Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   OU5_RS15340 Genome accession   NZ_CP005960
Coordinates   3470440..3471504 (-) Length   354 a.a.
NCBI ID   WP_010464432.1    Uniprot ID   A0A024EBZ8
Organism   Pseudomonas mandelii JR-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3465440..3476504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU5_RS15315 (OU5_3052) aroK 3466112..3466630 (-) 519 WP_010464421.1 shikimate kinase AroK -
  OU5_RS15320 (OU5_3053) pilQ 3466635..3468716 (-) 2082 WP_042932443.1 type IV pilus secretin PilQ Machinery gene
  OU5_RS15325 (OU5_3054) - 3468730..3469257 (-) 528 WP_010464425.1 pilus assembly protein PilP -
  OU5_RS15330 (OU5_3055) pilO 3469254..3469877 (-) 624 WP_010464427.1 type 4a pilus biogenesis protein PilO -
  OU5_RS15335 (OU5_3056) - 3469874..3470440 (-) 567 WP_010464430.1 PilN domain-containing protein -
  OU5_RS15340 (OU5_3057) comM 3470440..3471504 (-) 1065 WP_010464432.1 pilus assembly protein PilM Machinery gene
  OU5_RS15345 (OU5_3058) - 3471721..3474165 (+) 2445 WP_370693249.1 penicillin-binding protein 1A -
  OU5_RS15350 (OU5_3059) - 3474421..3475689 (-) 1269 WP_010464435.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38173.71 Da        Isoelectric Point: 4.6091

>NTDB_id=112635 OU5_RS15340 WP_010464432.1 3470440..3471504(-) (comM) [Pseudomonas mandelii JR-1]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPTNAVVEKNIAELEGVGQALARVLLKAKTNLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGVSVRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLAGQLAVSQEGLTVAVVDIGATMTTLSVLYNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVFRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=112635 OU5_RS15340 WP_010464432.1 3470440..3471504(-) (comM) [Pseudomonas mandelii JR-1]
GTGCTAGGACTCTTCAATAAAAAAGCCAATACGCTTCTGGGCATCGACATCAGCTCCACGTCGGTGAAGTTGCTTGAGCT
AAGTCGCCAGGGTGAGCGTTATCGGGTCGAGGCCTATGCGGTCGAGCCCTTGCCTACCAACGCAGTGGTCGAAAAAAATA
TCGCCGAGCTCGAAGGCGTGGGCCAGGCACTCGCGCGCGTGCTGCTCAAGGCCAAAACCAACCTCAGGAATGTCGCCGTG
GCCGTCGCCGGTTCGGCAGTCATCACCAAAACCATCGAGATGGACGCAGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCCTATCCATTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTGTCGGTGC
GTAACCCCGAACGGGTCAATGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTGGAGGTGCGTGAAGCCGCCCTGGCGCTG
GCCGGCCTGACGGCGCGAGTGGTGGATGTCGAGGCCTATGCGCTTGAGCGGTCGTTCTCCTTGCTGGCGGGGCAACTGGC
GGTCTCTCAGGAGGGGCTGACCGTGGCGGTGGTCGACATCGGTGCGACCATGACCACCCTGAGCGTGCTGTACAACGGGC
GGATCATCTACACCCGGGAACAATTGTTCGGCGGTCGTCAGCTCACGGAGGAAATCCAGCGTCGTTATGGCCTGACCGTC
GAACAGGCGGGGCTGGCGAAGAAGCAGGGCGGGTTGCCGGATGACTACGTCAGCGAAGTCTTGCAACCGTTTCGCGATGC
GCTGGTGCAGCAGGTTTTCCGGTCGTTGCAGTTCTTCTTCGCATCCGGTCAGTACAACGCGGTCGATCACATCCTGCTGG
CTGGCGGTACCGCGTCGGTCCCAGGGCTTGATCGCCTGATCGAGCAGCGGTTGGGCACGCCAACCCAGGTGGCCAATCCG
TTCTCCGACATGGCATTGAGCAGCAAGGTTAACGCCGGGGCCCTGGCGAGCGATGCGCCAGCACTGATGATCGCTTGCGG
GCTCGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EBZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.627

100

0.576
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483

Multiple sequence alignment