Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OU5_RS15320 Genome accession   NZ_CP005960
Coordinates   3466635..3468716 (-) Length   693 a.a.
NCBI ID   WP_042932443.1    Uniprot ID   -
Organism   Pseudomonas mandelii JR-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3461635..3473716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU5_RS15305 (OU5_3050) - 3463251..3464849 (-) 1599 WP_010464417.1 SPOR domain-containing protein -
  OU5_RS15310 (OU5_3051) aroB 3464860..3465960 (-) 1101 WP_010464419.1 3-dehydroquinate synthase -
  OU5_RS15315 (OU5_3052) aroK 3466112..3466630 (-) 519 WP_010464421.1 shikimate kinase AroK -
  OU5_RS15320 (OU5_3053) pilQ 3466635..3468716 (-) 2082 WP_042932443.1 type IV pilus secretin PilQ Machinery gene
  OU5_RS15325 (OU5_3054) - 3468730..3469257 (-) 528 WP_010464425.1 pilus assembly protein PilP -
  OU5_RS15330 (OU5_3055) pilO 3469254..3469877 (-) 624 WP_010464427.1 type 4a pilus biogenesis protein PilO -
  OU5_RS15335 (OU5_3056) - 3469874..3470440 (-) 567 WP_010464430.1 PilN domain-containing protein -
  OU5_RS15340 (OU5_3057) comM 3470440..3471504 (-) 1065 WP_010464432.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75099.37 Da        Isoelectric Point: 5.7805

>NTDB_id=112634 OU5_RS15320 WP_042932443.1 3466635..3468716(-) (pilQ) [Pseudomonas mandelii JR-1]
MNRIFSTLGISLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTESPARIALDLPGVVNQLSSKSRE
LGGGNARSATVVEAKDRTRLIINLTQLTPYSARVDGNNLIVVVGQGANNKTPRPTTRAPRAATAMPAPTKAYAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFAKTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQISELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDLGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=112634 OU5_RS15320 WP_042932443.1 3466635..3468716(-) (pilQ) [Pseudomonas mandelii JR-1]
ATGAACAGGATTTTCTCAACCCTCGGTATCTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTTGCTGCGCTGCCGGGTGATCGTGTCGAGTTGAAGTTGTCTTTCGATGGCCCGCCCCCACAACCTCACG
GTTATACGACCGAGTCGCCGGCACGAATCGCGCTGGATCTGCCCGGGGTCGTCAATCAGCTTTCGAGCAAGAGTCGTGAG
CTGGGTGGTGGCAATGCCCGTAGCGCGACGGTGGTGGAAGCCAAGGACCGGACGCGGCTAATCATCAATCTGACCCAGTT
GACCCCGTACAGCGCCCGTGTCGACGGCAACAACCTGATTGTGGTGGTCGGGCAAGGTGCCAATAACAAAACGCCTCGGC
CGACTACCCGCGCGCCTCGCGCCGCGACTGCAATGCCCGCCCCGACGAAAGCCTATGCGCCCGTGGGCAAAGCCATTCGC
GGTGTGGATTTCCAGCGCGGTACGCAGGGCGAAGGCAATGTAGTGATTGATCTGTCGGATCCGTCCATCGCCCCGGACAT
CCAGGAGCGCGACGGAAAGATCATCCTGGGATTCGCCAAAACCCAATTGCCAGAACCGTTGCGCGTGCGCCTGGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGCCACGGGGGATCGGGCCACGATCAGTATCGAGCCCAGCGGA
GCCTTCGATTATTCGACCTACCAGACCGACAACAAGCTGACGGTCAGCATTCGGCCAATGACCGTCGACGACCTGCAAAA
GCGCAACGCCGAACGTTTTGCCTATACCGGTGAAAAACTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGTTTGCAGAAC
GTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGTAACGTCCTGCTGGT
GGCGCCGGCCGATGAAATCGCCGCGCGTGAACGTCAGGAACTGGAATCGCAGAAGCAGATTTCCGAGCTCGCGCCTTTGC
GTCGCGAATTGCTTCAGGTCAACTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCACGTCGGTGACCAGTGCCGAAGCG
AAAATCGACGAGCGGGGTTCGATCACGGTTGATGAACGGACCAACAACATCATTGCCTACCAGACCCAGGACCGACTCGA
CGAACTTCGTCGCATCGTCGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGAATCGTCGAAGCCAACG
TCGACTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACACATCCGGCGTCAGC
AATGGCTCGTCGACCACTATTGGTACTCCGGGCAGCACCAGCACCAACTCGCCGTTTGTCGACCTGGGCGCTGCGGGCAA
CACCTCGGGGATCGGCATCGCCTTCATCACCGATAACGTGTTGCTGGACCTGGAGCTGACCGCAATGGAGAAGACCGGTA
ACGGCGAAATCGTCTCACAACCCAAGGTGGTCACGTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACCGAGATTCCC
TATCAGGAAGCCAGTTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAAGCGTCGCTGTCCCTTGAGGTCACGCCGCAGAT
CACCCCGGACAACCGGATCATCATGGAGGTCAAGGTGACCAAGGATGAGCCGGACTACCTGAACAAGGTCCAGGATGTAC
CCCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATCGTGATTGGTGGGGTTTTCTCA
AATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.566

100

0.759
  comQ Acinetobacter baylyi ADP1

41.643

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.196

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.196

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.405

97.691

0.375
  pilQ Legionella pneumophila strain ERS1305867

37.982

97.258

0.369

Multiple sequence alignment