCAGGGCTTGGGCGATGAGGCCAC
CCTGCGCGAGCTGGCCAATGACCCCAAGCGTGGCAACCGCCCCCTGCTTTTTCATCTGGTGGATAAAGGGGCCGTAAAGG
CGTCGGCCCTCATGGCCTACCTGTCCAATCGCTACAACGTCCCCATGCTGGATCTGGAAGCGGTGCAGATAGACGACACG
GTAGTGGCCAAGGTGGATCGGCAACTCATGGCGCGCCATCAGGTCCTACCCCTGTCCCGCCATCGCGACACCCTTTTCCT
TGCCGTGGCCGATCCCACGGACTTCAAGGCCATCGAAGAGATCAAGTTCAACACTGGCCTGCAGGTCACCCCCATCCTGG
TGGAGGCGGACAAACTCTCCAAGGCCGTCGCCGCCGTCACCAGCAGCATGCAGGGCAAGCTCGAGGATCTCTTCGTCGAC
GATGGCAAGAAATCCGGAGAGTCGGACGAGTTCGACCTGGGTAGGGATGCCGAGGGCGTCGACGACGCCCCGGTCGTGCG
CTTCGTCCAGCAGCTGCTGCTGGACGCCATCCAGAAAGGCGTGTCCGATATCCACCTCGAACCCTACGAGCGCGACATTC
GCGTGCGCTATCGCCTGGACGGAGTATTGCAGGATGTGCTGCACCCGCCCATTGCCCTGCGCGAAGGGGTAACCTCCCGC
CTCAAGATCCTCTGCAAGCTGGACATCTCCGAGCGGCGTCTGCCCCAAGATGGGCGCTTGCGGGTGCGGGTGCCACCGGC
ACGCATCATCGATTTCCGCGTGTCTTTCCTGCCCACCAACTTCGGCGAGACCATCGTCCTGCGCCTGCTGGATCCCGCCA
GTTCCAAGGTGCCCATCGAGGCCCTGGGTTTCCTGCCCGAACAGCGCAAAGCCTTCGAGGACGCCATCCACCGCCCCTAC
GGCATGATCCTCGTCACTGGGCCCACGGGCTCGGGCAAAACCACGACCCTGTATACCGCCCTGAACATCCTCAATACCGG
CGATGTCAATATCAGTACGGCGGAAGACCCCGTGGAAATACCGGTCTACGGCATCAATCAAGTCAACATCAACGAACGCA
TCGGCCTAAACTTCGCCGCCGCCCTGCGTTCCTTCCTCCGGCAAGATCCGGACATCATCATGGTCGGCGAGGTGCGCGAC
CTGGAAACGGCGGAGACGGCCATCAAGGCAGCGCAGACCGGCCACCTGGTCCTCGCCACCCTGCACACCAACGATGCCCC
GCAAAGCCTCACCCGTCTGGAAAACATGGGCATCCCCACCTATAACATAGCCGGCGCCGTGCACCTGGTGATGGCGCAGC
GCCTCGCCCGCAAGCTGTGCCCGGTGTGCAAGACACCCCTGAAGATACCCGAACAGGCCCTGCTGGAGGCTGGTTTTGCC
GTCGACGATCTGCAGGGATGGCGCCCCTTGGGTCCCAAAGGCTGCGACGAGTGCAACCAGACGGGCTACAAGGGGCGAAT
CGGTCTGTATCAGGTGATGCCCGTCAGCGATGCGATGCGCGATATCATCATGCGCGGTGGCACCTCCATGGACCTCGCAC
GCCAGGCGCAGACGGAGGGTGTCCTGAGCATGCGCCAGGCCGGCCTGCAGCGGGTGCGCGAGGGCTTGACGAGTCTCGAA
GAGGTCCTGCGCGTCACCAACCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.04

100

0.495
  pilB Acinetobacter baumannii D1279779

47.78

99.12

0.474
  pilB Acinetobacter baylyi ADP1

45.87

100

0.46
  pilF Neisseria gonorrhoeae MS11

46.981

93.31

0.438
  pilB Vibrio parahaemolyticus RIMD 2210633

46.139

93.486

0.431
  pilB Vibrio campbellii strain DS40M4

45.472

93.31

0.424
  pilB Vibrio cholerae strain A1552

45.283

93.31

0.423
  pilB/pilB1 Synechocystis sp. PCC 6803

36.688

100

0.398
  pilF Thermus thermophilus HB27

38.434

98.944

0.38

Multiple sequence alignment