Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PSYRMG_RS18065 Genome accession   NZ_CP005970
Coordinates   4124741..4125805 (+) Length   354 a.a.
NCBI ID   WP_003365992.1    Uniprot ID   A0A193SY06
Organism   Pseudomonas syringae UMAF0158     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4119741..4130805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRMG_RS18060 (PSYRMG_18395) - 4122090..4124534 (-) 2445 WP_003365990.1 penicillin-binding protein 1A -
  PSYRMG_RS18065 (PSYRMG_18400) pilM 4124741..4125805 (+) 1065 WP_003365992.1 pilus assembly protein PilM Machinery gene
  PSYRMG_RS18070 (PSYRMG_18405) - 4125805..4126380 (+) 576 WP_003365994.1 PilN domain-containing protein -
  PSYRMG_RS18075 (PSYRMG_18410) pilO 4126377..4127000 (+) 624 WP_003432147.1 type 4a pilus biogenesis protein PilO -
  PSYRMG_RS18080 (PSYRMG_18415) pilP 4126997..4127524 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  PSYRMG_RS18085 (PSYRMG_18420) pilQ 4127572..4129677 (+) 2106 WP_003432146.1 type IV pilus secretin PilQ Machinery gene
  PSYRMG_RS18090 (PSYRMG_18425) aroK 4129682..4130200 (+) 519 WP_003365999.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38104.65 Da        Isoelectric Point: 4.7049

>NTDB_id=112697 PSYRMG_RS18065 WP_003365992.1 4124741..4125805(+) (pilM) [Pseudomonas syringae UMAF0158]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=112697 PSYRMG_RS18065 WP_003365992.1 4124741..4125805(+) (pilM) [Pseudomonas syringae UMAF0158]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCCTTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTCATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAATGTCGAAGTGCGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCGCGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGCTCGTTCGGGCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCACGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAAGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGACGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCGCCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCGCCAGCCCTGATGATCGCCTGTGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193SY06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466

Multiple sequence alignment