Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSYRMG_RS18085 Genome accession   NZ_CP005970
Coordinates   4127572..4129677 (+) Length   701 a.a.
NCBI ID   WP_003432146.1    Uniprot ID   A0A193SWW1
Organism   Pseudomonas syringae UMAF0158     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4122572..4134677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRMG_RS18065 (PSYRMG_18400) pilM 4124741..4125805 (+) 1065 WP_003365992.1 pilus assembly protein PilM Machinery gene
  PSYRMG_RS18070 (PSYRMG_18405) - 4125805..4126380 (+) 576 WP_003365994.1 PilN domain-containing protein -
  PSYRMG_RS18075 (PSYRMG_18410) pilO 4126377..4127000 (+) 624 WP_003432147.1 type 4a pilus biogenesis protein PilO -
  PSYRMG_RS18080 (PSYRMG_18415) pilP 4126997..4127524 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  PSYRMG_RS18085 (PSYRMG_18420) pilQ 4127572..4129677 (+) 2106 WP_003432146.1 type IV pilus secretin PilQ Machinery gene
  PSYRMG_RS18090 (PSYRMG_18425) aroK 4129682..4130200 (+) 519 WP_003365999.1 shikimate kinase AroK -
  PSYRMG_RS18095 (PSYRMG_18430) aroB 4130316..4131419 (+) 1104 WP_044308720.1 3-dehydroquinate synthase -
  PSYRMG_RS18100 (PSYRMG_18435) - 4131430..4133058 (+) 1629 WP_003432141.1 AAA family ATPase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75331.66 Da        Isoelectric Point: 5.0294

>NTDB_id=112698 PSYRMG_RS18085 WP_003432146.1 4127572..4129677(+) (pilQ) [Pseudomonas syringae UMAF0158]
MTRILSIIGVSLCIAVLAPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTGSSTTSNVPFVDLGAADATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=112698 PSYRMG_RS18085 WP_003432146.1 4127572..4129677(+) (pilQ) [Pseudomonas syringae UMAF0158]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGGTGCTGGCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCGGCACCGCGCG
GTTACACCACGTCGCAACCAGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGTTGGCGACCAAAAGTCGTGAC
CTGGGCACCGGTAATGCGCGCAGTGTGGTTGTGGTACAGGCTCAGGACCGCACCCGGGTGATCATCAACCTGACCACGCT
TTCGCCGTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCTTCCC
AACCGACCACCGCAATGGGTGCGCAACGGGTCGCGGTGCCACCGCCCACGCCTGCATCCGCCAAACCGTTCATCCCCGCA
GGCGTCAAGGCCATCCGCAATGTCGACTTCCAGCGTGGCGAGTTGGGTGAGGGCAATGTGGTTATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGC
GCGTCCGGCTGGACGTCAAGGACTTCGCCACGCCGGTACAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
TTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGCGTGCGCCCGTTGAC
CAACGAGGATCTGGACCGCCGCGCCGCCGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTTAACTTCCAGGACATTG
ATGTGCGTTCAGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCTGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGACGAGCGCGGTTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGAGCGCCTCGACGAGCTGCGTCGTATCGTGTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTCGGGGTTCGCTGGGGCGGTTCTACCAACACCAGCGGCAGCGGC
AAATGGACAACCTACGGGCTGGATAATAACGGTGACGAGGCCGGTAACACCGGCAGCAGTACTACCTCCAACGTGCCGTT
CGTGGACCTGGGCGCCGCTGATGCGACGACCGGTATCGGCCTGGGCTTCGTGACCAATAACACGCTGCTGGACCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCCTCCCT
GTCGCTTGAGGTAACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTTACCAAGGACGAGCCGGACT
ACCTGAATGCGGTGCTGGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193SWW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.194

100

0.752
  comQ Acinetobacter baylyi ADP1

42.241

99.287

0.419
  pilQ Acinetobacter baumannii D1279779

39.456

100

0.414
  pilQ Acinetobacter baumannii strain A118

39.456

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.551

98.431

0.379
  pilQ Legionella pneumophila strain Lp02

38.551

98.431

0.379

Multiple sequence alignment