Detailed information NTG1126108

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: E0F32_RS04500
• Genome accession: NZ_LR216050
• Coordinates: 826961..827611 (+)
• Length: 216 a.a.
• NCBI ID: WP_024477778.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 821961..832611

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F32_RS04455 (SAMEA3431333_00833)	-	822444..822659 (+)	216	WP_001232085.1	YozE family protein	-
E0F32_RS04460 (SAMEA3431333_00834)	-	822745..823713 (+)	969	WP_000658183.1	PhoH family protein	-
E0F32_RS04470	-	823906..824407 (+)	502	Protein_844	GNAT family N-acetyltransferase	-
E0F32_RS04475	-	824410..824735 (+)	326	Protein_845	TfoX/Sxy family protein	-
E0F32_RS11920	ald	825036..826147 (-)	1112	Protein_846	alanine dehydrogenase	-
E0F32_RS04495 (SAMEA3431333_00841)	-	826324..826893 (+)	570	WP_000443774.1	GNAT family N-acetyltransferase	-
E0F32_RS04500 (SAMEA3431333_00842)	comEA/celA/cilE	826961..827611 (+)	651	WP_024477778.1	ComEA family DNA-binding protein	Machinery gene
E0F32_RS04505 (SAMEA3431333_00843)	comEC/celB	827595..829835 (+)	2241	WP_130885934.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
E0F32_RS04510	-	830014..830202 (+)	189	WP_001808514.1	hypothetical protein	-
E0F32_RS04515 (SAMEA3431333_00844)	-	830235..830822 (+)	588	WP_000939895.1	ATP-binding cassette domain-containing protein	-
E0F32_RS04520 (SAMEA3431333_00845)	-	830826..832010 (+)	1185	WP_000655960.1	ABC transporter permease	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23243.72 Da
• Isoelectric Point: 6.0746

>NTDB_id=1126108 E0F32_RS04500 WP_024477778.1 826961..827611(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVKKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1126108 E0F32_RS04500 WP_024477778.1 826961..827611(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTCAAAGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAAGAAGGAAGAGAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAACTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae D39	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae R6	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.685	100	0.977
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis SK321	90.741	100	0.907
comEA	Lactococcus lactis subsp. cremoris KW2	41.333	100	0.431
comEA	Latilactobacillus sakei subsp. sakei 23K	33.476	100	0.361