Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   E0F31_RS06030 Genome accession   NZ_LR216064
Coordinates   1142720..1143568 (-) Length   282 a.a.
NCBI ID   WP_000705305.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 1137720..1148568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS06015 (SAMEA3714520_01128) - 1137958..1138317 (-) 360 WP_001237218.1 YbaN family protein -
  E0F31_RS06020 (SAMEA3714520_01129) topA 1138538..1140625 (-) 2088 WP_000179643.1 type I DNA topoisomerase -
  E0F31_RS06025 (SAMEA3714520_01130) - 1140793..1141836 (-) 1044 WP_001812883.1 TIGR00341 family protein -
  E0F31_RS12075 - 1142414..1142536 (+) 123 WP_001842489.1 hypothetical protein -
  E0F31_RS06030 (SAMEA3714520_01131) dprA/cilB/dalA 1142720..1143568 (-) 849 WP_000705305.1 DNA-processing protein DprA Machinery gene
  E0F31_RS06035 (SAMEA3714520_01132) - 1143666..1144355 (-) 690 WP_000643965.1 sugar phosphate nucleotidyltransferase -
  E0F31_RS06040 (SAMEA3714520_01133) - 1144367..1145245 (-) 879 WP_000837609.1 DMT family transporter -
  E0F31_RS06045 (SAMEA3714520_01134) licA 1145235..1146104 (-) 870 WP_000411207.1 choline kinase LicA -
  E0F31_RS06050 (SAMEA3714520_01135) - 1146121..1147143 (-) 1023 WP_000609888.1 ribitol-5-phosphate dehydrogenase -
  E0F31_RS06055 (SAMEA3714520_01136) - 1147148..1147855 (-) 708 WP_000638482.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31076.75 Da        Isoelectric Point: 5.4047

>NTDB_id=1126266 E0F31_RS06030 WP_000705305.1 1142720..1143568(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDILAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1126266 E0F31_RS06030 WP_000705305.1 1142720..1143568(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAATCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGTGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATATTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae D39

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae R6

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

99.645

100

0.996
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.227

100

0.982
  dprA/cilB/dalA Streptococcus mitis SK321

97.518

100

0.975
  dprA Streptococcus mutans UA159

63.571

99.291

0.631
  dprA Lactococcus lactis subsp. cremoris KW2

56.071

99.291

0.557
  dprA Staphylococcus aureus N315

38.163

100

0.383
  dprA Haemophilus influenzae Rd KW20

38.577

94.681

0.365

Multiple sequence alignment